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G3BP1 antibody, N-term

Anti-G3BP1 antibody, N-term used in IHC (Paraffin sections) (IHC-P). GTX47440
Anti-G3BP1 antibody, N-term used in Immunoprecipitation (IP). GTX47440
Anti-G3BP1 antibody, N-term used in Western Blot (WB). GTX47440

Cat. No. GTX47440

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P, IP

Reactivity

 Human, Mouse, Rat
Package
50 μg ($399)
See all G3BP1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 0.2-2.5 ug/ml
IHC-P 2-10 ug/ml
IP Assay dependent
Not tested in other applications.

Calculated MW

52 kDa. ( Note )

Positive Control

mouse brain , NT-2

PROPERTIES

Form

Liquid

Buffer

PBS, 2% Sucrose

Preservative

0.09% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.5-1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

A synthetic peptide corresponding to a N-terminal region of Human G3BP1

Purification

Affinity Purified

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

G3BP stress granule assembly factor 1 , G3BP , HDH-VIII

Cellular Localization

Cytoplasm, cytosol,Nucleus

Background

This gene encodes one of the DNA-unwinding enzymes which prefers partially unwound 3'-tailed substrates and can also unwind partial RNA/DNA and RNA/RNA duplexes in an ATP-dependent fashion. This enzyme is a member of the heterogeneous nuclear RNA-binding proteins and is also an element of the Ras signal transduction pathway. It binds specifically to the Ras-GTPase-activating protein by associating with its SH3 domain. Several alternatively spliced transcript variants of this gene have been described, but the full-length nature of some of these variants has not been determined. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-G3BP1 antibody, N-term used in IHC (Paraffin sections) (IHC-P). GTX47440

GTX47440 IHC-P Image

IHC-P analysis of human heart tissue using GTX47440 G3BP1 antibody at 1:100. Left to right : DAPI, G3BP1, Merge. Heat-induced antigen retrieval method utilizing Sodium Citrate buffer.

Anti-G3BP1 antibody, N-term used in Immunoprecipitation (IP). GTX47440

GTX47440 IP Image

IP analysis of rat brain tissue using GTX47440 G3BP1 antibody at 1:500. IP antibody: 6μg. IP buffer: 125 mM NaCl, 20mM Tris, pH7.4,1mM MagCl2, 2% CHAP, 10% Glycerol, and protease inhibitor mixture.

Anti-G3BP1 antibody, N-term used in Western Blot (WB). GTX47440

GTX47440 WB Image

WB analysis of NT-2 cells and mouse brain using GTX47440 G3BP1 antibody at 2μg/ml.
Lane 1: Human NT-2 cells lysate (60μg),
Lane 2: Mouse brain extract (80μg)

REFERENCE

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REVIEW

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SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399