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G3BP1 (phospho Ser232) antibody

Anti-G3BP1 (phospho Ser232) antibody used in Western Blot (WB). GTX79070
Anti-G3BP1 (phospho Ser232) antibody used in IHC (Paraffin sections) (IHC-P). GTX79070

Cat. No. GTX79070

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, IHC-P

Reactivity

Human
Package
100 μg ($399)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500 - 1:1000
IHC-P 1:50 - 1:100
Not tested in other applications.

Calculated MW

52 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS (without Mg²⁺ and Ca²⁺) pH7.4, 150mM NaCl, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

The antiserum was produced against synthesized phosphopeptide derived from human G3BP-1 around the phosphorylation site of serine 232 (S-S-Sp-P-A).

Purification

Purified by sequential chromatography on phospho- and non-phospho-peptide affinity columns.
From serum

Conjugation

Unconjugated

RRID

AB_11168970

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

G3BP,G3BP stress granule assembly factor 1,G3BP1,HDHVIII,G3BP-1

Cellular Localization

Cytoplasm,Cytosol,Cytoplasmic granule,Cell membrane,Nucleus

Background

This gene encodes one of the DNA-unwinding enzymes which prefers partially unwound 3'-tailed substrates and can also unwind partial RNA/DNA and RNA/RNA duplexes in an ATP-dependent fashion. This enzyme is a member of the heterogeneous nuclear RNA-binding proteins and is also an element of the Ras signal transduction pathway. It binds specifically to the Ras-GTPase-activating protein by associating with its SH3 domain. Several alternatively spliced transcript variants of this gene have been described, but the full-length nature of some of these variants has not been determined. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-G3BP1 (phospho Ser232) antibody used in Western Blot (WB). GTX79070

GTX79070 WB Image

WB analysis of 293 cell lysate using GTX79070 G3BP1 (phospho Ser232) antibody (Lane 3 and 4) and G3BP1 antibody (Lane 1 and 2).

Anti-G3BP1 (phospho Ser232) antibody used in IHC (Paraffin sections) (IHC-P). GTX79070

GTX79070 IHC-P Image

IHC-P analysis of human breast carcinoma tissue using GTX79070 G3BP1 (phospho Ser232) antibody.

REFERENCE

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REVIEW

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Package List Price ($)
$ 399