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SFRP2 antibody [C2C3], C-term

Anti-SFRP2 antibody [C2C3], C-term used in Western Blot (WB). GTX111892
Anti-SFRP2 antibody [C2C3], C-term used in IHC (Paraffin sections) (IHC-P). GTX111892
Anti-SFRP2 antibody [C2C3], C-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX111892

Cat. No. GTX111892

Reference 2

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Pig
Package
100 μl ($399),
25 μl ($169)
See all SFRP2 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

33 kDa. ( Note )

Predict Reactivity

Mouse, Rat, Zebrafish, Sheep, Bovine, Dog, Chicken, Xenopus tropicalis, Chimpanzee(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.12 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human SFRP2. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2037978

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

secreted frizzled related protein 2 , FRP-2 , SARP1 , SDF-5

Background

This gene encodes a member of the SFRP family that contains a cysteine-rich domain homologous to the putative Wnt-binding site of Frizzled proteins. SFRPs act as soluble modulators of Wnt signaling. Methylation of this gene is a potential marker for the presence of colorectal cancer. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-SFRP2 antibody [C2C3], C-term used in Western Blot (WB). GTX111892

GTX111892 WB Image

Various whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with SFRP2 antibody [C2C3], C-term (GTX111892) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-SFRP2 antibody [C2C3], C-term used in IHC (Paraffin sections) (IHC-P). GTX111892

GTX111892 IHC-P Image

Immunohistochemical analysis of paraffin-embedded MCF7 xenograft, using FRP 2(GTX111892) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-SFRP2 antibody [C2C3], C-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX111892

GTX111892 ICC/IF Image

SFRP2 antibody [C2C3], C-term detects SFRP2 protein by immunofluorescent analysis.
Sample: HCT 116 cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: SFRP2 protein stained by SFRP2 antibody [C2C3], C-term (GTX111892) diluted at 1:500.
Blue: Hoechst 33342 staining.

REFERENCE

Application Reference

Ledwon JK et al. J Cell Mol Med 2022; (Epub) Langerhans cells and SFRP2/Wnt/beta-catenin signalling control adaptation of skin epidermis to mechanical stretching.
Application : IHC-P
Reactivity : Pig
Khan K et al. Front Cell Dev Biol 2020; 8 : 862 The Role of Wnt/β-Catenin Pathway Mediators in Aortic Valve Stenosis.
Application : WB, IHC-P
Reactivity : Human
Submit a Reference

REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 399
$ 169