FZD8 / frizzled 8 antibody, C-term
|Applications||IHC, IHC-P, WB|
|Application Note||Approx 75kDa band observed in human heart and human pancreas lysates (calculated MW of 73.3kDa according to NP_114072.1). Recommended concentration: 1-3µg/ml. In paraffin embedded Human Pancreas, shows granular cytoplasmic staining of acinar cells. Cells of the Islets of Langerhans are weakly stained, if at all. Recommended concentration, 3µg/ml. |
|Positive Controls||The peptide used to product this antibody is available for purchase (Catalog Number:GTX89473-PEP).|
(Please refer to the vial label for the specific concentration)|
|Purification||Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.|
|Full Name||frizzled homolog 8 (Drosophila)|
|Product Description||Goat polyclonal antibody to FZD8 / frizzled 8|
|Background||Receptor for Wnt proteins. Component of the Wnt-Fzd-LRP5-LRP6 complex that triggers beta-catenin signaling through inducing aggregation of receptor-ligand complexes into ribosome-sized signalsomes. The beta-catenin canonical signaling pathway leads to the activation of disheveled proteins, inhibition of GSK-3 kinase, nuclear accumulation of beta-catenin and activation of Wnt target genes. A second signaling pathway involving PKC and calcium fluxes has been seen for some family members, but it is not yet clear if it represents a distinct pathway or if it can be integrated in the canonical pathway, as PKC seems to be required for Wnt-mediated inactivation of GSK-3 kinase. Both pathways seem to involve interactions with G-proteins. May be involved in transduction and intercellular transmission of polarity information during tissue morphogenesis and/or in differentiated tissues. |
|Synonyms||frizzled (Drosophila) homolog8, frizzled8, frizzled homolog 8 (Drosophila), FZD8 / frizzled8, frizzled homolog8 (Drosophila), frizzled 8, frizzled (Drosophila) homolog 8, HGNC:4046, hFZ8, FZD8 / frizzled 8, FZD8, FZ-8, FZ8, FZ 8|
|Cellular Localization||Colocalizes with GOPC at the Golgi apparatus. |
|Specificity||This antibody is specific for FZD8 / frizzled 8.|
|Predicted Cross Reactivity species Predicted cross-reactivity:|
Predicted cross-reactivity is based on sequence homology, with greater than 80% immunogen sequence identity considered positive.
Please note that we are only able to guarantee products to work in applications and species in which they have been tested.
|Mouse, Rat, Dog, Bovine|