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  • 58K Golgi protein antibody [N1N3]

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1 .GTX109972 58K Golgi protein antibody [N1N3]

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58K Golgi protein antibody [N1N3]


  • Host / ClonalityRabbit Polyclonal
  • ApplicationsIHC-P, WB
  • Species reactivityHuman, Mouse
  • ★★★★★   (  1  ) Publication  ( 1 )
Cat No: GTX109972
Package Price Qty
25 μlUSD 119.00
100 μlUSD 319.00
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  • Anti-58K Golgi protein antibody [N1N3] used in IHC (Formalin-fixed paraffin-embedded sections). GTX109972
  • Anti-58K Golgi protein antibody [N1N3] used in Western Blot (WB). GTX109972
  • Anti-58K Golgi protein antibody [N1N3] used in Western Blot (WB). GTX109972
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  • Datasheet
  • Star Review (1)
    Reference (1)
  • Bio-info
  • Protocol & MSDS
  • Application Information: 58K Golgi protein antibody [N1N3]

Catalog Number GTX109972
★★★★★
( 1 )
Publication ( 1 )
Product Name 58K Golgi protein antibody [N1N3]
See all 58K Golgi protein products
ApplicationsIHC-P, WB
Application Note
Suggested dilutionReference
IHC (Formalin-fixed paraffin-embedded sections)1:100-1:1000*  
Western Blot (WB)1:500-1:3000* Y
Not tested in other applications.
*Optimal dilutions/concentrations should be determined by the researcher.
Predicted Target Size59 kDa (note) IsoForm (3)
Positive ControlsHepG2 , mouse liver , *MDA-MB-231
Form SuppliedLiquid
Concentration0.98 mg/ml (Please refer to the vial label for the specific concentration)
PurificationPurified by antigen-affinity chromatography.

  • Specifications: 58K Golgi protein antibody [N1N3]

Full Nameformiminotransferase cyclodeaminase
Background58K Golgi protein is a bifunctional enzyme that channels 1-carbon units from formiminoglutamate, a metabolite of the histidine degradation pathway, to the folate pool.[supplied by OMIM]
SynonymsLCHC1 antibody, FTCD antibody, formiminotransferase-cyclodeaminase antibody, formimidoyltransferase cyclodeaminase antibody, formimidoyltransferase-cyclodeaminase antibody, formiminotransferase cyclodeaminase antibody
Cellular LocalizationCytoplasm (By similarity) , Golgi apparatus
HostRabbit
ClonalityPolyclonal
IsotypeIgG
ImmunogenRecombinant protein encompassing a sequence within the center region of human 58K Golgi protein. The exact sequence is proprietary.
Antigen SpeciesHuman
Species ReactivityHuman, Mouse
Predicted Cross Reactivity species Predicted cross-reactivity:
    Predicted cross-reactivity is based on sequence homology, with greater than 80% immunogen sequence identity considered positive.
    Please note that we are only able to guarantee products to work in applications and species in which they have been tested.
Rat, Pig, Chicken, Bovine
Predict Reactivity NoteRat (89%), Pig (84%), Chicken (80%), Bovine (88%)
ConjugationUnconjugated

  • Storage Conditions: 58K Golgi protein antibody [N1N3]

Storage Buffer0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.
Storage InstructionKeep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
NotesFor In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
ResearchArea
  • Cell Biology > Cytoskeleton
  • Signal Transduction > ECM/Cytoskeleton > Cytoskeleton
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58K Golgi protein antibody [N1N3] validated data
  • Anti-58K Golgi protein antibody [N1N3] used in IHC (Formalin-fixed paraffin-embedded sections). GTX109972
    • GTX109972 IHC-P Image
      FTCD antibody [N1N3] detects FTCD protein at cytoplasm in human hepatocellular carcinoma by immunohistochemical analysis.
      Sample: Paraffin-embedded human hepatocellular carcinoma.
      FTCD antibody [N1N3] (GTX109972) diluted at 1:500.
      Top
  • Anti-58K Golgi protein antibody [N1N3] used in Western Blot (WB). GTX109972
    • GTX109972 WB Image
      Sample (50 ug of whole cell lysate)
      A: mouse liver
      7.5% SDS PAGE
      GTX109972 diluted at 1:1000
      Top
  • Anti-58K Golgi protein antibody [N1N3] used in Western Blot (WB). GTX109972
    • GTX109972 WB Image
      Sample (30 ug of whole cell lysate)
      A: Hep G2 (GTX27900)
      7.5% SDS PAGE
      GTX109972 diluted at 1:1000
      Top
  • Anti-58K Golgi protein antibody [N1N3] used in IHC (Formalin-fixed paraffin-embedded sections). GTX109972
    • GTX109972 IHC-P Image
      FTCD antibody [N1N3] detects FTCD protein at cytoplasm in human hepatocellular carcinoma by immunohistochemical analysis.
      Sample: Paraffin-embedded human hepatocellular carcinoma.
      FTCD antibody [N1N3] (GTX109972) diluted at 1:500.
      Top
  • Anti-58K Golgi protein antibody [N1N3] used in Western Blot (WB). GTX109972
    • GTX109972 WB Image
      Sample (50 ug of whole cell lysate)
      A: mouse liver
      7.5% SDS PAGE
      GTX109972 diluted at 1:1000
      Top
  • Anti-58K Golgi protein antibody [N1N3] used in Western Blot (WB). GTX109972
    • GTX109972 WB Image
      Sample (30 ug of whole cell lysate)
      A: Hep G2 (GTX27900)
      7.5% SDS PAGE
      GTX109972 diluted at 1:1000
      Top
  • StarReview
  • 58K Golgi protein antibody [N1N3]

  • Rating : ★★★★★ ( Average 5.0 based on 1 users reviews)
  • Western Blot (WB)(1) :★★★★★
  • Anonymous StarReview submitted on 25 Dec 2014
  • Score : ★★★★★
  • Application Tested : WB
  • Sample Species : Human
  • Sample : MDA-MB-231 cells
  • Amount used(ug/cells) : 20ug
  • Blocking conditions
  • Blocking Buffer : 5% non-fat milk
  • Incubation Time & Temperature : RT (℃)/1hr
  • Primary antibody
  • Dilution : 1:3000( conc. of dilution )
  • Incubation Time & Temperature : 4 (℃)/overnight
Back to Top
  • Reference for 58K Golgi protein antibody [N1N3]
Application References Application Reactivity
Yu Z et al. Using a yeast two-hybrid system to identify FTCD as a new regulator for HIF-1α in HepG2 cells. Cell Signal 26 (7):1560-1566 (2014). WB Human
Target Bioinfo/ Predicted Isoform Cross Reactivity
SpeciesGene nameGene IDSwissProtOMIMIsoform (mRNA)Protein Accession#MW
Homo sapiensFTCD10841O95954, B9EGD0229100, 606806NP_00130734161.29
Homo sapiensFTCD10841O95954, B9EGD0229100, 606806NM_006657NP_00664858.94
Homo sapiensFTCD10841O95954, B9EGD0229100, 606806NM_206965NP_99684858.94

Cross-react Bioinfo
SpeciesGene nameGene IDSwissProtIsoform (mRNA)Protein Accession#MW
Mus musculusFtcd14317Q91XD4NM_080845NP_54312158.95
Rattus norvegicusFtcd89833O88618NM_053567NP_44601958.92
    *MSDS
    MSDS-Glycerol.pdf
    MSDS-Thimerosal.pdf
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