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GATA1 antibody [4F5]

Anti-GATA1 antibody [4F5] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX82984
Anti-GATA1 antibody [4F5] used in IHC (Paraffin sections) (IHC-P). GTX82984
Anti-GATA1 antibody [4F5] used in Western Blot (WB). GTX82984

Cat. No. GTX82984

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 4F5

Isotype

 IgG2b

Application

 WB, ICC/IF, IHC-P, ELISA

Reactivity

 Human
Package
100 μl ($399)
See all GATA1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1/500 - 1/2000
ICC/IF 1/200 - 1/1000
IHC-P 1/200 - 1/1000
ELISA 1/10000
Not tested in other applications.

Calculated MW

43 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

Ascites

Preservative

0.03% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Antigen Species

Human

Immunogen

Purified recombinant fragment of human GATA1 expressed in E. Coli.

Purification

Unpurified

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

GATA binding protein 1 , ERYF1 , GATA-1 , GF-1 , GF1 , NF-E1 , NFE1 , XLANP , XLTDA , XLTT

Cellular Localization

Nucleus

Background

This gene encodes a protein which belongs to the GATA family of transcription factors. The protein plays an important role in erythroid development by regulating the switch of fetal hemoglobin to adult hemoglobin. Mutations in this gene have been associated with X-linked dyserythropoietic anemia and thrombocytopenia. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-GATA1 antibody [4F5] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX82984

GTX82984 ICC/IF Image

ICC/IF analysis of K562(left) cells using GTX82984 GATA1 antibody [4F5].
Green : GATA1
Blue: DRAQ5 fluorescent DNA dye

Anti-GATA1 antibody [4F5] used in IHC (Paraffin sections) (IHC-P). GTX82984

GTX82984 IHC-P Image

IHC-P analysis of pancreatic cancer using GTX82984 GATA1 antibody [4F5].

Anti-GATA1 antibody [4F5] used in Western Blot (WB). GTX82984

GTX82984 WB Image

WB analysis of K562 cell lysate using GTX82984 GATA1 antibody [4F5].

REFERENCE

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REVIEW

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SDS
Sodium Azide.pdf
Package List Price ($)
$ 399