Glucokinase antibody [3E3]

Anti-Glucokinase antibody [3E3] used in Western Blot (WB). GTX84440

Cat. No. GTX84440

Host	Mouse
Clonality	Monoclonal
Clone Name	3E3
Isotype	IgG1
Application	WB, ICC/IF, IHC-P
Reactivity	Human
Package	100 μl ($519)

See all Glucokinase products

Datasheet
Document

View product citations for antibody GTX84440 on CiteAb

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Recommended Dilution
WB	1:1000-1:2000
ICC/IF	1:50
IHC-P	1:50

Not tested in other applications.

Calculated MW

52 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Full length human recombinant protein of human GCK (NP_000153) produced in HEK293T cell.

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

glucokinase , FGQTL3 , GK , GLK , HHF3 , HK4 , HKIV , HXKP , LGLK , MODY2

Cellular Localization

Cytoplasm,Nucleus

Background

Catalyzes the initial step in utilization of glucose by the beta-cell and liver at physiological glucose concentration. Glucokinase has a high Km for glucose, and so it is effective only when glucose is abundant. The role of GCK is to provide G6P for the synthesis of glycogen. Pancreatic glucokinase plays an important role in modulating insulin secretion. Hepatic glucokinase helps to facilitate the uptake and conversion of glucose by acting as an insulin-sensitive determinant of hepatic glucose usage.

Database

Gene ID: 2645 GCK
UniProt: P35557 GCK

Research Area

DATA IMAGES

GTX84440 IHC-P Image

IHC-P analysis of human lung carcinoma tissue using GTX84440 Glucokinase antibody [3E3].
Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100ºC for 10min.

GTX84440 IHC-P Image

IHC-P analysis of human prostate carcinoma tissue using GTX84440 Glucokinase antibody [3E3].
Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100ºC for 10min.

GTX84440 ICC/IF Image

ICC/IF analysis of COS7 cells transiently transfected with Glucokinase plasmid using GTX84440 Glucokinase antibody [3E3].

GTX84440 IHC-P Image

IHC-P analysis of human pancreas tissue using GTX84440 Glucokinase antibody [3E3].
Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100ºC for 10min.

GTX84440 IHC-P Image

IHC-P analysis of human kidney tissue using GTX84440 Glucokinase antibody [3E3].
Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100ºC for 10min.

GTX84440 IHC-P Image

IHC-P analysis of human thyroid carcinoma tissue using GTX84440 Glucokinase antibody [3E3].
Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100ºC for 10min.

GTX84440 IHC-P Image

IHC-P analysis of human breast tissue using GTX84440 Glucokinase antibody [3E3].
Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100ºC for 10min.

GTX84440 IHC-P Image

IHC-P analysis of human lung tissue using GTX84440 Glucokinase antibody [3E3].
Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100ºC for 10min.

GTX84440 IHC-P Image

IHC-P analysis of human kidney carcinoma tissue using GTX84440 Glucokinase antibody [3E3].
Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100ºC for 10min.

GTX84440 IHC-P Image

IHC-P analysis of human breast adenocarcinoma tissue using GTX84440 Glucokinase antibody [3E3].
Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100ºC for 10min.

GTX84440 WB Image

WB analysis of HEK293T cells transfected with Glucokinase plasmid (Right) or empty vector (Left) for 48 hrs using GTX84440 Glucokinase antibody [3E3].
Loading : 5 ug per lane

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf

Package

List Price ($)

100 μl

$ 519

Qty

Glucokinase antibody [3E3]

Cat. No. GTX84440

Host

Clonality

Clone Name

Isotype

Application

Reactivity

APPLICATION

Application Note

Calculated MW

PROPERTIES

Form

Buffer

Preservative

Storage

Concentration

Antigen Species

Immunogen

Purification

Conjugation

Note

TARGET

Synonyms

Cellular Localization

Background

Database

Research Area

DATA IMAGES

GTX84440 IHC-P Image

GTX84440 IHC-P Image

GTX84440 ICC/IF Image

GTX84440 IHC-P Image

GTX84440 IHC-P Image

GTX84440 IHC-P Image

GTX84440 IHC-P Image

GTX84440 IHC-P Image

GTX84440 IHC-P Image

GTX84440 IHC-P Image

GTX84440 WB Image

REFERENCE

REVIEW

Product

Research

Support

Company

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