Flotillin 2 antibody [N1C1]

Name: Flotillin 2 antibody [N1C1]
Brand: GeneTex
SKU: GTX114411
Price: 399.00 USD
Rating: 3 (1 reviews)

Anti-Flotillin 2 antibody [N1C1] used in Western Blot (WB). GTX114411

Anti-Flotillin 2 antibody [N1C1] used in Immunoprecipitation (IP). GTX114411

Anti-Flotillin 2 antibody [N1C1] used in IHC (Paraffin sections) (IHC-P). GTX114411

Cat. No. GTX114411

Reference 2

Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Application	WB, IHC-P, IP
Reactivity	Human, Mouse
Package	100 μl ($399), 25 μl ($169)

See all Flotillin 2 products

View product citations for antibody GTX114411 on CiteAb

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Recommended Dilution
WB	1:500-1:3000
IHC-P	1:100-1:1000
IP	1:100-1:500

Not tested in other applications.

Calculated MW

47 kDa. ( Note )

Positive Control

Mouse brain , HCT116

Predict Reactivity

Rat, Bovine, Xenopus tropicalis(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.49 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human Flotillin 2. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_10619212

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

flotillin 2 , ECS-1 , ECS1 , ESA , ESA1 , M17S1

Cellular Localization

Cell membrane; Peripheral membrane protein , Membrane , caveola; Peripheral membrane protein

Background

Caveolae are small domains on the inner cell membrane involved in vesicular trafficking and signal transduction. This gene encodes a caveolae-associated, integral membrane protein, which is thought to function in neuronal signaling. [provided by RefSeq]

Database

Gene ID: 2319 FLOT2
UniProt: Q14254 FLOT2

Research Area

DATA IMAGES

GTX114411 WB Image

Sample (50 ug of whole cell lysate)
A: mouse brain
10% SDS PAGE
GTX114411 diluted at 1:1000

GTX114411 WB Image

Sample (30 ug of whole cell lysate)
A: HCT116
10% SDS PAGE
Flotillin-2 antibody
GTX114411 diluted at 1:1000

GTX114411 IP Image

Flotillin 2 antibody [N1C1] immunoprecipitates Flotillin 2 protein in IP experiments.
IP samples: HCT-116 whole cell extract
A. Control with 4 μg of preimmune Rabbit IgG
B. Immunoprecipitation of Flotillin 2 protein by 4 μg Flotillin 2 antibody [N1C1] (GTX114411)
10 % SDS-PAGE
The immunoprecipitated Flotillin 2 protein was detected by Flotillin 2 antibody [N1C1] (GTX114411) diluted at 1:1000.
[EasyBlot anti-rabbit IgG (GTX221666-01) was used as a secondary reagent]

GTX114411 IHC-P Image

Immunohistochemical analysis of paraffin-embedded BT474 xenograft, using Flotillin 2(GTX114411) antibody at 1:100 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

REFERENCE

Application Reference

Zhong Z et al. JCI Insight 2023; 8 (19) RhoA vesicle trafficking-mediated transglutaminase 2 membrane translocation promotes IgA1 mesangial deposition in IgA nephropathy.

Butko MT et al. Proc Natl Acad Sci U S A 2013; 110 (8) : E726-35 In vivo quantitative proteomics of somatosensory cortical synapses shows which protein levels are modulated by sensory deprivation.

Application : WB

Reactivity : Mouse

Submit a Reference

REVIEW