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GATA4 antibody

Anti-GATA4 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX113194
Anti-GATA4 antibody used in IHC (Paraffin sections) (IHC-P). GTX113194
Anti-GATA4 antibody used in IHC (Paraffin sections) (IHC-P). GTX113194
Anti-GATA4 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX113194
Anti-GATA4 antibody used in Western Blot (WB). GTX113194
Anti-GATA4 antibody used in IHC (Paraffin sections) (IHC-P). GTX113194
Anti-GATA4 antibody used in IHC (Paraffin sections) (IHC-P). GTX113194
Anti-GATA4 antibody used in Western Blot (WB). GTX113194
Anti-GATA4 antibody used in IHC (Paraffin sections) (IHC-P). GTX113194

Cat. No. GTX113194

Reference 6
1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Mouse
Package
100 μl ($399),
25 μl ($169)
See all GATA4 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

45 kDa. ( Note )

Predict Reactivity

Rat, Bovine, Dog, Chicken, Pig, Xenopus tropicalis(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.21 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human GATA4. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_10726138

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

GATA binding protein 4 , ASD2 , TACHD , TOF , VSD1

Cellular Localization

Nucleus

Background

This gene encodes a member of the GATA family of zinc-finger transcription factors. Members of this family recognize the GATA motif which is present in the promoters of many genes. This protein is thought to regulate genes involved in embryogenesis and in myocardial differentiation and function. Mutations in this gene have been associated with cardiac septal defects. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-GATA4 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX113194

GTX113194 ICC/IF Image

GATA4 antibody detects GATA4 protein at nucleus by immunofluorescent analysis.
Sample: HepG2 cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: GATA4 stained by GATA4 antibody (GTX113194) diluted at 1:500.
Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:1000.

Anti-GATA4 antibody used in IHC (Paraffin sections) (IHC-P). GTX113194

GTX113194 IHC-P Image

GATA4 antibody detects GATA4 protein at nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded mouse E13.5 embryo.
Green: GATA4 stained by GATA4 antibody (GTX113194) diluted at 1:250.
Blue: Fluoroshield with DAPI (GTX30920).
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-GATA4 antibody used in IHC (Paraffin sections) (IHC-P). GTX113194

GTX113194 IHC-P Image

GATA4 antibody detects GATA4 protein at nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded mouse colon.
GATA4 stained by GATA4 antibody (GTX113194) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-GATA4 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX113194

GTX113194 ICC/IF Image

Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using GATA4(GTX113194) antibody at 1:500 dilution.

Anti-GATA4 antibody used in Western Blot (WB). GTX113194

GTX113194 WB Image

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with GATA4 antibody (GTX113194) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-GATA4 antibody used in IHC (Paraffin sections) (IHC-P). GTX113194

GTX113194 IHC-P Image

GATA4 antibody detects GATA4 protein at nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded mouse stomach.
GATA4 stained by GATA4 antibody (GTX113194) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-GATA4 antibody used in IHC (Paraffin sections) (IHC-P). GTX113194

GTX113194 IHC-P Image

GATA4 antibody detects GATA4 protein at nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded mouse duodenum.
GATA4 stained by GATA4 antibody (GTX113194) diluted at 1:1000.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-GATA4 antibody used in Western Blot (WB). GTX113194

GTX113194 WB Image

The data was published in the journal Sci Rep in 2017. PMID: 28102204

Anti-GATA4 antibody used in IHC (Paraffin sections) (IHC-P). GTX113194

GTX113194 IHC-P Image

The data was published in the journal Sci Rep in 2017. PMID: 28102204

REFERENCE

Application Reference

CH Wang et al. Regen Ther 2023; 24 : 443-450 Cbx7 promotes the generation of induced pluripotent stem cells
Application : ICC/IF
Lee YH et al. Theranostics 2019; 9 (22) : 6443-6465 Role of FOXC1 in regulating APSCs self-renewal via STI-1/PrPC signaling.
Application : ICC/IF
Reactivity : Human
Abdelmonem M et al. Biomed Pharmacother 2019; 112 : 108584 Hydrogen sulfide enhances the effectiveness of mesenchymal stem cell therapy in rats with heart failure: In vitro preconditioning versus in vivo co-delivery.
Lao S et al. Mol Med Rep 2017; 16 (1) : 507-514 A comparative study of the influence of two types of PHEMA stents on the differentiation of ASCs to myocardial cells.
Application : WB
Reactivity : Human
Qian Y et al. J Transl Med 2017; 15 (1) : 69 Multiple gene variations contributed to congenital heart disease via GATA family transcriptional regulation.
Application : WB
Reactivity : Human
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REVIEW

GATA4 antibody Cat. No. GTX113194

Rating
( Average 5 based on 1 users reviews)
Application
Western Blot(WB)
( Average 5 based on 1 users reviews)
Anti-GATA4 antibody used in Western Blot (WB). GTX113194
Date : Anonymous submitted on 15-Apr-2014
Score :
Application Tested : WB
Sample Species : Ms
Sample : mouse heart tissue
Amount used : 8μg
Blocking : 3% milk in PBST, 23°C, 1Hr
Primary Antibody : 1:500, 4°C, 20Hr
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SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 399
$ 169