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GNAS antibody

Anti-GNAS antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX32629
Anti-GNAS antibody used in Immunoprecipitation (IP). GTX32629
Anti-GNAS antibody used in Western Blot (WB). GTX32629
Anti-GNAS antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX32629

Cat. No. GTX32629

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, ICC/IF, IP

Reactivity

Human, Mouse, Rat
Package
100 μl ($399)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500 - 1:2000
ICC/IF 1:50 - 1:200
IP 1:50 - 1:200
Not tested in other applications.

PROPERTIES

Form

Liquid

Buffer

PBS, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 1-394 of human GNAS (NP_000507.1).

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

GNAS complex locus , AHO , C20orf45 , GNAS1 , GPSA , GSA , GSP , NESP , PITA3 , POH , SCG6 , SgVI

Cellular Localization

Cell membrane,Apical cell membrane,Secreted

Background

This locus has a highly complex imprinted expression pattern. It gives rise to maternally, paternally, and biallelically expressed transcripts that are derived from four alternative promoters and 5' exons. Some transcripts contain a differentially methylated region (DMR) at their 5' exons, and this DMR is commonly found in imprinted genes and correlates with transcript expression. An antisense transcript is produced from an overlapping locus on the opposite strand. One of the transcripts produced from this locus, and the antisense transcript, are paternally expressed noncoding RNAs, and may regulate imprinting in this region. In addition, one of the transcripts contains a second overlapping ORF, which encodes a structurally unrelated protein - Alex. Alternative splicing of downstream exons is also observed, which results in different forms of the stimulatory G-protein alpha subunit, a key element of the classical signal transduction pathway linking receptor-ligand interactions with the activation of adenylyl cyclase and a variety of cellular reponses. Multiple transcript variants encoding different isoforms have been found for this gene. Mutations in this gene result in pseudohypoparathyroidism type 1a, pseudohypoparathyroidism type 1b, Albright hereditary osteodystrophy, pseudopseudohypoparathyroidism, McCune-Albright syndrome, progressive osseus heteroplasia, polyostotic fibrous dysplasia of bone, and some pituitary tumors. [provided by RefSeq, Aug 2012]

Database

Research Area

DATA IMAGES

Anti-GNAS antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX32629

GTX32629 ICC/IF Image

ICC/IF analysis of L929 cells using GTX32629 GNAS antibody.
Blue : DAPI
Dilution : 1:100

Anti-GNAS antibody used in Immunoprecipitation (IP). GTX32629

GTX32629 IP Image

IP analysis of mouse brain tissue lysate using GTX32629 GNAS antibody.
Antibody amount : 3μg / 200μg lysate
Dilution : 1:1000

Anti-GNAS antibody used in Western Blot (WB). GTX32629

GTX32629 WB Image

WB analysis of various sample lysates using GTX32629 GNAS antibody.
Dilution : 1:1000
Loading : 25μg per lane

Anti-GNAS antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX32629

GTX32629 ICC/IF Image

ICC/IF analysis of U2OS cells using GTX32629 GNAS antibody.
Blue : DAPI
Dilution : 1:100

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399