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FOXP3 antibody [C3], C-term

Anti-FOXP3 antibody [C3], C-term used in Western Blot (WB). GTX107737
Anti-FOXP3 antibody [C3], C-term used in IHC (Paraffin sections) (IHC-P). GTX107737
Anti-FOXP3 antibody [C3], C-term used in Immunoprecipitation (IP). GTX107737
Anti-FOXP3 antibody [C3], C-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX107737

Cat. No. GTX107737

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, ICC/IF, IHC-P, IP

Reactivity

Human, Mouse
Package
100 μl ($399),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
IP 1:100-1:500
Not tested in other applications.

Calculated MW

47 kDa. ( Note )

Positive Control

Mouse brain

Predict Reactivity

Rat, Sheep, Bovine, Cat, Pig, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Mouse

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of mouse FOXP3. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1240836

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

forkhead box P3 , JM2 , scurfin , sf

Cellular Localization

Nucleus

Background

Probable transcription factor. Plays a critical role in the control of immune response.

Database

Research Area

DATA IMAGES

Anti-FOXP3 antibody [C3], C-term used in Western Blot (WB). GTX107737

GTX107737 WB Image

Sample (50 ug of whole cell lysate)
A: mouse brain
10% SDS PAGE
GTX107737 diluted at 1:1000

Anti-FOXP3 antibody [C3], C-term used in IHC (Paraffin sections) (IHC-P). GTX107737

GTX107737 IHC-P Image

FOXP3 antibody [C3], C-term detects FOXP3 protein at cytoplasm and nucleus in mouse spleen by immunohistochemical analysis.
Sample: Paraffin-embedded mouse spleen.
FOXP3 antibody [C3], C-term (GTX107737) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-FOXP3 antibody [C3], C-term used in Immunoprecipitation (IP). GTX107737

GTX107737 IP Image

FOXP3 antibody [C3], C-term immunoprecipitates FOXP3 protein in IP experiments.
IP samples: Jurkat whole cell extract
A. Control with 4 μg of preimmune Rabbit IgG
B. Immunoprecipitation of FOXP3 protein by 4 μg FOXP3 antibody [C3], C-term (GTX107737)
10 % SDS-PAGE
The immunoprecipitated FOXP3 protein was detected by FOXP3 antibody [C3], C-term (GTX107737) diluted at 1:500.
[EasyBlot anti-rabbit IgG (GTX221666-01) was used as a secondary reagent]

Anti-FOXP3 antibody [C3], C-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX107737

GTX107737 ICC/IF Image

FOXP3 antibody [C3], C-term detects FOXP3 protein at nucleus by immunofluorescent analysis.
Sample: SK-N-SH cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: FOXP3 protein stained by FOXP3 antibody [C3], C-term (GTX107737) diluted at 1:500.
Red: phalloidin, a cytoskeleton marker, diluted at 1:200.
Scale bar = 10 μm.

REFERENCE

REVIEW

FOXP3 antibody [C3], C-term Cat. No. GTX107737

Rating ( Average 5 based on 3 users reviews)
Application
IHC (Paraffin sections)(IHC-P) ( Average 5 based on 3 users reviews)
Date : Anonymous submitted on 13-May-2021
Score :
Application Tested : IHC-P
Sample Species : Ms
Sample : Intestine tumor
Fixation : 4% PFA in PBS
Permeabilization /Antigen retrieval : Tris-EDTA pH9, boil°C, 0.5Hr
Primary Antibody : 1:100, 4°C, 16Hr
Date : Anonymous submitted on 04-May-2021
Score :
Application Tested : IHC-P
Sample Species : Ms
Sample : Lung tumor tissue
Fixation : 4% PFA in PBS, 25°C, 0.5Hr
Permeabilization /Antigen retrieval : Citrate buffer, pH 6.0, 0.25Hr
Primary Antibody : 1:300, 4°C, 12Hr
Figure Note : FOXP3 antibody detects FOXP3 protein at cytoplasm and nucleus in the mouse lung tumor by immunohistochemical analysis. Sample: Paraffin-embedded mouse lung tumor. FOXP3 antibody (GTX107737) diluted at 1:300. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
Date : Anonymous submitted on 15-Dec-2020
Score :
Application Tested : IHC-P
Sample Species : Ms
Sample : mammary carcinoma
Blocking : Dako REAL Antibody Diluent, 25°C, 0.5Hr
Fixation : 10% phosphate buffered formalin, 25°C, 16Hr
Permeabilization /Antigen retrieval : 0.05% tween 20 in PBST/Citrate buffer (pH 6.0)
Primary Antibody : 1:100, 25°C, 1Hr
Figure Note : Red: FOXP3
Blue: Nuclear
Application Reference
Nguyen DJM et al. Mol Cancer Res 2019; (Epub)
Application : WB
Reactivity : Human
SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169