|Applications||ICC/IF, IHC-P, IP, WB, IHC-R, IB|
Not tested in other applications.
|IHC (Formalin-fixed paraffin-embedded sections)||1:100-1:1000*
| IHC (Resin sections)||Assay-dependent dilution
| Immunoblotting||Assay-dependent dilution
*Optimal dilutions/concentrations should be determined by the researcher.
|Predicted Target Size||36 kDa (note)
|Positive Controls||293T , A431 , HeLa , HepG2 , E. coli M15 , Neuro 2A , C8D30 , NIH-3T3 , Raw264.7 , C2C12 , PC-12 , Rat2 , *SH-SY5Y , *A549 , *U2OS , *H9C2 , *A7R5 , *mouse skin tissue|
(Please refer to the vial label for the specific concentration)|
|Purification||Purified by antigen-affinity chromatography.|
|Full Name||glyceraldehyde-3-phosphate dehydrogenase|
|Product Description||Rabbit Polyclonal antibody to GAPDH (glyceraldehyde-3-phosphate dehydrogenase)|
|Background||The product of this gene catalyzes an important energy-yielding step in carbohydrate metabolism, the reversible oxidative phosphorylation of glyceraldehyde-3-phosphate in the presence of inorganic phosphate and nicotinamide adenine dinucleotide (NAD). The enzyme exists as a tetramer of identical chains. Many pseudogenes similar to this locus are present in the human genome. [provided by RefSeq]|
|Synonyms||G3PD antibody, GAPD antibody, MGC88685 antibody, GAPDH antibody, glyceraldehyde-3-phosphate dehydrogenase antibody, aging-associated gene 9 protein antibody, glyceraldehyde 3-phosphate dehydrogenase antibody, peptidyl-cysteine S-nitrosylase GAPDH antibody|
|Cellular Localization||Cytoplasm , perinuclear region , Membrane |
|Immunogen||Recombinant protein encompassing a sequence within the center region of human GAPDH. The exact sequence is proprietary.|
|Species Reactivity||Human, Mouse, Dog, Drosophila, Rat, Zebrafish, E. coli, Bovine|
|Predicted Cross Reactivity species Predicted cross-reactivity:|
Predicted cross-reactivity is based on sequence homology, with greater than 80% immunogen sequence identity considered positive.
Please note that we are only able to guarantee products to work in applications and species in which they have been tested.
|Xenopus laevis, Cat, Pig, Rabbit, Chicken, Sheep, Rhesus Monkey, Guinea pig|
|Predict Reactivity Note||Xenopus laevis (85%), Cat (96%), Pig (95%), Rabbit (94%), Chicken (92%), Sheep (94%), Rhesus Monkey (99%), Guinea pig (94%)|