APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 1:500-1:3000 |
| 1:100-1:1000 |
Not tested in other applications.
Calculated MW
Positive Control
human GABARAPL1-transfected 293T cells
Predict Reactivity
Mouse, Rat, Rabbit, Bovine, Guinea pig, Pig, Xenopus tropicalis, Rhesus Monkey(>80% identity)
PROPERTIES
Form
Liquid
Buffer
PBS, 20% Glycerol
Preservative
0.025% ProClin 300
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
1 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Full length human GABARAPL1 Recombinant protein.
Purification
Purified by antigen-affinity chromatography.
Conjugation
Unconjugated
RRID
AB_2885951
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
GABA type A receptor associated protein like 1 , APG8-LIKE , APG8L , ATG8 , ATG8B , ATG8L , GEC1
Cellular Localization
Cytoplasm , Membrane , Endoplasmic reticulum , Golgi apparatus
Background
Increases cell-surface expression of kappa-type opioid receptor through facilitating anterograde intracellular trafficking of the receptor.
Database
Research Area
DATA IMAGES
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GTX129277 ICC/IF Image
GABARAPL1 antibody detects GABARAPL1 protein at autophagosome by immunofluorescent analysis.
Samples: HeLa cells mock (left) and treated with 50μM Chloroquine for 24 hr (right) were fixed in 4% paraformaldehyde at RT for 15 min.
Green: GABARAPL1 protein stained by GABARAPL1 antibody (GTX129277) diluted at 1:1000.
Red: Phalloidin, a F-actin marker.
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GTX129277 WB Image
Untreated (–) and treated (+) HeLa whole cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membranes were blotted with GABARAPL1 antibody (GTX129277) diluted at 1:500 and competitor's antibody diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
*The competitor is not affiliated with GeneTex and does not endorse this product.
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GTX129277 WB Image
GABARAPL1 antibody detects GABARAPL1 protein by western blot analysis.
A. 30 μg 293T whole cell lysate/extract
B. 30 μg whole cell lysate/extract of human GABARAPL1-transfected 293T cells
15 % SDS-PAGE
GABARAPL1 antibody (GTX129277) dilution: 1:1000
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REFERENCE
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REVIEW
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