APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 1/500 - 1/2000 |
| 1/200 - 1/1000 |
| 1/200 - 1/1000 |
| 1/10000 |
Not tested in other applications.
Calculated MW
PROPERTIES
Form
Liquid
Buffer
Ascites
Preservative
0.03% Sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Antigen Species
Human
Immunogen
Purified recombinant fragment of human GAPDH expressed in E. Coli.
Purification
Unpurified
Conjugation
Unconjugated
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
glyceraldehyde-3-phosphate dehydrogenase , G3PD , GAPD , HEL-S-162eP
Cellular Localization
Cytoplasm, cytosol,Nucleus,Membrane,Cytoplasm, cytoskeleton
Background
This gene encodes a member of the glyceraldehyde-3-phosphate dehydrogenase protein family. The encoded protein has been identified as a moonlighting protein based on its ability to perform mechanistically distinct functions. The product of this gene catalyzes an important energy-yielding step in carbohydrate metabolism, the reversible oxidative phosphorylation of glyceraldehyde-3-phosphate in the presence of inorganic phosphate and nicotinamide adenine dinucleotide (NAD). The encoded protein has additionally been identified to have uracil DNA glycosylase activity in the nucleus. Also, this protein contains a peptide that has antimicrobial activity against E. coli, P. aeruginosa, and C. albicans. Studies of a similar protein in mouse have assigned a variety of additional functions including nitrosylation of nuclear proteins, the regulation of mRNA stability, and acting as a transferrin receptor on the cell surface of macrophage. Many pseudogenes similar to this locus are present in the human genome. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Nov 2014]
Database
Research Area
DATA IMAGES
![Anti-GAPDH antibody [1A10] used in Western Blot (WB). GTX83245](/templates/default/js/lightbox/images/loading.gif "Anti-GAPDH antibody [1A10] used in Western Blot (WB). GTX83245")
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GTX83245 WB Image
WB analysis of HeLa (1), A549 (2), A431 (3), MCF-7 (4), K562 (5), Jurkat (6), HL60 (7), SKN-SH (8) and SKBR-3 (9) cell lysate using GTX83245 GAPDH antibody [1A10].
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GTX83245 IHC-P Image
IHC-P analysis of human breast carcinoma (left) and kidney carcinoma (right) using GTX83245 GAPDH antibody [1A10].
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GTX83245 ICC/IF Image
ICC/IF analysis of methanol-fixed HepG2 (left) and HeLa (right) cells using GTX83245 GAPDH antibody [1A10].
Green : GAPDH
Blue: DRAQ5 fluorescent DNA dye
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REFERENCE
There are currently no references for GAPDH antibody [1A10] (GTX83245). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for GAPDH antibody [1A10] (GTX83245). Be the first to share your experience with this product.
![Anti-GAPDH antibody [1A10] used in Western Blot (WB). GTX83245](/upload/website/prouct_img/small/GTX83245/GTX83245_20170912_WB_w_23061322_915.webp "Anti-GAPDH antibody [1A10] used in Western Blot (WB). GTX83245")
![Anti-GAPDH antibody [1A10] used in IHC (Paraffin sections) (IHC-P). GTX83245](/upload/website/prouct_img/small/GTX83245/GTX83245_20170912_IHC-P_w_23061322_641.webp "Anti-GAPDH antibody [1A10] used in IHC (Paraffin sections) (IHC-P). GTX83245")
![Anti-GAPDH antibody [1A10] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX83245](/upload/website/prouct_img/small/GTX83245/GTX83245_20170912_ICCIF_w_23061322_576.webp "Anti-GAPDH antibody [1A10] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX83245")
![Anti-GAPDH antibody [1A10] used in Western Blot (WB). GTX83245](/upload/website/prouct_img/normal/GTX83245/GTX83245_20170912_WB_w_23061322_915.webp "Anti-GAPDH antibody [1A10] used in Western Blot (WB). GTX83245")
![Anti-GAPDH antibody [1A10] used in IHC (Paraffin sections) (IHC-P). GTX83245](/upload/website/prouct_img/normal/GTX83245/GTX83245_20170912_IHC-P_w_23061322_641.webp "Anti-GAPDH antibody [1A10] used in IHC (Paraffin sections) (IHC-P). GTX83245")
![Anti-GAPDH antibody [1A10] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX83245](/upload/website/prouct_img/normal/GTX83245/GTX83245_20170912_ICCIF_w_23061322_576.webp "Anti-GAPDH antibody [1A10] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX83245")