GFP antibody

Host / ClonalityRabbit Polyclonal
ApplicationsELISA, ICC/IF, IHC, IHC-Fr, IHC-P, IP, WB, IHC-Wm
★★★★★ ( 4 ) ( 60 )

Cat No:

GTX113617

Package	Price	Qty
25 μl	USD 119.00
100 μl	USD 319.00

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Star Review (4)
Reference (60)
Protocol & MSDS

Application Information: GFP antibody

Catalog Number

GTX113617

Product Name

GFP antibody

See all GFP products

Applications

ELISA, ICC/IF, IHC, IHC-Fr, IHC-P, IP, WB, IHC-Wm

Application Note

	Suggested dilution	Reference
ELISA	Assay-dependent dilution	Y
Immunocytochemistry/ Immunofluorescence (ICC/IF)	1:1000-1:5000*	Y
Immunohistochemistry (IHC)	Assay-dependent dilution	Y
IHC (Frozen sections)	Assay-dependent dilution	Y
IHC (Formalin-fixed paraffin-embedded sections)	Assay-dependent dilution	Y
Immunoprecipitation	1:100-1:500*	Y
Western Blot (WB)	1:5000-1:20000*	Y
IHC (Whole mount)	1:100-1:500*	Y

Not tested in other applications.
*Optimal dilutions/concentrations should be determined by the researcher.

Predicted Target Size

29 kDa (note)

Positive Controls

human GFP-transfected 293T , 293T whole cell extract expressing GFP-tagged protein , *GFP-p53 transfected U2OS

Form Supplied

Liquid

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration)

Purification

Purified by antigen-affinity chromatography.

Specifications: GFP antibody

Full Name	Green Fluorescent Protein
Synonyms	Green Fluorescent Protein, GFP, eGFP, enhanced green fluorescent protein
Specificity	This antibody reacts with GFP variants including GFP, EGFP, and YFP.
Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Immunogen	Full length GFP recombinant protein
Conjugation	Unconjugated

Storage Conditions: GFP antibody

Storage Buffer	1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.
Storage Instruction	Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Notes	For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
ResearchArea
Epitope Tag > GFP More Hide

GFP antibody validated data

GTX113617 IHC-Wm Image

Immunohistochemical analysis (whole mount) of transgenic zebrafish embryo, using GFP(GTX113617) antibody at 1:200 dilution.

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GTX113617 WB Image

IP-WB assay to show that Hice1 (GTX113617) co-immunoprecipitated with other Augmin components HAUS6 (GTX118732), HAUS2 (GTX118734) and HAUS1 (GTX118733) in U2OS cells. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

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GTX113617 WB Image

GFP antibody detects GFP protein by western blot analysis.
A. 1 µg 293T whole cell extract
B. 1 µg 293T whole cell extract expressing GFP-tagged protein
C. 1 µg 293T whole cell extract expressing RFP-tagged protein
12% SDS-PAGE
GFP antibody (GTX113617) dilution: 1:10000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

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GTX113617 ICC/IF Image

Immunofluorescence analysis of GFP-transfected HeLa. A:GFP is expressed in the tranfected cell. B: The cell expressing GFP can be detected using rabbit anti-GFP ab ( GTX113617, 1:5000) followed by Alexa Fluor 594 (1:500) goat anti-rabbit IgG. C: Merged with DNA probe, the lower cell with no GFP expressed as a negtive control.

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GTX113617 IP Image

GFP was immunoprecipitated from bacterial GFP-expressing lysate using 1 ug of anti-GFP antibody (GTX113617).
The precipitated GFP was detected by GTX113617 diluted at 1:15000.

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GTX113617 WB Image

GFP antibody detects GFP protein by western blot analysis. Non-transfected (-) and GFP-transfected (+, ) 293T whole cell extracts (1 µg) were separated by 12% SDS-PAGE, and the membrane was blotted with GFP antibody (GTX113617) at a dilution of 1:10000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

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GTX113617 IHC-Wm Image

Immunohistochemical analysis (whole mount) of transgenic zebrafish embryo, using GFP(GTX113617) antibody at 1:200 dilution.

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GTX113617 WB Image

IP-WB assay to show that Hice1 (GTX113617) co-immunoprecipitated with other Augmin components HAUS6 (GTX118732), HAUS2 (GTX118734) and HAUS1 (GTX118733) in U2OS cells. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

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GTX113617 WB Image

GFP antibody detects GFP protein by western blot analysis.
A. 1 µg 293T whole cell extract
B. 1 µg 293T whole cell extract expressing GFP-tagged protein
C. 1 µg 293T whole cell extract expressing RFP-tagged protein
12% SDS-PAGE
GFP antibody (GTX113617) dilution: 1:10000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Top

GTX113617 ICC/IF Image

Immunofluorescence analysis of GFP-transfected HeLa. A:GFP is expressed in the tranfected cell. B: The cell expressing GFP can be detected using rabbit anti-GFP ab ( GTX113617, 1:5000) followed by Alexa Fluor 594 (1:500) goat anti-rabbit IgG. C: Merged with DNA probe, the lower cell with no GFP expressed as a negtive control.

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GTX113617 IP Image

GFP was immunoprecipitated from bacterial GFP-expressing lysate using 1 ug of anti-GFP antibody (GTX113617).
The precipitated GFP was detected by GTX113617 diluted at 1:15000.

Top

GTX113617 WB Image

GFP antibody detects GFP protein by western blot analysis. Non-transfected (-) and GFP-transfected (+, ) 293T whole cell extracts (1 µg) were separated by 12% SDS-PAGE, and the membrane was blotted with GFP antibody (GTX113617) at a dilution of 1:10000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

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StarReview
GFP antibody
Rating : ★★★★★ ( Average 5.0 based on 4 users reviews)
Immunohistochemistry (IHC)(1) :★★★★★
Western Blot (WB)(3) :★★★★☆

Anonymous StarReview submitted on 22 Feb 2018
Score : ★★★★★
Application Tested : IHC
Sample : Drosophila Retinal tissue
Blocking conditions
Blocking Buffer : 10% NGS in 0.3% PBST (triton-X-100)
Incubation Time & Temperature : 25 (℃)/1 hr
Primary antibody
Dilution : 1:200( conc. of dilution )
Incubation Time & Temperature : 4 (℃)/overnight
Data Description
Green: GTX113617 GFP antibody: photoreceptor cell R8, Red: GTX630189 mcherry antibody: medulla

Anonymous StarReview submitted on 12 Apr 2012
Score : ★★★★★
Application Tested : WB
Sample Species : Arobidopsis thiliana
Sample : Protoplast from Rosette leaves
Amount used(ug/cells) : 30ug
Blocking conditions
Blocking Buffer : 5% milk in PBST
Incubation Time & Temperature : 4℃ (℃)/16hr
Primary antibody
Dilution : 1:10000( conc. of dilution )
Incubation Time & Temperature : RT (℃)/1hr

Anonymous StarReview submitted on 09 Oct 2014
Score : ★★★★☆
Application Tested : WB
Sample : GFP-p53 transfected U2OS cells
Amount used(ug/cells) : 15ug
Blocking conditions
Blocking Buffer : 5% milk in 0.1%TBST
Incubation Time & Temperature : 25 (℃)/0.5hr
Primary antibody
Dilution : 1:5000( conc. of dilution )
Incubation Time & Temperature : 25 (℃)/1hr
Data Description
A: U2OS cells transfected with GFP-p53 B: U2OS cells transfected with control plasmid (no GFP-tag)

Anonymous StarReview submitted on 09 Oct 2014
Score : ★★★★★
Application Tested : WB
Sample : GFP recombinant protein transfected 293T cells
Amount used(ug/cells) : 20ug
Blocking conditions
Blocking Buffer : 5% milk
Incubation Time & Temperature : RT (℃)/1hr
Primary antibody
Dilution : 1:5000( conc. of dilution )
Incubation Time & Temperature : 4 (℃)/overnight

Reference for GFP antibody

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Lai CW et al. Using Dual Fluorescence Reporting Genes to Establish an In Vivo Imaging Model of Orthotopic Lung Adenocarcinoma in Mice. Mol Imaging Biol (Epub ahead of print) (2016).	WB, IHC-P
Lai CW et al. Sexually Dimorphic Expression of eGFP Transgene in the Akr1A1 Locus of Mouse Liver Regulated by Sex Hormone-Related Epigenetic Remodeling. Sci Rep 6:24023 (2016).	WB, IHC-P
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Steinke KV et al. Human CLC-K Channels Require Palmitoylation of Their Accessory Subunit Barttin to Be Functional. J Biol Chem 290 (28):17390-400 (2015).	IP
Ross E et al. AMP-Activated Protein Kinase Regulates the Cell Surface Proteome and Integrin Membrane Traffic. PLoS One 10 (5):e0128013 (2015).	WB
Yu H et al. Autonomous and non-autonomous roles of DNase?II during cell death in C. elegans embryos. Biosci Rep 35 (3) (2015).	IHC-Wm
Yang FM et al. A calreticulin-dependent nuclear export signal is involved in the regulation of liver receptor homologue-1 protein folding. Biochem J 471 (2):199-209 (2015).	IP
Dickmeis C et al. Production of Hybrid Chimeric PVX Particles Using a Combination of TMV and PVX-Based Expression Vectors. Front Bioeng Biotechnol 3:189 (2015).	WB
Chen PJ et al. NPGPx modulates CPEB2-controlled HIF-1α RNA translation in response to oxidative stress. Nucleic Acids Res 43 (19):9393-404 (2015).
Li Q et al. Akt Phosphorylates Wnt Coactivator and Chromatin Effector Pygo2 at Serine 48 to Antagonize Its Ubiquitin/Proteasome-mediated Degradation. J Biol Chem 290 (35):21553-67 (2015).
Wu JM et al. Circulating cells contribute to cardiomyocyte regeneration after injury. Circ Res 116 (4):633-41 (2015).	IHC-P
Lee WY et al. Repositioning antipsychotic chlorpromazine for treating colorectal cancer by inhibiting sirtuin 1. Oncotarget (Epub Ahead of Print) (2015).	WB
Chen CJ et al. SH2B1 and IRSp53 Proteins Promote the Formation of Dendrites and Dendritic Branches. J Biol Chem 290 (10):6010-21 (2015).	ICC/IF, WB
Wu HC et al. Production and characterization of active recombinant interleukin-12/eGFP fusion protein in stably-transfected DF1 chicken cells. Biotechnol Prog (2015).	ELISA
Auer TO et al. Highly efficient CRISPR/Cas9-mediated knock-in in zebrafish by homology-independent DNA repair. Genome Res 24 (1):142-53 (2014).	IHC-Wm
Hsueh YC et al. Prostaglandin E? promotes post-infarction cardiomyocyte replenishment by endogenous stem cells. EMBO Mol Med 6 (4):496-503 (2014).	IHC-P
Willemen HL et al. Monocytes/Macrophages control resolution of transient inflammatory pain. J Pain 15 (5):496-506 (2014).	IHC-Fr
Hong JB et al. A nucleolus-predominant piggyBac transposase, NP-mPB, mediates elevated transposition efficiency in mammalian cells. PLoS One 9 (2):e89396 (2014).	WB
Pope-Varsalona H et al. Polymerase η suppresses telomere defects induced by DNA damaging agents. Nucleic Acids Res 42 (21):13096-109 (2014).	ICC/IF
Huang IH et al. GEF-H1 controls focal adhesion signaling that regulates mesenchymal stem cell lineage commitment. J Cell Sci. 127 (Pt 19):4186-200 (2014).	WB
Yu Z et al. Using a yeast two-hybrid system to identify FTCD as a new regulator for HIF-1α in HepG2 cells. Cell Signal 26 (7):1560-1566 (2014).	WB
S Shukla et al. Molecular farming of fluorescent virus-based nanoparticles for optical imaging in plants, human cells and mouse models Biomater Sci (2014).
Hsu CY et al. The Epstein-Barr Virus-Encoded MicroRNA MiR-BART9 Promotes Tumor Metastasis by Targeting E-Cadherin in Nasopharyngeal Carcinoma. PLoS Pathog 10 (2):e1003974 (2014).	IHC
Chang YJ et al. SH2B1β Interacts with STAT3 and Enhances Fibroblast Growth Factor 1-Induced Gene Expression during Neuronal Differentiation. Mol Cell Biol 34 (6):1003-19 (2014).	IP, WB
Molleken K et al. The Chlamydia pneumoniae invasin protein Pmp21 recruits the EGF receptor for host cell entry. PLoS Pathog 9 (4):e1003325 (2013).
Reetz J et al. Development of Adenoviral Delivery Systems to Target Hepatic Stellate Cells In Vivo. PLoS One 8 (6):e67091 (2013).	IHC-P
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Barak LS et al. Triphenylmethane dye activation of beta-arrestin. Biochemistry 52 (32):5403-14 (2013).
Chang RY et al. Japanese encephalitis virus non-coding RNA inhibits activation of interferon by blocking nuclear translocation of interferon regulatory factor 3. Vet Microbiol 166 (1-2):11-21 (2013).	IP
Park J et al. RAS-MAPK-MSK1 pathway modulates ataxin 1 protein levels and toxicity in SCA1. Nature 498 (7454):325-31 (2013).	WB
Wang H et al. Balancing GRK2 and EPAC1 levels prevents and relieves chronic pain. J Clin Invest 123 (12):5023-34 (2013).	IHC-Fr
He G et al. Identification of liver cancer progenitors whose malignant progression depends on autocrine IL-6 signaling. Cell 155 (2):384-96 (2013).	IHC-P
Lin LT et al. mPlum-IFP 1.4 fluorescent fusion protein may display Förster resonance energy transfer associated properties that can be used for near-infrared based reporter gene imaging. J Biomed Opt 18 (12):126013 (2013).	WB
Hsu WB et al. Cellular protein HAX1 interacts with the influenza A virus PA polymerase subunit and impedes its nuclear translocation. J Virol 87 (1):110-23 (2013).	WB
Chiang YJ et al. CBAP functions as a novel component in chemokine-induced ZAP70-mediated T-cell adhesion and migration. PLoS One 8 (4):e61761 (2013).	WB
Tsou TC et al. Crucial role of Toll-like receptors in the zinc/nickel-induced inflammatory response in vascular endothelial cells. Toxicol Appl Pharmacol 273 (3):492-9 (2013).	WB
Wei PC et al. Loss of the oxidative stress sensor NPGPx compromises GRP78 chaperone activity and induces systemic disease. Mol Cell 48 (5):747-59 (2012).	WB
Hsiao WC et al. Vitamin D3-inducible mesenchymal stem cell-based delivery of conditionally replicating adenoviruses effectively targets renal cell carcinoma and inhibits tumor growth. Mol Pharm 9 (5):1396-408 (2012).	IHC
Lai SL et al. Autotaxin/Lpar3 signaling regulates Kupffer's vesicle formation and left-right asymmetry in zebrafish. Development 139 (23):4439-48 (2012).
Kwanchanok S et al. In vitro fluorescent analysis of preprotein import into chloroplasts. Biosci Biotechnol Biochem 75 (10):2001-7 (2011).	WB