GAPDH antibody [N2C1], Internal
|Applications||ICC/IF, IHC-P, WB|
Not tested in other applications.
|IHC (Formalin-fixed paraffin-embedded sections)||1:100-1:1000*
*Optimal dilutions/concentrations should be determined by the researcher.
|Predicted Target Size||36 kDa (note)
|Positive Controls||A431 , H1299 , HeLa , HepG2 , Neuro2A , BCL-1 , Raw264.7 , *SH-SY5Y|
(Please refer to the vial label for the specific concentration)|
|Purification||Purified by antigen-affinity chromatography.|
|Full Name||glyceraldehyde-3-phosphate dehydrogenase|
|Product Description||Rabbit Polyclonal antibody to GAPDH (glyceraldehyde-3-phosphate dehydrogenase)|
|Background||The product of this gene catalyzes an important energy-yielding step in carbohydrate metabolism, the reversible oxidative phosphorylation of glyceraldehyde-3-phosphate in the presence of inorganic phosphate and nicotinamide adenine dinucleotide (NAD). The enzyme exists as a tetramer of identical chains. Many pseudogenes similar to this locus are present in the human genome. [provided by RefSeq]|
|Synonyms||G3PD antibody, GAPD antibody, MGC88685 antibody, GAPDH antibody, glyceraldehyde-3-phosphate dehydrogenase antibody, aging-associated gene 9 protein antibody, glyceraldehyde 3-phosphate dehydrogenase antibody, peptidyl-cysteine S-nitrosylase GAPDH antibody|
|Cellular Localization||Cytoplasm , perinuclear region , Membrane |
|Immunogen||Carrier-protein conjugated synthetic peptide encompassing a sequence within the center region of human GAPDH. The exact sequence is proprietary.|
|Species Reactivity||Human, Mouse|
|Predicted Cross Reactivity species Predicted cross-reactivity:|
Predicted cross-reactivity is based on sequence homology, with greater than 80% immunogen sequence identity considered positive.
Please note that we are only able to guarantee products to work in applications and species in which they have been tested.
|Rat, Dog, Cat, Pig, Rabbit, Rhesus Monkey|
|Predict Reactivity Note||Rat (100%), Dog (100%), Cat (100%), Pig (100%), Rabbit (100%), Rhesus Monkey (100%)|