Feline Immunodeficiency Virus p24 gag antibody [PAK3-2C1]
|Applications||ELISA, FACS, ICC/IF, IHC-Fr, WB|
| ELISA||Assay-dependent dilution
| Flow cytometry/FACS||Assay-dependent dilution
| Immunocytochemistry/ Immunofluorescence||Assay-dependent dilution
| IHC (Frozen sections)||Assay-dependent dilution
| Western blot||Assay-dependent dilution
Flow cytometry/FACS : Membrane permeabilisation is required for this application. Use 10μl of the suggested working dilution to label 106 cells in 100μl.Not tested in other applications.
IHC (Frozen sections) : The epitope recognised by this antibody is reported to be sensitive to formaldehyde fixation and tissue processing. Recommend the use of acetone fixation for frozen sections.
Western blot : Thus antibody detects a dominant band of approximately 24kDa, and also detects p24 gag precursor bands at 36, 39, 49and 52kDa under reducing conditions.
*Optimal dilutions/concentrations should be determined by the researcher.
(Please refer to the vial label for the specific concentration)|
|Purification||Protein A purified|
|Purification Note||From tissue culture supernatant|