|Applications||ICC/IF, IHC-Fr, IHC-P, WB|
Not tested in other applications.
|IHC (Frozen sections)||1:100-1:1000*
|IHC (Formalin-fixed paraffin-embedded sections)||1:100-1:1000*
*Optimal dilutions/concentrations should be determined by the researcher.
|Predicted Target Size||67 kDa (note)
|Positive Controls||SK-N-SH , SK-N-AS , mouse brain , rat brain , human GAD1-transfected 293T|
(Please refer to the vial label for the specific concentration)|
|Purification||Purified by antigen-affinity chromatography.|
|Full Name||glutamate decarboxylase 1 (brain, 67kDa)|
|Product Description||Rabbit Polyclonal antibody to GAD67 (glutamate decarboxylase 1 (brain, 67kDa))|
|Background||This gene encodes one of several forms of glutamic acid decarboxylase, identified as a major autoantigen in insulin-dependent diabetes. The enzyme encoded is responsible for catalyzing the production of gamma-aminobutyric acid from L-glutamic acid. A pathogenic role for this enzyme has been identified in the human pancreas since it has been identified as an autoantigen and an autoreactive T cell target in insulin-dependent diabetes. This gene may also play a role in the stiff man syndrome. Deficiency in this enzyme has been shown to lead to pyridoxine dependency with seizures. Alternative splicing of this gene results in two products, the predominant 67-kD form and a less-frequent 25-kD form. [provided by RefSeq]|
|Synonyms||FLJ45882 antibody, GAD antibody, SCP antibody, GAD1 antibody, GAD-67 antibody, glutamate decarboxylase 67 kDa isoform antibody, glutamate decarboxylase 1 antibody, 67 kDa glutamic acid decarboxylase antibody, glutamate decarboxylase 1 (brain, 67kDa) antibody|
|Immunogen||Recombinant protein encompassing a sequence within the N-terminus region of human GAD67. The exact sequence is proprietary.|
|Species Reactivity||Human, Mouse, Rat|
|Predicted Cross Reactivity species Predicted cross-reactivity:|
Predicted cross-reactivity is based on sequence homology, with greater than 80% immunogen sequence identity considered positive.
Please note that we are only able to guarantee products to work in applications and species in which they have been tested.
|Dog, Cat, Pig, Chicken, Chimpanzee, Bovine|
|Predict Reactivity Note||Dog (99%), Cat (99%), Pig (99%), Chicken (90%), Chimpanzee (100%), Bovine (98%)|