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GAPDH antibody

Anti-GAPDH antibody used in IHC (Paraffin sections) (IHC-P). GTX100118
Anti-GAPDH antibody used in Western Blot (WB). GTX100118
Anti-GAPDH antibody used in Immunoprecipitation (IP). GTX100118
Anti-GAPDH antibody used in Western Blot (WB). GTX100118
Anti-GAPDH antibody used in IHC (Paraffin sections) (IHC-P). GTX100118
Anti-GAPDH antibody used in IHC (Paraffin sections) (IHC-P). GTX100118
Anti-GAPDH antibody used in IHC (Paraffin sections) (IHC-P). GTX100118
Anti-GAPDH antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX100118
Anti-GAPDH antibody used in IHC (Paraffin sections) (IHC-P). GTX100118
Anti-GAPDH antibody used in Western Blot (WB). GTX100118
Anti-GAPDH antibody used in IHC (Paraffin sections) (IHC-P). GTX100118
Anti-GAPDH antibody used in Western Blot (WB). GTX100118
Anti-GAPDH antibody used in Western Blot (WB). GTX100118
Anti-GAPDH antibody used in Western Blot (WB). GTX100118

Cat. No. GTX100118

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, ICC/IF, IHC-P, IP

Reactivity

Human, Mouse, Rat, Zebrafish, Yeast, Rabbit, Drosophila, Bovine, Dog, Hamster, Chicken, Pig, Monkey, Caenorhabditis elegans, E. coli, Mosquito, Nematode, Pika, Plant, Fish, Bacteria, Insect, milkfish
Package
100 μl ($399),
25 μl ($169)
PRODUCT

Summary

GAPDH antibody recognizes glyceraldehyde-3-phosphate dehydrogenase (GAPDH) protein, a glycolytic enzyme of ~37 kDa that forms a tetramer. Because of its widespread expression, GAPDH is commonly used as an internal control for various protein assays, particularly western blot, to validate both the amount and quality of the loaded lysate samples.

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:5000-1:100000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
IP 1:100-1:500
Not tested in other applications.

Calculated MW

36 kDa. ( Note )

Positive Control

PC-12 , Rat2 , Neuro 2A , C8D30 , NIH-3T3 , Raw264.7 , C2C12 , E.coli M15

Predict Reactivity

Sheep, Cat, Guinea pig, Xenopus tropicalis, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.11 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human GAPDH. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1080976

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

glyceraldehyde-3-phosphate dehydrogenase , G3PD , GAPD , HEL-S-162eP

Cellular Localization

Cytoplasm , perinuclear region , Membrane

Background

The product of this gene catalyzes an important energy-yielding step in carbohydrate metabolism, the reversible oxidative phosphorylation of glyceraldehyde-3-phosphate in the presence of inorganic phosphate and nicotinamide adenine dinucleotide (NAD). The enzyme exists as a tetramer of identical chains. Many pseudogenes similar to this locus are present in the human genome. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-GAPDH antibody used in IHC (Paraffin sections) (IHC-P). GTX100118

GTX100118 IHC-P Image

GAPDH antibody detects GAPDH protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded human duodenum.
GAPDH stained by GAPDH antibody (GTX100118) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-GAPDH antibody used in Western Blot (WB). GTX100118

GTX100118 WB Image

Whole cell extract (30 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with GAPDH antibody (GTX100118) diluted at 1:5000.

Anti-GAPDH antibody used in Immunoprecipitation (IP). GTX100118

GTX100118 IP Image

Immunoprecipitation of GAPDH protein from 293T whole cell extracts using 5 μg of GAPDH antibody (GTX100118).
Western blot analysis was performed using GAPDH antibody (GTX100118).
EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.

Anti-GAPDH antibody used in Western Blot (WB). GTX100118

GTX100118 WB Image

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with GAPDH antibody (GTX100118) diluted at 1:6000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-GAPDH antibody used in IHC (Paraffin sections) (IHC-P). GTX100118

GTX100118 IHC-P Image

GAPDH antibody detects GAPDH protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded mouse intestine.
GAPDH stained by GAPDH antibody (GTX100118) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-GAPDH antibody used in IHC (Paraffin sections) (IHC-P). GTX100118

GTX100118 IHC-P Image

GAPDH antibody detects GAPDH protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded rat testis.
GAPDH stained by GAPDH antibody (GTX100118) diluted at 1:1000.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-GAPDH antibody used in IHC (Paraffin sections) (IHC-P). GTX100118

GTX100118 IHC-P Image

GAPDH antibody detects GAPDH protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded mouse liver.
GAPDH stained by GAPDH antibody (GTX100118) diluted at 1:1000.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-GAPDH antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX100118

GTX100118 ICC/IF Image

GAPDH antibody detects GAPDH protein at endoplasmic reticulum, cytoplasm and nucleus by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: GAPDH stained by GAPDH antibody (GTX100118) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

Anti-GAPDH antibody used in IHC (Paraffin sections) (IHC-P). GTX100118

GTX100118 IHC-P Image

GAPDH antibody detects GAPDH protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded mouse duodenum.
GAPDH stained by GAPDH antibody (GTX100118) diluted at 1:1000.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-GAPDH antibody used in Western Blot (WB). GTX100118

GTX100118 WB Image

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with GAPDH antibody (GTX100118) diluted at 1:100000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-GAPDH antibody used in IHC (Paraffin sections) (IHC-P). GTX100118

GTX100118 IHC-P Image

GAPDH antibody detects GAPDH protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded rat liver.
GAPDH stained by GAPDH antibody (GTX100118) diluted at 1:1000.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-GAPDH antibody used in Western Blot (WB). GTX100118

GTX100118 WB Image

Various whole cell extracts were separated by 10% SDS-PAGE, and the membrane was blotted with GAPDH antibody (GTX100118) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-GAPDH antibody used in Western Blot (WB). GTX100118

GTX100118 WB Image

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with GAPDH antibody (GTX100118) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-GAPDH antibody used in Western Blot (WB). GTX100118

GTX100118 WB Image

Whole cell extract (50 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with GAPDH antibody (GTX100118) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE

REVIEW

GAPDH antibody Cat. No. GTX100118

Rating ( Average 5 based on 192 users reviews)
Application
Western Blot(WB) ( Average 5 based on 191 users reviews)
Immunocytochemistry/ Immunofluorescence(ICC/IF) ( Average 5 based on 1 users reviews)
Immunoprecipitation(IP) ( Average 5 based on 1 users reviews)
Date : Anonymous submitted on 22-Sep-2023
Score :
Application Tested : WB
Sample Species : Hu, Ms
Sample : HEK293T cells, NIH3T3 cells
Amount used : 30 μg
Blocking : 5% nonfat-skim milk in TBST, 25°C, 1Hr
Primary Antibody : 1:5000, 4°C, 17Hr
Date : Anonymous submitted on 24-Jul-2023
Score :
Application Tested : WB
Sample Species : Dm
Figure Note : The data was peer reviewed and published in the 2022 in Cell Death Dis. PMID: 36435842
Date : Anonymous submitted on 24-Jul-2023
Score :
Application Tested : WB
Sample Species : Hu
Figure Note : The data was peer reviewed and published in the 2022 in Cell Death Dis. PMID: 35688824
Date : Anonymous submitted on 24-Jul-2023
Score :
Application Tested : WB
Sample Species : Hu
Figure Note : The data was peer reviewed and published in the 2021 in Cancer Drug Resist. PMID: 35582384
Date : Anonymous submitted on 24-Jul-2023
Score :
Application Tested : WB
Sample Species : Mosq
Figure Note : The data was peer reviewed and published in the 2022 in Front Microbiol. PMID: 35572621
Date : Anonymous submitted on 24-Jul-2023
Score :
Application Tested : WB
Sample Species : Mosq
Figure Note : The data was peer reviewed and published in the 2022 in Front Microbiol. PMID: 35572621
Date : Anonymous submitted on 24-Jul-2023
Score :
Application Tested : WB
Sample Species : Mosq
Figure Note : The data was peer reviewed and published in the 2022 in Front Microbiol. PMID: 35572621
Date : Anonymous submitted on 24-Jul-2023
Score :
Application Tested : WB
Sample Species : Hu
Figure Note : The data was peer reviewed and published in the 2022 in Front Pharmacol. PMID: 35517780
Date : Anonymous submitted on 24-Jul-2023
Score :
Application Tested : WB
Sample Species : Hu
Figure Note : The data was peer reviewed and published in the 2022 in Front Pharmacol. PMID: 35517780
Date : Anonymous submitted on 24-Jul-2023
Score :
Application Tested : WB
Sample Species : Hu
Figure Note : The data was peer reviewed and published in the 2022 in Front Pharmacol. PMID: 35517780
Date : Anonymous submitted on 24-Jul-2023
Score :
Application Tested : WB
Sample Species : Hu
Figure Note : The data was peer reviewed and published in the 2022 in Front Pharmacol. PMID: 35517780
Date : Anonymous submitted on 24-Jul-2023
Score :
Application Tested : WB
Sample Species : Hu
Figure Note : The data was peer reviewed and published in the 2022 in Pharmaceutics. PMID: 35456673
Date : Anonymous submitted on 24-Jul-2023
Score :
Application Tested : WB
Sample Species : Hu
Figure Note : The data was peer reviewed and published in the 2022 in Biomedicines. PMID: 35203455
Date : Anonymous submitted on 24-Jul-2023
Score :
Application Tested : WB
Sample Species : Rat
Figure Note : The data was peer reviewed and published in the 2021 in Stem Cell Res Ther. PMID: 34020702
Date : Anonymous submitted on 24-Jul-2023
Score :
Application Tested : WB
Sample Species : Hu
Figure Note : The data was peer reviewed and published in the 2021 in Cancer Cell Int. PMID: 33926470
Date : Anonymous submitted on 24-Jul-2023
Score :
Application Tested : WB
Sample Species : Hu
Figure Note : The data was peer reviewed and published in the 2021 in Cancer Cell Int. PMID: 33926470
Date : Anonymous submitted on 24-Jul-2023
Score :
Application Tested : WB
Sample Species : Ms
Figure Note : The data was peer reviewed and published in the 2021 in Eur Psychiatry. PMID: 33413711
Date : Anonymous submitted on 24-Jul-2023
Score :
Application Tested : WB
Sample Species : Hu
Figure Note : The data was peer reviewed and published in the 2020 in Sci Rep. PMID: 32424165
Date : Anonymous submitted on 24-Jul-2023
Score :
Application Tested : WB
Sample Species : Dm
Figure Note : The data was peer reviewed and published in the 2019 in Oncogene. PMID: 30792539
Date : Anonymous submitted on 24-Jul-2023
Score :
Application Tested : WB
Sample Species : Dm
Figure Note : The data was peer reviewed and published in the 2019 in Oncogene. PMID: 30792539
Date : Anonymous submitted on 24-Jul-2023
Score :
Application Tested : WB
Sample Species : Dm
Figure Note : The data was peer reviewed and published in the 2019 in Oncogene. PMID: 30792539
Date : Anonymous submitted on 12-Jul-2023
Score :
Application Tested : WB
Sample Species : Ms
Sample : mouse liver tissue lysate (ICR)
Amount used : 40μg
Blocking : 5% non-fat milk in TBST, 25°C, 1Hr
Primary Antibody : Undiluted, 4°C, >12Hr
Date : Anonymous submitted on 03-Oct-2022
Score :
Application Tested : WB
Sample Species : Ms
Figure Note : The data was peer reviewed and published in the 2022 in Int J Mol Sci. PMID: 35328622
Date : Anonymous submitted on 03-Oct-2022
Score :
Application Tested : WB
Sample Species : Hu
Figure Note : The data was peer reviewed and published in the 2022 in Front Cell Dev Biol. PMID: 35309907
Date : Anonymous submitted on 03-Oct-2022
Score :
Application Tested : WB
Sample Species : Hu
Figure Note : The data was peer reviewed and published in the 2022 in FEBS Open Bio. PMID: 34856073
Date : Anonymous submitted on 03-Oct-2022
Score :
Application Tested : WB
Sample Species : Hu
Figure Note : The data was peer reviewed and published in the 2020 in Sci Rep. PMID: 33177556
Date : Anonymous submitted on 03-Oct-2022
Score :
Application Tested : WB
Sample Species : Hu
Figure Note : The data was peer reviewed and published in the 2019 in Cell Div. PMID: 31452676
Date : Anonymous submitted on 03-Oct-2022
Score :
Application Tested : WB
Sample Species : Hu
Figure Note : The data was peer reviewed and published in the 2019 in Cell Div. PMID: 31452676
Date : Anonymous submitted on 03-Oct-2022
Score :
Application Tested : WB
Sample Species : Hu
Figure Note : The data was peer reviewed and published in the 2019 in Cell Div. PMID: 31452676
Date : Anonymous submitted on 03-Oct-2022
Score :
Application Tested : WB
Sample Species : Hu
Figure Note : The data was peer reviewed and published in the 2014 in Alzheimers Res Ther. PMID: 25478031
Date : Anonymous submitted on 10-Aug-2021
Score :
Application Tested : WB
Sample Species : Hu
Figure Note : The data was peer reviewed and published in the journal Stem Cell Res Ther in 2018.PMID: 29751772
Date : Anonymous submitted on 10-Aug-2021
Score :
Application Tested : WB
Sample Species : Hu
Figure Note : The data was peer reviewed and published in the journal PLoS One in 2016.PMID: 27023634
Date : Anonymous submitted on 10-Aug-2021
Score :
Application Tested : WB
Sample Species : Hu
Figure Note : The data was peer reviewed and published in the journal PLoS One in 2016.PMID: 27023634
Date : Anonymous submitted on 10-Aug-2021
Score :
Application Tested : WB
Sample Species : Hu
Figure Note : The data was peer reviewed and published in the journal PLoS One in 2016.PMID: 27023634
Date : Anonymous submitted on 10-Aug-2021
Score :
Application Tested : WB
Sample Species : Hu
Figure Note : The data was peer reviewed and published in the journal PLoS One in 2016.PMID: 27023634
Date : Anonymous submitted on 10-Aug-2021
Score :
Application Tested : WB
Sample Species : Hu
Figure Note : The data was peer reviewed and published in the journal Oncotarget in 2015.PMID: 25797272
Date : Anonymous submitted on 10-Aug-2021
Score :
Application Tested : WB
Sample Species : Hu
Figure Note : The data was peer reviewed and published in the journal Front Immunol in 2020.PMID: 32210961
Date : Anonymous submitted on 10-Aug-2021
Score :
Application Tested : WB
Sample Species : Hu
Figure Note : The data was peer reviewed and published in the journal Sci Rep in 2017.PMID: 28098200
Date : Anonymous submitted on 10-Aug-2021
Score :
Application Tested : WB
Sample Species : Hu
Figure Note : The data was peer reviewed and published in the journal PLoS Biol in 2015.PMID: 26406915
Date : Anonymous submitted on 10-Aug-2021
Score :
Application Tested : WB
Sample Species : Hu
Figure Note : The data was peer reviewed and published in the journal Front Immunol in 2020.PMID: 32210961
Date : Anonymous submitted on 10-Aug-2021
Score :
Application Tested : WB
Sample Species : Hu
Figure Note : The data was peer reviewed and published in the journal Front Aging Neurosci in 2014.PMID: 25136316
Date : Anonymous submitted on 10-Aug-2021
Score :
Application Tested : WB
Sample Species : Hu
Figure Note : The data was peer reviewed and published in the journal Mol Oncol in 2020.PMID: 32037723
Date : Anonymous submitted on 10-Aug-2021
Score :
Application Tested : WB
Sample Species : Ms
Figure Note : The data was peer reviewed and published in the journal PLoS Biol in 2015.PMID: 26406915
Date : Anonymous submitted on 10-Aug-2021
Score :
Application Tested : WB
Sample Species : Hu
Figure Note : The data was peer reviewed and published in the journal J Virol in 2016.PMID: 27489281
Date : Anonymous submitted on 10-Aug-2021
Score :
Application Tested : WB
Sample Species : Ms
Figure Note : The data was peer reviewed and published in the journal Oncotarget in 2016.PMID: 27564110
Date : Anonymous submitted on 10-Aug-2021
Score :
Application Tested : WB
Sample Species : Hu, Ms
Figure Note : The data was peer reviewed and published in the journal Stem Cells Transl Med in 2020.PMID: 31943851
Date : Anonymous submitted on 10-Aug-2021
Score :
Application Tested : WB
Sample Species : Hu
Figure Note : The data was peer reviewed and published in the journal Stem Cell Res Ther in 2018.PMID: 29751772
Date : Anonymous submitted on 10-Aug-2021
Score :
Application Tested : WB
Sample Species : Ms
Figure Note : The data was peer reviewed and published in the journal Nat Commun in 2020.PMID: 32286300
Date : Anonymous submitted on 10-Aug-2021
Score :
Application Tested : WB
Sample Species : Hu
Figure Note : The data was peer reviewed and published in the journal Stem Cell Res Ther in 2018.PMID: 29751772
Date : Anonymous submitted on 10-Aug-2021
Score :
Application Tested : WB
Sample Species : Hu
Figure Note : The data was peer reviewed and published in the journal Stem Cell Res Ther in 2018.PMID: 29751772
Date : Anonymous submitted on 10-Aug-2021
Score :
Application Tested : WB
Sample Species : Hu
Figure Note : The data was peer reviewed and published in the journal PLoS One in 2016.PMID: 27383761
Date : Anonymous submitted on 10-Aug-2021
Score :
Application Tested : WB
Sample Species : Ms
Figure Note : The data was peer reviewed and published in the journal PLoS Biol in 2015.PMID: 26406915
Date : Anonymous submitted on 10-Aug-2021
Score :
Application Tested : WB
Sample Species : Hu
Figure Note : The data was peer reviewed and published in the journal BMC Cancer in 2018.PMID: 30594165
Date : Anonymous submitted on 10-Aug-2021
Score :
Application Tested : WB
Sample Species : Hu
Figure Note : The data was peer reviewed and published in the journal Nat Commun in 2020.PMID: 32286300
Date : Anonymous submitted on 10-Aug-2021
Score :
Application Tested : WB
Sample Species : Hu
Figure Note : The data was peer reviewed and published in the journal Sci Rep in 2017.PMID: 28098200
Date : Anonymous submitted on 10-Aug-2021
Score :
Application Tested : WB
Sample Species : Hu
Figure Note : The data was peer reviewed and published in the journal Int J Mol Sci in 2020.PMID: 32545770
Date : Anonymous submitted on 10-Aug-2021
Score :
Application Tested : WB
Sample Species : Hu
Figure Note : The data was peer reviewed and published in the journal Int J Mol Sci in 2020.PMID: 32545770
Date : Anonymous submitted on 10-Aug-2021
Score :
Application Tested : WB
Sample Species : Hu
Figure Note : The data was peer reviewed and published in the journal Int J Mol Sci in 2020.PMID: 32098449
Date : Anonymous submitted on 10-Aug-2021
Score :
Application Tested : WB
Sample Species : Hu
Figure Note : The data was peer reviewed and published in the journal PLoS One in 2019.PMID: 30893332
Date : Anonymous submitted on 10-Aug-2021
Score :
Application Tested : WB
Sample Species : Hu
Figure Note : The data was peer reviewed and published in the journal Sci Rep in 2020.PMID: 32427884
Date : Anonymous submitted on 10-Aug-2021
Score :
Application Tested : WB
Sample Species : Hu
Figure Note : The data was peer reviewed and published in the journal PLoS One in 2016.PMID: 27023634
Date : Anonymous submitted on 10-Aug-2021
Score :
Application Tested : WB
Sample Species : Hu
Figure Note : The data was peer reviewed and published in the journal PLoS One in 2016.PMID: 27023634
Date : Anonymous submitted on 10-Aug-2021
Score :
Application Tested : WB
Sample Species : Hu
Figure Note : The data was peer reviewed and published in the journal Sci Rep in 2017.PMID: 28098200
Date : Anonymous submitted on 10-Aug-2021
Score :
Application Tested : WB
Sample Species : Zfsh
Figure Note : The data was peer reviewed and published in the journal Cell Death Dis in 2019.PMID: 31427568
Date : Anonymous submitted on 10-Aug-2021
Score :
Application Tested : WB
Sample Species : Hu
Figure Note : The data was peer reviewed and published in the journal Front Immunol in 2020.PMID: 32210961
Date : Anonymous submitted on 10-Aug-2021
Score :
Application Tested : WB
Sample Species : Hu
Figure Note : The data was peer reviewed and published in the journal Int J Mol Sci in 2020.PMID: 32545770
Date : Anonymous submitted on 10-Aug-2021
Score :
Application Tested : WB
Sample Species : Hu
Figure Note : The data was peer reviewed and published in the journal Oncotarget in 2015.PMID: 25797272
Date : Anonymous submitted on 10-Aug-2021
Score :
Application Tested : WB
Sample Species : Hu
Figure Note : The data was peer reviewed and published in the journal Front Immunol in 2020.PMID: 32210961
Date : Anonymous submitted on 10-Aug-2021
Score :
Application Tested : WB
Sample Species : Hu
Figure Note : The data was peer reviewed and published in the journal Stem Cells Transl Med in 2020.PMID: 31943851
Date : Anonymous submitted on 10-Aug-2021
Score :
Application Tested : WB
Sample Species : Hu
Figure Note : The data was peer reviewed and published in the journal Sci Rep in 2017.PMID: 28098200
Date : Anonymous submitted on 10-Aug-2021
Score :
Application Tested : WB
Sample Species : Hu
Figure Note : The data was peer reviewed and published in the journal Sci Rep in 2017.PMID: 28098200
Date : Anonymous submitted on 30-Mar-2021
Score :
Application Tested : WB
Sample Species : Hu
Sample : OC-3-VGH cells
Amount used : 35 μg
Blocking : 5% non-fat milk in TBST, 4°C, 20Hr
Primary Antibody : 1:5000, 4°C, 20Hr
Date : Anonymous submitted on 18-Mar-2021
Score :
Application Tested : WB
Sample Species : milkfish
Figure Note : The data was peer reviewed and published in the journal Front Physiol in 2016. PMID: 27965586
Date : Anonymous submitted on 18-Mar-2021
Score :
Application Tested : WB
Sample Species : milkfish
Figure Note : The data was peer reviewed and published in the journal Front Physiol in 2016. PMID: 27965586
Date : Anonymous submitted on 18-Mar-2021
Score :
Application Tested : WB
Sample Species : Mk
Figure Note : The data was peer reviewed and published in the journal PLoS One in 2016. PMID: 26863132
Date : Anonymous submitted on 18-Mar-2021
Score :
Application Tested : WB
Sample Species : Rat
Figure Note : The data was peer reviewed and published in the journal Physiol Rep in 2016. PMID: 27482075
Date : Anonymous submitted on 18-Mar-2021
Score :
Application Tested : WB
Sample Species : Rat
Figure Note : The data was peer reviewed and published in the journal PLoS One in 2015. PMID: 25938237
Date : Anonymous submitted on 18-Mar-2021
Score :
Application Tested : WB
Sample Species : Rat
Figure Note : The data was peer reviewed and published in the journal PLoS One in 2015. PMID: 25938237
Date : Anonymous submitted on 18-Mar-2021
Score :
Application Tested : WB
Sample Species : Rat
Figure Note : The data was peer reviewed and published in the journal PLoS One in 2015. PMID: 25938237
Date : Anonymous submitted on 18-Mar-2021
Score :
Application Tested : WB
Sample Species : Rat
Figure Note : The data was peer reviewed and published in the journal J Biomed Sci in 2013. PMID: 24359494
Date : Anonymous submitted on 18-Mar-2021
Score :
Application Tested : WB
Sample Species : Rat
Figure Note : The data was peer reviewed and published in the journal J Biomed Sci in 2013. PMID: 24359494
Date : Anonymous submitted on 18-Mar-2021
Score :
Application Tested : WB
Sample Species : Rat
Figure Note : The data was peer reviewed and published in the journal Evid Based Complement Alternat Med in 2013. PMID: 23573126
Date : Anonymous submitted on 18-Mar-2021
Score :
Application Tested : WB
Sample Species : Rat
Figure Note : The data was peer reviewed and published in the journal Evid Based Complement Alternat Med in 2013. PMID: 23573126
Date : Anonymous submitted on 18-Mar-2021
Score :
Application Tested : WB
Sample Species : Ms
Figure Note : The data was peer reviewed and published in the journal PLoS Genet in 2015. PMID: 25760041
Date : Anonymous submitted on 18-Mar-2021
Score :
Application Tested : WB
Sample Species : Ms
Figure Note : The data was peer reviewed and published in the journal Dis Model Mech in 2014. PMID: 24271779
Date : Anonymous submitted on 18-Mar-2021
Score :
Application Tested : WB
Sample Species : Ms
Figure Note : The data was peer reviewed and published in the journal Dis Model Mech in 2014. PMID: 24271779
Date : Anonymous submitted on 18-Mar-2021
Score :
Application Tested : WB
Sample Species : Ms
Figure Note : The data was peer reviewed and published in the journal Cell Discov in 2015. PMID: 27462424
Date : Anonymous submitted on 18-Mar-2021
Score :
Application Tested : WB
Sample Species : Ms
Figure Note : The data was peer reviewed and published in the journal Dis Model Mech in 2014. PMID: 24271779
Date : Anonymous submitted on 18-Mar-2021
Score :
Application Tested : WB
Sample Species : Ms
Figure Note : The data was peer reviewed and published in the journal PLoS One in 2015. PMID: 25799508
Date : Anonymous submitted on 18-Mar-2021
Score :
Application Tested : WB
Sample Species : Ms
Figure Note : The data was peer reviewed and published in the journal Cell Discov in 2015. PMID: 27462424
Date : Anonymous submitted on 18-Mar-2021
Score :
Application Tested : WB
Sample Species : Ms
Figure Note : The data was peer reviewed and published in the journal J Biomed Sci in 2014. PMID: 25200553
Date : Anonymous submitted on 18-Mar-2021
Score :
Application Tested : WB
Sample Species : Ms
Figure Note : The data was peer reviewed and published in the journal J Biomed Sci in 2014. PMID: 25200553
Date : Anonymous submitted on 18-Mar-2021
Score :
Application Tested : WB
Sample Species : Ms
Figure Note : The data was peer reviewed and published in the journal J Biomed Sci in 2014. PMID: 25200553
Date : Anonymous submitted on 18-Mar-2021
Score :
Application Tested : WB
Sample Species : Ms
Figure Note : The data was peer reviewed and published in the journal PLoS One in 2015. PMID: 26305546
Date : Anonymous submitted on 18-Mar-2021
Score :
Application Tested : WB
Sample Species : Hu, Ms
Figure Note : The data was peer reviewed and published in the journal Evid Based Complement Alternat Med in 2013. PMID: 24282433
Date : Anonymous submitted on 18-Mar-2021
Score :
Application Tested : WB
Sample Species : Hu
Figure Note : The data was peer reviewed and published in the journal PLoS One in 2015. PMID: 26360058
Date : Anonymous submitted on 18-Mar-2021
Score :
Application Tested : WB
Sample Species : Hu
Figure Note : The data was peer reviewed and published in the journal PLoS One in 2015. PMID: 26360058
Date : Anonymous submitted on 18-Mar-2021
Score :
Application Tested : WB
Sample Species : Hu
Figure Note : The data was peer reviewed and published in the journal PLoS One in 2015. PMID: 26360058
Date : Anonymous submitted on 18-Mar-2021
Score :
Application Tested : WB
Sample Species : Hu
Figure Note : The data was peer reviewed and published in the journal PLoS One in 2015. PMID: 26360058
Date : Anonymous submitted on 18-Mar-2021
Score :
Application Tested : WB
Sample Species : Hu
Figure Note : The data was peer reviewed and published in the journal Evid Based Complement Alternat Med in 2012. PMID: 23024692
Date : Anonymous submitted on 18-Mar-2021
Score :
Application Tested : WB
Sample Species : Hu
Figure Note : The data was peer reviewed and published in the journal PLoS One in 2014. PMID: 24551118
Date : Anonymous submitted on 18-Mar-2021
Score :
Application Tested : WB
Sample Species : Hu
Figure Note : The data was peer reviewed and published in the journal PLoS One in 2014. PMID: 24551118
Date : Anonymous submitted on 18-Mar-2021
Score :
Application Tested : WB
Sample Species : Hu
Figure Note : The data was peer reviewed and published in the journal J Cell Mol Med in 2014. PMID: 24400868
Date : Anonymous submitted on 18-Mar-2021
Score :
Application Tested : WB
Sample Species : Hu
Figure Note : The data was peer reviewed and published in the journal J Cell Mol Med in 2014. PMID: 24400868
Date : Anonymous submitted on 18-Mar-2021
Score :
Application Tested : WB
Sample Species : Hu
Figure Note : The data was peer reviewed and published in the journal J Cell Mol Med in 2014. PMID: 24400868
Date : Anonymous submitted on 18-Mar-2021
Score :
Application Tested : WB
Sample Species : Hu
Figure Note : The data was peer reviewed and published in the journal J Hematol Oncol in 2018. PMID: 29458389
Date : Anonymous submitted on 18-Mar-2021
Score :
Application Tested : WB
Sample Species : Hu
Figure Note : The data was peer reviewed and published in the journal J Cell Mol Med in 2014. PMID: 24400868
Date : Anonymous submitted on 18-Mar-2021
Score :
Application Tested : WB
Sample Species : Hu
Figure Note : The data was peer reviewed and published in the journal Cell Death Dis in 2013. PMID: 23788038
Date : Anonymous submitted on 18-Mar-2021
Score :
Application Tested : WB
Sample Species : Hu
Figure Note : The data was peer reviewed and published in the journal Cell Death Dis in 2013. PMID: 23788038
Date : Anonymous submitted on 18-Mar-2021
Score :
Application Tested : WB
Sample Species : Hu
Figure Note : The data was peer reviewed and published in the journal Breast Cancer Res in 2017. PMID: 29258605
Date : Anonymous submitted on 18-Mar-2021
Score :
Application Tested : WB
Sample Species : Rat
Figure Note : The data was peer reviewed and published in the journal Biomolecules in 2019. PMID: 31443530
Date : Anonymous submitted on 18-Mar-2021
Score :
Application Tested : WB
Sample Species : Rat
Figure Note : The data was peer reviewed and published in the journal Biomolecules in 2019. PMID: 31443530
Date : Anonymous submitted on 18-Mar-2021
Score :
Application Tested : WB
Sample Species : Rat
Figure Note : The data was peer reviewed and published in the journal Biomolecules in 2019. PMID: 31443530
Date : Anonymous submitted on 18-Mar-2021
Score :
Application Tested : WB
Sample Species : Rat
Figure Note : The data was peer reviewed and published in the journal Biomolecules in 2019. PMID: 31443530
Date : Anonymous submitted on 18-Mar-2021
Score :
Application Tested : WB
Sample Species : Rat
Figure Note : The data was peer reviewed and published in the journal Biomolecules in 2019. PMID: 31443530
Date : Anonymous submitted on 18-Mar-2021
Score :
Application Tested : WB
Sample Species : Rat
Figure Note : The data was peer reviewed and published in the journal Biomolecules in 2019. PMID: 31443530
Date : Anonymous submitted on 18-Mar-2021
Score :
Application Tested : WB
Sample Species : Rat
Figure Note : The data was peer reviewed and published in the journal J Int Soc Sports Nutr in 2015. PMID: 25792976
Date : Anonymous submitted on 18-Mar-2021
Score :
Application Tested : WB
Sample Species : Ms
Figure Note : The data was peer reviewed and published in the journal Nat Commun in 2020. PMID: 32358491
Date : Anonymous submitted on 18-Mar-2021
Score :
Application Tested : WB
Sample Species : Ms
Figure Note : The data was peer reviewed and published in the journal Aging Cell in 2020. PMID: 31833196
Date : Anonymous submitted on 18-Mar-2021
Score :
Application Tested : WB
Sample Species : Ms
Figure Note : The data was peer reviewed and published in the journal Front Endocrinol (Lausanne) in 2017. PMID: 29109696
Date : Anonymous submitted on 18-Mar-2021
Score :
Application Tested : WB
Sample Species : Ms
Figure Note : The data was peer reviewed and published in the journal Int J Mol Sci in 2016. PMID: 26938532
Date : Anonymous submitted on 18-Mar-2021
Score :
Application Tested : WB
Sample Species : Ms
Figure Note : The data was peer reviewed and published in the journal Oncogenesis in 2015. PMID: 26690546
Date : Anonymous submitted on 18-Mar-2021
Score :
Application Tested : WB
Sample Species : Ms
Figure Note : The data was peer reviewed and published in the journal Oncogenesis in 2015. PMID: 26690546
Date : Anonymous submitted on 18-Mar-2021
Score :
Application Tested : WB
Sample Species : Ms
Figure Note : The data was peer reviewed and published in the journal Int J Mol Sci in 2016. PMID: 26938532
Date : Anonymous submitted on 18-Mar-2021
Score :
Application Tested : WB
Sample Species : Mosq
Figure Note : The data was peer reviewed and published in the journal PLoS Negl Trop Dis in 2018. PMID: 29505577
Date : Anonymous submitted on 18-Mar-2021
Score :
Application Tested : WB
Sample Species : Mk
Figure Note : The data was peer reviewed and published in the journal mSphere in 2019. PMID: 30814317
Date : Anonymous submitted on 18-Mar-2021
Score :
Application Tested : WB,IP
Sample Species : Hu
Figure Note : The data was peer reviewed and published in the journal J Hematol Oncol in 2019. PMID: 31324208
Date : Anonymous submitted on 18-Mar-2021
Score :
Application Tested : WB
Sample Species : Hu
Figure Note : The data was peer reviewed and published in the journal Front Microbiol in 2019. PMID: 31969874
Date : Anonymous submitted on 18-Mar-2021
Score :
Application Tested : WB
Sample Species : Hu
Figure Note : The data was peer reviewed and published in the journal Cell Adh Migr in 2019. PMID: 31240993
Date : Anonymous submitted on 18-Mar-2021
Score :
Application Tested : WB
Sample Species : Hu
Figure Note : The data was peer reviewed and published in the journal Cell Div in 2019. PMID: 31110557
Date : Anonymous submitted on 18-Mar-2021
Score :
Application Tested : WB
Sample Species : Hu
Figure Note : The data was peer reviewed and published in the journal Cancers (Basel) in 2019. PMID: 30634502
Date : Anonymous submitted on 18-Mar-2021
Score :
Application Tested : WB
Sample Species : Hu
Figure Note : The data was peer reviewed and published in the journal J Immunol Res in 2018. PMID: 30410941
Date : Anonymous submitted on 18-Mar-2021
Score :
Application Tested : WB
Sample Species : Hu
Figure Note : The data was peer reviewed and published in the journal Oncotarget in 2018. PMID: 29899821
Date : Anonymous submitted on 18-Mar-2021
Score :
Application Tested : WB
Sample Species : Hu
Figure Note : The data was peer reviewed and published in the journal Oncotarget in 2018. PMID: 29899821
Date : Anonymous submitted on 18-Mar-2021
Score :
Application Tested : WB
Sample Species : Hu
Figure Note : The data was peer reviewed and published in the journal Oncotarget in 2018. PMID: 29899821
Date : Anonymous submitted on 18-Mar-2021
Score :
Application Tested : WB
Sample Species : Hu
Figure Note : The data was peer reviewed and published in the journal Oncotarget in 2018. PMID: 29899821
Date : Anonymous submitted on 18-Mar-2021
Score :
Application Tested : WB
Sample Species : Hu
Figure Note : The data was peer reviewed and published in the journal Oncotarget in 2018. PMID: 29899821
Date : Anonymous submitted on 18-Mar-2021
Score :
Application Tested : WB
Sample Species : Hu
Figure Note : The data was peer reviewed and published in the journal Oncotarget in 2018. PMID: 29899821
Date : Anonymous submitted on 18-Mar-2021
Score :
Application Tested : WB
Sample Species : Hu
Figure Note : The data was peer reviewed and published in the journal Int J Mol Sci in 2018. PMID: 29518977
Date : Anonymous submitted on 18-Mar-2021
Score :
Application Tested : WB
Sample Species : Hu
Figure Note : The data was peer reviewed and published in the journal J Immunol Res in 2017. PMID: 29423418
Date : Anonymous submitted on 18-Mar-2021
Score :
Application Tested : WB
Sample Species : Hu
Figure Note : The data was peer reviewed and published in the journal Virulence in 2018. PMID: 29338543
Date : Anonymous submitted on 18-Mar-2021
Score :
Application Tested : WB
Sample Species : Hu
Figure Note : The data was peer reviewed and published in the journal Virulence in 2018. PMID: 29338543
Date : Anonymous submitted on 18-Mar-2021
Score :
Application Tested : WB
Sample Species : Hu
Figure Note : The data was peer reviewed and published in the journal Virulence in 2018. PMID: 29338543
Date : Anonymous submitted on 18-Mar-2021
Score :
Application Tested : WB
Sample Species : Hu
Figure Note : The data was peer reviewed and published in the journal Virulence in 2018. PMID: 29338543
Date : Anonymous submitted on 18-Mar-2021
Score :
Application Tested : WB
Sample Species : Hu
Figure Note : The data was peer reviewed and published in the journal Front Endocrinol (Lausanne) in 2017. PMID: 29109696
Date : Anonymous submitted on 18-Mar-2021
Score :
Application Tested : WB
Sample Species : Hu
Figure Note : The data was peer reviewed and published in the journal Oncoscience in 2016. PMID: 28105458
Date : Anonymous submitted on 18-Mar-2021
Score :
Application Tested : WB
Sample Species : Hu
Figure Note : The data was peer reviewed and published in the journal Oncotarget in 2016. PMID: 27191263
Date : Anonymous submitted on 18-Mar-2021
Score :
Application Tested : WB
Sample Species : Hu
Figure Note : The data was peer reviewed and published in the journal Oncotarget in 2016. PMID: 27191263
Date : Anonymous submitted on 18-Mar-2021
Score :
Application Tested : WB
Sample Species : Hu
Figure Note : The data was peer reviewed and published in the journal Oncotarget in 2016. PMID: 27034167
Date : Anonymous submitted on 18-Mar-2021
Score :
Application Tested : WB
Sample Species : Hu
Figure Note : The data was peer reviewed and published in the journal Oncotarget in 2016. PMID: 27034167
Date : Anonymous submitted on 18-Mar-2021
Score :
Application Tested : WB
Sample Species : Hu
Figure Note : The data was peer reviewed and published in the journal Oncotarget in 2016. PMID: 27034167
Date : Anonymous submitted on 18-Mar-2021
Score :
Application Tested : WB
Sample Species : Hu
Figure Note : The data was peer reviewed and published in the journal Oncogenesis in 2015. PMID: 26690546
Date : Anonymous submitted on 18-Mar-2021
Score :
Application Tested : WB
Sample Species : Hu
Figure Note : The data was peer reviewed and published in the journal PLoS One in 2015. PMID: 25928539
Date : Anonymous submitted on 18-Mar-2021
Score :
Application Tested : WB
Sample Species : Hu
Figure Note : The data was peer reviewed and published in the journal PLoS One in 2015. PMID: 25928539
Date : Anonymous submitted on 18-Mar-2021
Score :
Application Tested : WB
Sample Species : Hu
Figure Note : The data was peer reviewed and published in the journal PLoS One in 2015. PMID: 25876076
Date : Anonymous submitted on 18-Mar-2021
Score :
Application Tested : WB
Sample Species : Hu
Figure Note : The data was peer reviewed and published in the journal PLoS One in 2015. PMID: 25811469
Date : Anonymous submitted on 18-Mar-2021
Score :
Application Tested : WB
Sample Species : Hu
Figure Note : The data was peer reviewed and published in the journal PLoS One in 2015. PMID: 25811469
Date : Anonymous submitted on 18-Mar-2021
Score :
Application Tested : WB
Sample Species : Hu
Figure Note : The data was peer reviewed and published in the journal PLoS One in 2015. PMID: 25811469
Date : Anonymous submitted on 18-Mar-2021
Score :
Application Tested : WB
Sample Species : Hu
Figure Note : The data was peer reviewed and published in the journal PLoS One in 2015. PMID: 25738497
Date : Anonymous submitted on 18-Mar-2021
Score :
Application Tested : WB
Sample Species : Hu
Figure Note : The data was peer reviewed and published in the journal PLoS One in 2015. PMID: 25738497
Date : Anonymous submitted on 18-Mar-2021
Score :
Application Tested : WB
Sample Species : Hu
Figure Note : The data was peer reviewed and published in the journal Brain Behav in 2013. PMID: 24363972
Date : Anonymous submitted on 18-Mar-2021
Score :
Application Tested : WB
Sample Species : Hu
Figure Note : The data was peer reviewed and published in the journal J Cell Mol Med in 2020. PMID: 31821701
Date : Anonymous submitted on 18-Mar-2021
Score :
Application Tested : WB
Sample Species : Hu
Figure Note : The data was peer reviewed and published in the journal J Cell Mol Med in 2020. PMID: 31821701
Date : Anonymous submitted on 18-Mar-2021
Score :
Application Tested : WB
Sample Species : Hu
Figure Note : The data was peer reviewed and published in the journal Biomolecules in 2019. PMID: 31505885
Date : Anonymous submitted on 18-Mar-2021
Score :
Application Tested : WB
Sample Species : Hu
Figure Note : The data was peer reviewed and published in the journal Cell Adh Migr in 2019. PMID: 31240993
Date : Anonymous submitted on 18-Mar-2021
Score :
Application Tested : WB
Sample Species : Hu
Figure Note : The data was peer reviewed and published in the journal Acta Neuropathol in 2019. PMID: 30874922
Date : Anonymous submitted on 18-Mar-2021
Score :
Application Tested : WB
Sample Species : Hu
Figure Note : The data was peer reviewed and published in the journal PLoS Pathog in 2019. PMID: 30742689
Date : Anonymous submitted on 18-Mar-2021
Score :
Application Tested : WB
Sample Species : Hu
Figure Note : The data was peer reviewed and published in the journal PLoS Pathog in 2019. PMID: 30742689
Date : Anonymous submitted on 18-Mar-2021
Score :
Application Tested : WB
Sample Species : Hu
Figure Note : The data was peer reviewed and published in the journal PLoS Pathog in 2019. PMID: 30742689
Date : Anonymous submitted on 18-Mar-2021
Score :
Application Tested : WB
Sample Species : Hu
Figure Note : The data was peer reviewed and published in the journal Cancers (Basel) in 2019. PMID: 30634502
Date : Anonymous submitted on 18-Mar-2021
Score :
Application Tested : WB
Sample Species : Hu
Figure Note : The data was peer reviewed and published in the journal Oncotarget in 2018. PMID: 29899821
Date : Anonymous submitted on 18-Mar-2021
Score :
Application Tested : WB
Sample Species : Hu
Figure Note : The data was peer reviewed and published in the journal Oncotarget in 2016. PMID: 27191263
Date : Anonymous submitted on 18-Mar-2021
Score :
Application Tested : WB
Sample Species : Hu
Figure Note : The data was peer reviewed and published in the journal PLoS One in 2015. PMID: 25738497
Date : Anonymous submitted on 18-Mar-2021
Score :
Application Tested : WB
Sample Species : Ms
Figure Note : The data was peer reviewed and published in the journal Cell Death Differ in 2019. PMID: 30237510
Date : Anonymous submitted on 18-Mar-2021
Score :
Application Tested : WB
Sample Species : Hu
Figure Note : The data was peer reviewed and published in the journal Sci Rep in 2017. PMID: 28216671
Date : Anonymous submitted on 18-Mar-2021
Score :
Application Tested : WB
Sample Species : Hu
Figure Note : The data was peer reviewed and published in the journal PLoS Pathog in 2015. PMID: 25816318
Date : Anonymous submitted on 13-Aug-2018
Score :
Application Tested : WB
Sample Species : Hu
Sample : SAS cells
Amount used : 29 μg
Blocking : BSA, 25°C, 1Hr
Primary Antibody : 1:5000, 4°C, 16Hr
Date : Anonymous submitted on 31-Jul-2017
Score :
Application Tested : WB
Sample Species : Hu
Sample : A549 cells
Amount used : 30 μg
Blocking : 5% skim milk, 25°C, 1Hr
Primary Antibody : 1:5000, 25°C, 2Hr
Date : Anonymous submitted on 29-Apr-2016
Score :
Application Tested : WB
Sample Species : Ms
Sample : Brown adipose tissue
Amount used : 32μg
Blocking : 5% milk, 25°C, 1Hr
Primary Antibody : 1:1000, 4°C, 16Hr
Date : Anonymous submitted on 07-Apr-2015
Score :
Application Tested : WB
Sample Species : Ms
Sample : skin tissue
Amount used : 20μg
Blocking : 5% non-fat milk, 25°C, 2Hr
Primary Antibody : 1:10000, 4°C, 16Hr
Date : Anonymous submitted on 27-Jan-2015
Score :
Application Tested : WB
Sample Species : Rat
Sample : A7R5 cells, H9C2 cells
Amount used : 40μg
Blocking : 3% BSA in TBST, 25°C, 1Hr
Primary Antibody : 1:10000, 4°C, 24Hr
Lane Description : Lane1: A7R5 cells Lane2: H9C2 cells
Date : Anonymous submitted on 09-Oct-2014
Score :
Application Tested : WB
Sample Species : Hu
Sample : U2OS cells
Amount used : 2μg
Blocking : 5% milk in 0.1%TBST, 25°C, 0.5Hr
Primary Antibody : 1:20000, 25°C, 1Hr
Date : Anonymous submitted on 07-Aug-2014
Score :
Application Tested : WB
Sample Species : Hu
Sample : human lung cancer cells
Amount used : 30μg
Blocking : 25°C, 2Hr
Primary Antibody : 1:10000, 4°C, 10Hr
Date : Anonymous submitted on 09-May-2014
Score :
Application Tested : WB
Sample Species : Hu
Sample : 293T cells
Amount used : 28μg
Blocking : 5% BSA in TBST, 4°C, 1Hr
Primary Antibody : 1:5000, 4°C, 14Hr
Date : Anonymous submitted on 05-Jun-2013
Score :
Application Tested : WB
Sample Species : Hu
Sample : A549 cells,HCC827 cells,H1975 cells,H358 cells
Amount used : 50μg
Blocking : 5% milk, 25°C, 1Hr
Primary Antibody : 1:5000, 25°C, 1Hr
Date : Anonymous submitted on 16-Nov-2012
Score :
Application Tested : WB
Sample Species : Hu
Sample : HEK293T cells
Amount used : 30μg
Blocking : 1% BSA/TBST, 2Hr
Primary Antibody : 1:1000, 4°C, 16Hr
Date : Anonymous submitted on 24-Aug-2012
Score :
Application Tested : ICC/IF
Sample Species : Hu
Sample : HEK293 cells
Blocking : 1% BSA, 37°C, 0.5Hr
Fixation : 4% paraformaldehyde
Permeabilization /Antigen retrieval : 0.1% Triton X-100
Primary Antibody : 1:500, 37°C, 1Hr
Date : Anonymous submitted on 24-Aug-2012
Score :
Application Tested : WB
Sample : Drosophila eye disc tissue
Blocking : 5% milk in PBST, 25°C, 1Hr
Primary Antibody : 1:3000, 4°C, 16Hr
Date : Anonymous submitted on 30-Jan-2012
Score :
Application Tested : WB
Sample Species : Ms
Sample : Mouse cerebral endothelial tissue
Amount used : 30μg
Blocking : 5% skim milk in TBST, 4°C, overnightHr
Primary Antibody : 1:5000, 25°C, 2Hr
Date : Anonymous submitted on 16-Jan-2012
Score :
Application Tested : WB
Sample Species : Hu
Sample : SH-SY5Y cells
Amount used : 25μg
Blocking : 5% non-fat milk, 1Hr
Primary Antibody : 1:1000, 4°C, 16Hr
Date : Anonymous submitted on 16-Jan-2012
Score :
Application Tested : WB
Sample Species : Hu
Sample : A431 cells
Amount used : 30μg
Blocking : 5% BSA, 25°C, 1Hr
Primary Antibody : 1:60000, 4°C, 16Hr
Date : Anonymous submitted on 31-Oct-2011
Score :
Application Tested : WB
Sample Species : Hu
Sample : A549 cells
Amount used : 10μg
Blocking : 5% milk in TBST, 25°C, 2Hr
Primary Antibody : 1:1000, 25°C, 2Hr
Application Reference
Wang Y et al. BMC Mol Cell Biol 2022; 23 (1):49
Application : WB
Reactivity : Human
Tangeda V et al. Cell Death Dis 2022; 13 (3):241
Application : WB
Reactivity : Human
Kuo KL et al. Am J Cancer Res 2021; 11 (1):171-180
Application : WB
Reactivity : Human
Li M et al. Cancer Manag Res 2020; 12:9159-9171
Application : WB
Reactivity : Human
M?ltgen S et al. Cells 2020; 9 (6)
Application : WB
Reactivity : Human
Yang PM et al. Biomolecules 2020; 10 (4)
Application : WB
Reactivity : Human
Kuo KL et al. Cells 2019; 8 (10)
Application : WB
Reactivity : Human
Wei TT et al. Oncogene 2019; (Epub)
Application : WB
Reactivity : Human
Tsortouktzidis D et al. Metab Brain Dis 2019;
Application : WB
Reactivity : Human
Wangta Liu et al. Nucleic Acids 2019; 17:P477-490
Application : WB
Reactivity : Human
Kuo KL et al. Int J Mol Sci 2019; 20 (13)
Application : WB
Reactivity : Human
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