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FYN antibody [N1N3]

Anti-FYN antibody [N1N3] used in Western Blot (WB). GTX101189
Anti-FYN antibody [N1N3] used in IHC (Whole mount) (IHC-Wm). GTX101189
Anti-FYN antibody [N1N3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX101189
Anti-FYN antibody [N1N3] used in Western Blot (WB). GTX101189

Cat. No. GTX101189

Reference 4

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-Wm

Reactivity

 Human, Mouse, Zebrafish
Package
100 μl ($399),
25 μl ($169)
See all FYN products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-Wm Assay dependent
Not tested in other applications.

Calculated MW

61 kDa. ( Note )

Positive Control

A431 , H1299

Predict Reactivity

Rat, Bovine, Chicken, Pig(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 40% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human FYN. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1240845

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

FYN proto-oncogene, Src family tyrosine kinase , SLK , SYN , p59-FYN

Cellular Localization

Cell membrane

Background

This gene is a member of the protein-tyrosine kinase oncogene family. It encodes a membrane-associated tyrosine kinase that has been implicated in the control of cell growth. The protein associates with the p85 subunit of phosphatidylinositol 3-kinase and interacts with the fyn-binding protein. Alternatively spliced transcript variants encoding distinct isoforms exist. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-FYN antibody [N1N3] used in Western Blot (WB). GTX101189

GTX101189 WB Image

Whole zebrafish extract (30 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with FYN antibody [N1N3] (GTX101189) diluted at 1:1000.

Anti-FYN antibody [N1N3] used in IHC (Whole mount) (IHC-Wm). GTX101189

GTX101189 IHC-Wm Image

FYN antibody [N1N3] detects FYN protein on whole-mount zebrafish embryos by immunohistochemical analysis.
Sample: Paraformaldehyde-fixed zebrafish embryos.
FYN antibody [N1N3] (GTX101189) dilution: 1:200.
Anti-FYN antibody [N1N3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX101189

GTX101189 ICC/IF Image

Immunofluorescence analysis of paraformaldehyde-fixed A431, using FYN(GTX101189) antibody at 1:200 dilution.

Anti-FYN antibody [N1N3] used in Western Blot (WB). GTX101189

GTX101189 WB Image

Sample (30 ug of whole cell lysate)
A: A431 (GTX27909)
B: H1299
7.5% SDS PAGE
GTX101189 diluted at 1:1000

REFERENCE

Application Reference

Terasaki M et al. Nutr Cancer 2021; : 1-16 A Marine Carotenoid of Fucoxanthinol Accelerates the Growth of Human Pancreatic Cancer PANC-1 Cells.
Huang C et al. J Agric Food Chem 2019; 67 (10) : 2818-2830 Ampelopsins A and C Induce Apoptosis and Metastasis through Downregulating AxL, TYRO3, and FYN Expressions in MDA-MB-231 Breast Cancer Cells.
Application : WB
Reactivity : Human
Gingras J et al. J Cell Sci 2016; 129 (5) : 898-911 α-Dystrobrevin-1 recruits Grb2 and α-catulin to organize neurotransmitter receptors at the neuromuscular junction.
Application : WB
Reactivity : Mouse
Chang SF et al. Proc Natl Acad Sci U S A 2014; 111 (5) : 1855-60 Different modes of endothelial-smooth muscle cell interaction elicit differential β-catenin phosphorylations and endothelial functions.
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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169