GAPDH antibody [GT239]
|Applications||ICC/IF, IHC-P, WB|
Not tested in other applications.
|IHC (Formalin-fixed paraffin-embedded sections)||1:100-1:1000*
*Optimal dilutions/concentrations should be determined by the researcher.
|Predicted Target Size||36 kDa (note)
|Positive Controls||293T , NIH-3T3 , PC-12 , Jurkat , Raji , A431 , HeLa , HepG2 , H1299 , HCT116 , MCF-7 , NT2D1 , PC-3 , U87-MG , yeast , mouse brain , rat brain , Drosophila , **SHSY-5Y , *KATOIII , *MKN45 , E. coli M15|
(Please refer to the vial label for the specific concentration)|
|Purification||Affinity purified by Protein G.|
|Full Name||glyceraldehyde-3-phosphate dehydrogenase|
|Product Description||Mouse Monoclonal antibody [GT239] to GAPDH (glyceraldehyde-3-phosphate dehydrogenase)|
|Background||The product of this gene catalyzes an important energy-yielding step in carbohydrate metabolism, the reversible oxidative phosphorylation of glyceraldehyde-3-phosphate in the presence of inorganic phosphate and nicotinamide adenine dinucleotide (NAD). The enzyme exists as a tetramer of identical chains. Many pseudogenes similar to this locus are present in the human genome. [provided by RefSeq]|
|Synonyms||G3PD antibody, GAPD antibody, MGC88685 antibody, GAPDH antibody, glyceraldehyde-3-phosphate dehydrogenase antibody, aging-associated gene 9 protein antibody, glyceraldehyde 3-phosphate dehydrogenase antibody, peptidyl-cysteine S-nitrosylase GAPDH antibody|
|Cellular Localization||Cytoplasm , perinuclear region , Membrane |
|Immunogen||Recombinant protein encompassing a sequence within the center region of human GAPDH. The exact sequence is proprietary.|
|Species Reactivity||Human, Mouse, Drosophila, Monkey, Rat, Zebrafish, Yeast, E. coli, Hamster|
|Predicted Cross Reactivity species Predicted cross-reactivity:|
Predicted cross-reactivity is based on sequence homology, with greater than 80% immunogen sequence identity considered positive.
Please note that we are only able to guarantee products to work in applications and species in which they have been tested.
|Xenopus laevis, Dog, Cat, Pig, Rabbit, Chicken, Sheep, Rhesus Monkey, Bovine, Guinea pig|
|Predict Reactivity Note||Xenopus laevis (85%), Dog (95%), Cat (96%), Pig (95%), Rabbit (94%), Chicken (92%), Sheep (94%), Rhesus Monkey (99%), Bovine (94%), Guinea pig (94%)|