GRB10 (phospho Tyr67) antibody

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GTX32187

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100 μl	USD 319.00

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Catalog Number	GTX32187 ☆☆☆☆☆ ( 0 ) ( 0 )
Product Name	GRB10 (phospho Tyr67) antibody See all GRB10 products
Applications	ICC/IF, WB
Application Note	WB: 1:500 - 1:1000. ICC/IF: 1:100 - 1:500
Form Supplied	Liquid
Purification	The antibody was purified by immunogen affinity chromatography.

Full Name	growth factor receptor-bound protein 10
Background	The product of this gene belongs to a small family of adapter proteins that are known to interact with a number of receptor tyrosine kinases and signaling molecules. This gene encodes a growth factor receptor-binding protein that interacts with insulin receptors and insulin-like growth-factor receptors. Overexpression of some isoforms of the encoded protein inhibits tyrosine kinase activity and results in growth suppression. This gene is imprinted in a highly isoform- and tissue-specific manner, with expression observed from the paternal allele in the brain, and from the maternal allele in the placental trophoblasts. Alternatively spliced transcript variants encoding different isoforms have been identified. [provided by RefSeq, Oct 2010]
Synonyms	Grb-10 Antibody , GRB-IR Antibody , IRBP Antibody , RSS Antibody , MEG1 Antibody , GRB10 Antibody , growth factor receptor-bound protein 10 Antibody
Specificity	Recognizes endogenous levels of GRB10 (pY67) protein.
Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Immunogen	KLH-conjugated synthetic peptide encompassing a sequence within the N-term region of human GRB10. The exact sequence is proprietary.
Antigen Species	Human
Species Reactivity	Human, Mouse, Rat
Conjugation	Unconjugated

Storage Buffer	Liquid in 0.42% Potassium phosphate; 0.87% Sodium chloride; pH 7.3; 30% glycerol; and 0.01% sodium azide.
Storage Instruction	Shipped at 4 °C. Upon delivery aliquot and store at -20 °C for one year. Avoid freeze/thaw cycles.
Notes	For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
ResearchArea
Disease Related > Diabetes Immunology > Autoimmunity > Autoimmune diseases > Diabetes More Hide

GRB10 (phospho Tyr67) antibody validated data

GTX32187 WB Image

Western blot analysis of GRB10 (pY67) expression in HEK293T insulin-treated (A); mouse spleen (B); PC12 insulin-treated (C) whole cell lysates.

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GTX32187 ICC/IF Image

Immunofluorescent analysis of GRB10 (pY67) staining in PC12 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).

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GTX32187 WB Image

Western blot analysis of GRB10 (pY67) expression in HEK293T insulin-treated (A); mouse spleen (B); PC12 insulin-treated (C) whole cell lysates.

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GTX32187 ICC/IF Image

Immunofluorescent analysis of GRB10 (pY67) staining in PC12 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).

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Primary Antibodies