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FRMPD1 antibody

Anti-FRMPD1 antibody used in Western Blot (WB). GTX85011
Anti-FRMPD1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX85011

Cat. No. GTX85011

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, ELISA

Reactivity

 Human
Package
100 μg ($399)
See all FRMPD1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1 - 2 μg/mL
ICC/IF 20 μg/mL
ELISA Assay dependent
Not tested in other applications.

Calculated MW

173 kDa. ( Note )

Positive Control

K-562 Cell Slide(GTX25535) , K562 Cell Lysate(GTX27911)

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

FRMPD1 antibody was raised against a 15 amino acid synthetic peptide from near the carboxy terminus of human FRMPD1.The immunogen is located within amino acids 1330 - 1380 of FRMPD1.

Purification

Purified by antigen-affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

FERM and PDZ domain containing 1 , FRMD2

Cellular Localization

Cytoplasm, cytosol,Cell membrane

Background

The FERM and PDZ domain containing (FRMPD) protein family consists of four proteins that contain a FERM (Four-point-one, erzin, radixin, moesin) domain and at least one PDZ (PSD-95/Discs large/Zonula-occuldens-1) domain. FRMPD1 was initially identified through a yeast two-hybrid screening with Activator of G-protein signaling 3 (AGS3), a protein that acts to stabilize the GDP-bound conformation of Gai, as the bait. FRMPD1 binds to the tetratricopeptide repeat of AGS3 and stabilizes AGS3 in a membrane environment. Suppression of FRMPD1 in Cath.a-differentiated neuronal cells decreased the level of endogenous AGS3 in membrane fractions by ~50% and enhanced the inhibition of forskolin-induced increases in cAMP. Co-immunoprecipitation studies indicate that the interaction of AGS3 with FRMPD1 is mutually exclusive with the interaction of AGS3 with Gai3, suggesting that FRMPD1 may position AGS3 in the membrane where it can then interact with Galphai.

Database

Research Area

DATA IMAGES

Anti-FRMPD1 antibody used in Western Blot (WB). GTX85011

GTX85011 WB Image

WB analysis of K562 cell lysate using GTX85011 FRMPD1 antibody.
Working concentration : (A) 1 and (B) 2 μg/ml

Anti-FRMPD1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX85011

GTX85011 ICC/IF Image

ICC/IF analysis of K562 cells using GTX85011 FRMPD1 antibody.
Working concentration : 20 μg/ml

REFERENCE

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REVIEW

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SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399