FOXN1 antibody [C3], C-term

Host / ClonalityRabbit Polyclonal
ApplicationsWB
Species reactivityHuman

Cat No:

GTX104849

Package	Price	Qty
25 μl	USD 169.00
100 μl	USD 319.00

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Application Information: FOXN1 antibody [C3], C-term

Catalog Number

GTX104849

Product Name

FOXN1 antibody [C3], C-term

See all FOXN1 products

Applications

Application Note

	Suggested dilution	Reference
Western Blot (WB)	1:500-1:3000*

Not tested in other applications.
*Optimal dilutions/concentrations should be determined by the researcher.

Predicted Target Size

69 kDa (note)

Positive Controls

293T , A431 , HeLa , HepG2 , A549 , H1299 , HCT116

Form Supplied

Liquid

Concentration

0.8 mg/ml (Please refer to the vial label for the specific concentration)

Purification

Purified by antigen-affinity chromatography.

Specifications: FOXN1 antibody [C3], C-term

Full Name	forkhead box N1
Background	Mutations in the winged-helix transcription factor gene at the nude locus in mice and rats produce the pleiotropic phenotype of hairlessness and athymia, resulting in a severely compromised immune system. This gene is orthologous to the mouse and rat genes and encodes a similar DNA-binding transcription factor that is thought to regulate keratin gene expression. A mutation in this gene has been correlated with T-cell immunodeficiency, the skin disorder congenital alopecia, and nail dystrophy. Alternative splicing in the 5' UTR of this gene has been observed. [provided by RefSeq]
Synonyms	FKHL20 antibody, RONU antibody, WHN antibody, FOXN1 antibody, winged-helix nude antibody, winged-helix transcription factor nude antibody, Rowett nude antibody, winged helix nude antibody, forkhead box protein N1 antibody, forkhead box N1 antibody
Cellular Localization	Nucleus
Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Immunogen	Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human FOXN1. The exact sequence is proprietary.
Antigen Species	Human
Species Reactivity	Human
Predicted Cross Reactivity species Predicted cross-reactivity: Predicted cross-reactivity is based on sequence homology, with greater than 80% immunogen sequence identity considered positive. Please note that we are only able to guarantee products to work in applications and species in which they have been tested.	Bovine
Predict Reactivity Note	Bovine (93%)
Conjugation	Unconjugated

Storage Conditions: FOXN1 antibody [C3], C-term

Storage Buffer	1XPBS, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.
Storage Instruction	Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.
Notes	For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
ResearchArea
Stem Cell Development > Development > Morphogenesis More Hide

FOXN1 antibody [C3], C-term validated data

GTX104849 WB Image

Various tissue extracts (50 µg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with FOXN1 antibody [C3], C-term (GTX104849) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

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GTX104849 WB Image

FOXN1 antibody [C3], C-term detects FOXN1 protein by western blot analysis. Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with FOXN1 antibody [C3], C-term (GTX104849) diluted at 1:500.

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GTX104849 WB Image

FOXN1 antibody [C3], C-term detects FOXN1 protein by western blot analysis. Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with FOXN1 antibody [C3], C-term (GTX104849) diluted at 1:500.

Top

GTX104849 WB Image

Various tissue extracts (50 µg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with FOXN1 antibody [C3], C-term (GTX104849) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Top

GTX104849 WB Image

FOXN1 antibody [C3], C-term detects FOXN1 protein by western blot analysis. Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with FOXN1 antibody [C3], C-term (GTX104849) diluted at 1:500.

Top

GTX104849 WB Image

FOXN1 antibody [C3], C-term detects FOXN1 protein by western blot analysis. Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with FOXN1 antibody [C3], C-term (GTX104849) diluted at 1:500.

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Target Bioinfo/ Predicted Isoform Cross Reactivity
Species	Gene name	Gene ID	SwissProt	OMIM	Isoform (mRNA)	Protein Accession#	MW
Homo sapiens	FOXN1	8456	O15353, B2R9Q7	600838, 601705	NM_003593	NP_003584	68.94

*MSDS

MSDS-Glycerol.pdf

MSDS-PBS.pdf

MSDS-Thimerosal.pdf

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FOXN1 antibody [C3], C-term

FOXN1 antibody [C3], C-term

Application Information: FOXN1 antibody [C3], C-term

Specifications: FOXN1 antibody [C3], C-term

Storage Conditions: FOXN1 antibody [C3], C-term