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GAPDH antibody [12D3H9]

Anti-GAPDH antibody [12D3H9] used in Western Blot (WB). GTX17307

Cat. No. GTX17307

Host

Mouse

Clonality

Monoclonal

Clone Name

12D3H9

Isotype

IgG

Application

WB, ELISA

Reactivity

Human, Mouse, Rat, Rabbit
Package
100 μg ($399)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1 - 2 μg/mL
ELISA Assay dependent
Not tested in other applications.

Calculated MW

36 kDa. ( Note )

Positive Control

HeLa Cell Lysate

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

GAPDH antibody was raised against a 16 amino acid peptide near the carboxy terminus of human GAPDH.The immunogen is located within amino acids 230 - 280 of GAPDH.

Purification

Purified IgG

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

glyceraldehyde-3-phosphate dehydrogenase , G3PD , GAPD , HEL-S-162eP

Cellular Localization

Cytoplasm, cytosol,Nucleus,Membrane,Cytoplasm, cytoskeleton

Background

The product of this gene catalyzes an important energy-yielding step in carbohydrate metabolism, the reversible oxidative phosphorylation of glyceraldehyde-3-phosphate in the presence of inorganic phosphate and nicotinamide adenine dinucleotide (NAD). The enzyme exists as a tetramer of identical chains. Many pseudogenes similar to this locus are present in the human genome. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Feb 2012]

Database

Research Area

DATA IMAGES

Anti-GAPDH antibody [12D3H9] used in Western Blot (WB). GTX17307

GTX17307 WB Image

WB analysis of multiple cell and tissue lysates (Lanes 1-23 : 293, A431, A549, Daudi, HeLa, HepG2, Jurkat, K562, MOLT, 3T3, Raji, Ramos, U937, human brain, mouse brain, rat brain, rabbit brain, human heart, rat heart, human liver, mouse liver, rat liver, rabbit liver) using GTX17307 GAPDH antibody [12D3H9].
Working concentration : 1 μg/ml

REFERENCE

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REVIEW

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SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399