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1 .GTX113617 GFP antibody

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ECL kit RIPA Extraction Kit Protein Ladder

GFP antibody


  • Host / ClonalityRabbit Polyclonal
  • ApplicationsELISA, ICC/IF, IHC, IHC-Fr, IHC-P, IP, WB, IHC-Wm
  • ★★★★★   (  4  ) Publication  ( 59 )
Cat No: GTX113617
Package Price Qty
25 μlUSD 119.00
100 μlUSD 319.00
Add to cart
 
  • Anti-GFP antibody used in IHC (Whole mount). GTX113617
  • Anti-GFP antibody used in Western Blot (WB). GTX113617
  • Anti-GFP antibody used in Western Blot (WB). GTX113617
  • Anti-GFP antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX113617
  • Anti-GFP antibody used in Immunoprecipitation. GTX113617
  • Anti-GFP antibody used in Western Blot (WB). GTX113617
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  • Datasheet
  • Star Review (4)
    Reference (59)
  • Protocol & MSDS
  • Application Information: GFP antibody

Catalog Number GTX113617
★★★★★
( 4 )
Publication ( 59 )
Product Name GFP antibody
See all GFP products
ApplicationsELISA, ICC/IF, IHC, IHC-Fr, IHC-P, IP, WB, IHC-Wm
Application Note
Suggested dilutionReference
ELISAAssay-dependent dilution Y
Immunocytochemistry/ Immunofluorescence (ICC/IF)1:1000-1:5000* Y
Immunohistochemistry (IHC)Assay-dependent dilution Y
IHC (Frozen sections)Assay-dependent dilution Y
IHC (Formalin-fixed paraffin-embedded sections)Assay-dependent dilution Y
Immunoprecipitation1:100-1:500* Y
Western Blot (WB)1:5000-1:20000* Y
IHC (Whole mount)1:100-1:500* Y
Not tested in other applications.
*Optimal dilutions/concentrations should be determined by the researcher.
Predicted Target Size29 kDa (note)
Positive Controlshuman GFP-transfected 293T , 293T whole cell extract expressing GFP-tagged protein , *GFP-p53 transfected U2OS
Form SuppliedLiquid
Concentration1 mg/ml (Please refer to the vial label for the specific concentration)
PurificationPurified by antigen-affinity chromatography.

  • Specifications: GFP antibody

Full NameGreen Fluorescent Protein
SynonymsGreen Fluorescent Protein, GFP, eGFP, enhanced green fluorescent protein
SpecificityThis antibody reacts with GFP variants including GFP, EGFP, and YFP.
HostRabbit
ClonalityPolyclonal
IsotypeIgG
ImmunogenFull length GFP recombinant protein
ConjugationUnconjugated

  • Storage Conditions: GFP antibody

Storage Buffer1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.
Storage InstructionKeep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
NotesFor In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
ResearchArea
  • Epitope Tag > GFP
More Hide
GFP antibody validated data
  • Anti-GFP antibody used in IHC (Whole mount). GTX113617
    • GTX113617 IHC-Wm Image
      Immunohistochemical analysis (whole mount) of transgenic zebrafish embryo, using GFP(GTX113617) antibody at 1:200 dilution.
      Top
  • Anti-GFP antibody used in Western Blot (WB). GTX113617
    • GTX113617 WB Image
      IP-WB assay to show that Hice1 (GTX113617) co-immunoprecipitated with other Augmin components HAUS6 (GTX118732), HAUS2 (GTX118734) and HAUS1 (GTX118733) in U2OS cells. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
      Top
  • Anti-GFP antibody used in Western Blot (WB). GTX113617
    • GTX113617 WB Image
      GFP antibody detects GFP protein by western blot analysis.
      A. 1 µg 293T whole cell extract
      B. 1 µg 293T whole cell extract expressing GFP-tagged protein
      C. 1 µg 293T whole cell extract expressing RFP-tagged protein
      12% SDS-PAGE
      GFP antibody (GTX113617) dilution: 1:10000
      The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
      Top
  • Anti-GFP antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX113617
    • GTX113617 ICC/IF Image
      Immunofluorescence analysis of GFP-transfected HeLa. A:GFP is expressed in the tranfected cell. B: The cell expressing GFP can be detected using rabbit anti-GFP ab ( GTX113617, 1:5000) followed by Alexa Fluor 594 (1:500) goat anti-rabbit IgG. C: Merged with DNA probe, the lower cell with no GFP expressed as a negtive control.
      Top
  • Anti-GFP antibody used in Immunoprecipitation. GTX113617
    • GTX113617 IP Image
      GFP was immunoprecipitated from bacterial GFP-expressing lysate using 1 ug of anti-GFP antibody (GTX113617).
      The precipitated GFP was detected by GTX113617 diluted at 1:15000.
      Top
  • Anti-GFP antibody used in Western Blot (WB). GTX113617
    • GTX113617 WB Image
      GFP antibody detects GFP protein by western blot analysis. Non-transfected (-) and GFP-transfected (+, ) 293T whole cell extracts (1 µg) were separated by 12% SDS-PAGE, and the membrane was blotted with GFP antibody (GTX113617) at a dilution of 1:10000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
      Top
  • Anti-GFP antibody used in IHC (Whole mount). GTX113617
    • GTX113617 IHC-Wm Image
      Immunohistochemical analysis (whole mount) of transgenic zebrafish embryo, using GFP(GTX113617) antibody at 1:200 dilution.
      Top
  • Anti-GFP antibody used in Western Blot (WB). GTX113617
    • GTX113617 WB Image
      IP-WB assay to show that Hice1 (GTX113617) co-immunoprecipitated with other Augmin components HAUS6 (GTX118732), HAUS2 (GTX118734) and HAUS1 (GTX118733) in U2OS cells. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
      Top
  • Anti-GFP antibody used in Western Blot (WB). GTX113617
    • GTX113617 WB Image
      GFP antibody detects GFP protein by western blot analysis.
      A. 1 µg 293T whole cell extract
      B. 1 µg 293T whole cell extract expressing GFP-tagged protein
      C. 1 µg 293T whole cell extract expressing RFP-tagged protein
      12% SDS-PAGE
      GFP antibody (GTX113617) dilution: 1:10000
      The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
      Top
  • Anti-GFP antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX113617
    • GTX113617 ICC/IF Image
      Immunofluorescence analysis of GFP-transfected HeLa. A:GFP is expressed in the tranfected cell. B: The cell expressing GFP can be detected using rabbit anti-GFP ab ( GTX113617, 1:5000) followed by Alexa Fluor 594 (1:500) goat anti-rabbit IgG. C: Merged with DNA probe, the lower cell with no GFP expressed as a negtive control.
      Top
  • Anti-GFP antibody used in Immunoprecipitation. GTX113617
    • GTX113617 IP Image
      GFP was immunoprecipitated from bacterial GFP-expressing lysate using 1 ug of anti-GFP antibody (GTX113617).
      The precipitated GFP was detected by GTX113617 diluted at 1:15000.
      Top
  • Anti-GFP antibody used in Western Blot (WB). GTX113617
    • GTX113617 WB Image
      GFP antibody detects GFP protein by western blot analysis. Non-transfected (-) and GFP-transfected (+, ) 293T whole cell extracts (1 µg) were separated by 12% SDS-PAGE, and the membrane was blotted with GFP antibody (GTX113617) at a dilution of 1:10000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
      Top
  • StarReview
  • GFP antibody

  • Rating : ★★★★★ ( Average 5.0 based on 4 users reviews)
  • Immunohistochemistry (IHC)(1) :★★★★★
  • Western Blot (WB)(3) :★★★★☆
  • Anonymous StarReview submitted on 22 Feb 2018
  • Score : ★★★★★
  • Application Tested : IHC
  • Sample : Drosophila Retinal tissue
  • Blocking conditions
  • Blocking Buffer : 10% NGS in 0.3% PBST (triton-X-100)
  • Incubation Time & Temperature : 25 (℃)/1 hr
  • Primary antibody
  • Dilution : 1:200( conc. of dilution )
  • Incubation Time & Temperature : 4 (℃)/overnight
  • Data Description
  • Green: GTX113617 GFP antibody: photoreceptor cell R8, Red: GTX630189 mcherry antibody: medulla
  • Anonymous StarReview submitted on 12 Apr 2012
  • Score : ★★★★★
  • Application Tested : WB
  • Sample Species : Arobidopsis thiliana
  • Sample : Protoplast from Rosette leaves
  • Amount used(ug/cells) : 30ug
  • Blocking conditions
  • Blocking Buffer : 5% milk in PBST
  • Incubation Time & Temperature : 4℃ (℃)/16hr
  • Primary antibody
  • Dilution : 1:10000( conc. of dilution )
  • Incubation Time & Temperature : RT (℃)/1hr
  • Anonymous StarReview submitted on 09 Oct 2014
  • Score : ★★★★☆
  • Application Tested : WB
  • Sample : GFP-p53 transfected U2OS cells
  • Amount used(ug/cells) : 15ug
  • Blocking conditions
  • Blocking Buffer : 5% milk in 0.1%TBST
  • Incubation Time & Temperature : 25 (℃)/0.5hr
  • Primary antibody
  • Dilution : 1:5000( conc. of dilution )
  • Incubation Time & Temperature : 25 (℃)/1hr
  • Data Description
  • A: U2OS cells transfected with GFP-p53 B: U2OS cells transfected with control plasmid (no GFP-tag)
  • Anonymous StarReview submitted on 09 Oct 2014
  • Score : ★★★★★
  • Application Tested : WB
  • Sample : GFP recombinant protein transfected 293T cells
  • Amount used(ug/cells) : 20ug
  • Blocking conditions
  • Blocking Buffer : 5% milk
  • Incubation Time & Temperature : RT (℃)/1hr
  • Primary antibody
  • Dilution : 1:5000( conc. of dilution )
  • Incubation Time & Temperature : 4 (℃)/overnight
Back to Top
  • Reference for GFP antibody
Application References Application Reactivity
Dodson M et al. Increased O-GlcNAcylation of SNAP29 drives arsenic-induced autophagic dysfunction. Mol Cell Biol (Epub ahead of print) (2018).
Borga C et al. Simultaneous B and T cell acute lymphoblastic leukemias in zebrafish driven by transgenic MYC: implications for oncogenesis and lymphopoiesis. bioRxiv (Epub ahead of print) (2018). IHC-P
Zhu Y et al. Autophagy inhibition promotes phagocytosis of macrophage and protects mice from methicillin-resistant staphylococcus aureus pneumonia. J Cell Biochem (Epub ahead of print) (2018). ICC/IF
Chou YT et al. Identification of danthron as an isoform-specific inhibitor of HEME OXYGENASE-1/cytochrome P450 reductase interaction with anti-tumor activity. J Biomed Sci 25 (1):6 (2018). WB
Gao Z et al. PDGFRα / PDGFRβ signaling balance modulates progenitor cell differentiation into white and beige adipocytes. Development (Epub ahead of print) (2017).
van Ijzendoorn DGP et al. Functional Analyses of a Human Vascular Tumor FOS Variant Identify a Novel Degradation Mechanism and a link to Tumorigenesis. J Biol Chem (Epub ahead of print) (2017).
Tzeng YW et al. LMO7 exerts an effect on mitosis progression and the spindle assembly checkpoint. Int J Biochem Cell Biol 94:22-30 (2017). IP, WB
Suboti? A et al. A Bimolecular Fluorescence Complementation Tool for Identification of Protein-Protein Interactions in Candida albicans. G3 (Bethesda) 7 (10):3509-3520 (2017). WB
Chang CT et al. Inhibition of ROS production, autophagy or apoptosis signaling reversed the anticancer properties of Antrodia salmonea in triple-negative breast cancer (MDA-MB-231) cells. Food Chem Toxicol 103:1-17 (2017). ICC/IF
Solis GP et al. Golgi-Resident Gαo Promotes Protrusive Membrane Dynamics. Cell (Epub ahead of print) (2017). WB
Wu SC et al. Intestinal microbial dysbiosis aggravates the progression of Alzheimer's disease in Drosophila. Nat Commun 8 (1):24 (2017).
Tillotson J et al. Arsenic Compromises Both p97 and Proteasome Functions. Chem Res Toxicol 30 (7):1508-1514 (2017). WB
Abiria SA et al. TRPM7 senses oxidative stress to release Zn2+ from unique intracellular vesicles. Proc Natl Acad Sci U S A (2017). IP
Chang CT et al. Chalcone flavokawain B induces autophagic-cell death via reactive oxygen species-mediated signaling pathways in human gastric carcinoma and suppresses tumor growth in nude mice. Arch Toxicol (Epub ahead of print) (2017). WB
Harder B et al. Brusatol overcomes chemoresistance through inhibition of protein translation. Mol Carcinog (Epub ahead of print) (2016).
Xu Q et al. Analysis of the erythropoietin of a Tibetan Plateau schizothoracine fish (Gymnocypris dobula) reveals enhanced cytoprotection function in hypoxic environments. BMC Evol Biol 16:11 (2016). WB
Wong MT et al. Human Choline Kinase-α Promotes Hepatitis C Virus RNA Replication through Modulating Membranous Viral Replication Complex Formation. J Virol (2016). WB
Nyman JS et al. Combined treatment with a transforming growth factor beta inhibitor (1D11) and bortezomib improves bone architecture in a mouse model of myeloma-induced bone disease. Bone 91:81-91 (2016). IHC-P
Koizumi H et al. DCLK1 phosphorylates the microtubule-associated protein MAP7D1 to promote axon elongation in cortical neurons. Dev Neurobiol (2016).
Lai CW et al. Using Dual Fluorescence Reporting Genes to Establish an In Vivo Imaging Model of Orthotopic Lung Adenocarcinoma in Mice. Mol Imaging Biol (Epub ahead of print) (2016). WB, IHC-P
Lai CW et al. Sexually Dimorphic Expression of eGFP Transgene in the Akr1A1 Locus of Mouse Liver Regulated by Sex Hormone-Related Epigenetic Remodeling. Sci Rep 6:24023 (2016). WB, IHC-P
Xu Q et al. Analysis of the erythropoietin of a Tibetan Plateau schizothoracine fish (Gymnocypris dobula) reveals enhanced cytoprotection function in hypoxic environments. BMC Evol Biol 16 (1):11 (2016).
Ross E et al. AMP-Activated Protein Kinase Regulates the Cell Surface Proteome and Integrin Membrane Traffic. PLoS One 10 (5):e0128013 (2015). WB
Steinke KV et al. Human CLC-K Channels Require Palmitoylation of Their Accessory Subunit Barttin to Be Functional. J Biol Chem 290 (28):17390-400 (2015). IP
Yu H et al. Autonomous and non-autonomous roles of DNase?II during cell death in C. elegans embryos. Biosci Rep 35 (3) (2015). IHC-Wm
Yang FM et al. A calreticulin-dependent nuclear export signal is involved in the regulation of liver receptor homologue-1 protein folding. Biochem J 471 (2):199-209 (2015). IP
Dickmeis C et al. Production of Hybrid Chimeric PVX Particles Using a Combination of TMV and PVX-Based Expression Vectors. Front Bioeng Biotechnol 3:189 (2015). WB
Chen PJ et al. NPGPx modulates CPEB2-controlled HIF-1α RNA translation in response to oxidative stress. Nucleic Acids Res 43 (19):9393-404 (2015).
Li Q et al. Akt Phosphorylates Wnt Coactivator and Chromatin Effector Pygo2 at Serine 48 to Antagonize Its Ubiquitin/Proteasome-mediated Degradation. J Biol Chem 290 (35):21553-67 (2015).
Wu JM et al. Circulating cells contribute to cardiomyocyte regeneration after injury. Circ Res 116 (4):633-41 (2015). IHC-P
Lee WY et al. Repositioning antipsychotic chlorpromazine for treating colorectal cancer by inhibiting sirtuin 1. Oncotarget (Epub Ahead of Print) (2015). WB
Wu HC et al. Production and characterization of active recombinant interleukin-12/eGFP fusion protein in stably-transfected DF1 chicken cells. Biotechnol Prog (2015). ELISA
Chen CJ et al. SH2B1 and IRSp53 Proteins Promote the Formation of Dendrites and Dendritic Branches. J Biol Chem 290 (10):6010-21 (2015). ICC/IF, WB
Auer TO et al. Highly efficient CRISPR/Cas9-mediated knock-in in zebrafish by homology-independent DNA repair. Genome Res 24 (1):142-53 (2014). IHC-Wm
Hsueh YC et al. Prostaglandin E? promotes post-infarction cardiomyocyte replenishment by endogenous stem cells. EMBO Mol Med 6 (4):496-503 (2014). IHC-P
Willemen HL et al. Monocytes/Macrophages control resolution of transient inflammatory pain. J Pain 15 (5):496-506 (2014). IHC-Fr
Hong JB et al. A nucleolus-predominant piggyBac transposase, NP-mPB, mediates elevated transposition efficiency in mammalian cells. PLoS One 9 (2):e89396 (2014). WB
Pope-Varsalona H et al. Polymerase η suppresses telomere defects induced by DNA damaging agents. Nucleic Acids Res 42 (21):13096-109 (2014). ICC/IF
Huang IH et al. GEF-H1 controls focal adhesion signaling that regulates mesenchymal stem cell lineage commitment. J Cell Sci. 127 (Pt 19):4186-200 (2014). WB
Yu Z et al. Using a yeast two-hybrid system to identify FTCD as a new regulator for HIF-1α in HepG2 cells. Cell Signal 26 (7):1560-1566 (2014). WB
S Shukla et al. Molecular farming of fluorescent virus-based nanoparticles for optical imaging in plants, human cells and mouse models Biomater Sci (2014).
Hsu CY et al. The Epstein-Barr Virus-Encoded MicroRNA MiR-BART9 Promotes Tumor Metastasis by Targeting E-Cadherin in Nasopharyngeal Carcinoma. PLoS Pathog 10 (2):e1003974 (2014). IHC
Chang YJ et al. SH2B1β Interacts with STAT3 and Enhances Fibroblast Growth Factor 1-Induced Gene Expression during Neuronal Differentiation. Mol Cell Biol 34 (6):1003-19 (2014). IP, WB
Reetz J et al. Development of Adenoviral Delivery Systems to Target Hepatic Stellate Cells In Vivo. PLoS One 8 (6):e67091 (2013). IHC-P
Molleken K et al. The Chlamydia pneumoniae invasin protein Pmp21 recruits the EGF receptor for host cell entry. PLoS Pathog 9 (4):e1003325 (2013).
Wirth A et al. Dual lipidation of the brain-specific Cdc42 isoform regulates its functional properties. Biochem J 456 (3):311-22 (2013).
Barak LS et al. Triphenylmethane dye activation of beta-arrestin. Biochemistry 52 (32):5403-14 (2013).
Chang RY et al. Japanese encephalitis virus non-coding RNA inhibits activation of interferon by blocking nuclear translocation of interferon regulatory factor 3. Vet Microbiol 166 (1-2):11-21 (2013). IP
Park J et al. RAS-MAPK-MSK1 pathway modulates ataxin 1 protein levels and toxicity in SCA1. Nature 498 (7454):325-31 (2013). WB
Wang H et al. Balancing GRK2 and EPAC1 levels prevents and relieves chronic pain. J Clin Invest 123 (12):5023-34 (2013). IHC-Fr
He G et al. Identification of liver cancer progenitors whose malignant progression depends on autocrine IL-6 signaling. Cell 155 (2):384-96 (2013). IHC-P
Lin LT et al. mPlum-IFP 1.4 fluorescent fusion protein may display Förster resonance energy transfer associated properties that can be used for near-infrared based reporter gene imaging. J Biomed Opt 18 (12):126013 (2013). WB
Hsu WB et al. Cellular protein HAX1 interacts with the influenza A virus PA polymerase subunit and impedes its nuclear translocation. J Virol 87 (1):110-23 (2013). WB
Chiang YJ et al. CBAP functions as a novel component in chemokine-induced ZAP70-mediated T-cell adhesion and migration. PLoS One 8 (4):e61761 (2013). WB
Tsou TC et al. Crucial role of Toll-like receptors in the zinc/nickel-induced inflammatory response in vascular endothelial cells. Toxicol Appl Pharmacol 273 (3):492-9 (2013). WB
Wei PC et al. Loss of the oxidative stress sensor NPGPx compromises GRP78 chaperone activity and induces systemic disease. Mol Cell 48 (5):747-59 (2012). WB
Lai SL et al. Autotaxin/Lpar3 signaling regulates Kupffer's vesicle formation and left-right asymmetry in zebrafish. Development 139 (23):4439-48 (2012).
Hsiao WC et al. Vitamin D3-inducible mesenchymal stem cell-based delivery of conditionally replicating adenoviruses effectively targets renal cell carcinoma and inhibits tumor growth. Mol Pharm 9 (5):1396-408 (2012). IHC
Kwanchanok S et al. In vitro fluorescent analysis of preprotein import into chloroplasts. Biosci Biotechnol Biochem 75 (10):2001-7 (2011). WB
    *MSDS
    MSDS-Glycerol.pdf
    MSDS-PBS.pdf
    MSDS-ProClin.pdf
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All products are for research use only—Not for use in diagnostic procedures.