|Applications||ICC/IF, IHC-P, IP, WB|
Not tested in other applications.
|IHC (Formalin-fixed paraffin-embedded sections)||1:100-1:1000*
*Optimal dilutions/concentrations should be determined by the researcher.
|Predicted Target Size||112 kDa (note)
|Positive Controls||293T , A431 , BCL-1 , C2C12 , C8D30 , GL261 , HeLa , HepG2 , NIH-3T3 , Neuro 2A , Raw264.7 , hMSC-3A6 , *mouse fibroblast|
(Please refer to the vial label for the specific concentration)|
|Purification||Purified by antigen-affinity chromatography.|
|Full Name||Rho/Rac guanine nucleotide exchange factor (GEF) 2|
|Product Description||Rabbit Polyclonal antibody to GEF-H1 (Rho/Rac guanine nucleotide exchange factor (GEF) 2)|
|Background||Rho GTPases play a fundamental role in numerous cellular processes that are initiated by extracellular stimuli that work through G protein coupled receptors. The encoded protein may form complex with G proteins and stimulate rho-dependent signals. [provided by RefSeq]|
|Synonyms||DKFZp547L106 antibody, DKFZp547P1516 antibody, FLJ45440 antibody, GEF antibody, GEF-H1 antibody, GEFH1 antibody, KIAA0651 antibody, LFP40 antibody, P40 antibody, ARHGEF2 antibody, microtubule-regulated Rho-GEF antibody, Rho/Rac guanine nucleotide exchange factor 2 antibody, proliferating cell nucleolar antigen p40 antibody, rho guanine nucleotide exchange factor 2 antibody, guanine nucleotide exchange factor H1 antibody, Rho/Rac guanine nucleotide exchange factor (GEF) 2 antibody|
|Cellular Localization||Cytoplasm , Cell junction , tight junction , Golgi apparatus , Spindle |
|Immunogen||Recombinant protein encompassing a sequence within the C-terminus region of human GEF-H1. The exact sequence is proprietary.|
|Species Reactivity||Human, Mouse, Rat|
|Predicted Cross Reactivity species Predicted cross-reactivity:|
Predicted cross-reactivity is based on sequence homology, with greater than 80% immunogen sequence identity considered positive.
Please note that we are only able to guarantee products to work in applications and species in which they have been tested.
|Dog, Pig, Bovine|
|Predict Reactivity Note||Dog (92%), Pig (96%), Bovine (94%)|