|Applications||ICC/IF, IHC-P, WB|
Not tested in other applications.
|IHC (Formalin-fixed paraffin-embedded sections)||1:100-1:1000*
*Optimal dilutions/concentrations should be determined by the researcher.
|Predicted Target Size||25 kDa (note)
|Positive Controls||293T , A431 , H1299 , HeLa , HepG2 , Molt-4 , Raji , NIH-3T3 , JC , BCL-1|
(Please refer to the vial label for the specific concentration)|
|Purification||Purified by antigen-affinity chromatography.|
|Full Name||growth factor receptor-bound protein 2|
|Product Description||Rabbit Polyclonal antibody to GRB2 (growth factor receptor-bound protein 2)|
|Background||The protein encoded by this gene binds the epidermal growth factor receptor and contains one SH2 domain and two SH3 domains. Its two SH3 domains direct complex formation with proline-rich regions of other proteins, and its SH2 domain binds tyrosine phosphorylated sequences. This gene is similar to the Sem5 gene of C.elegans, which is involved in the signal transduction pathway. Two alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq]|
|Synonyms||ASH antibody, EGFRBP-GRB2 antibody, Grb3-3 antibody, MST084 antibody, MSTP084 antibody, NCKAP2 antibody, GRB2 antibody, protein Ash antibody, growth factor receptor-bound protein 3 antibody, SH2/SH3 adapter GRB2 antibody, abundant SRC homology antibody, growth factor receptor-bound protein 2 antibody, epidermal growth factor receptor-binding protein GRB2 antibody, HT027 antibody|
|Cellular Localization||Golgi apparatus |
|Immunogen||Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human GRB2. The exact sequence is proprietary.|
|Species Reactivity||Human, Mouse, Rat|
|Predicted Cross Reactivity species Predicted cross-reactivity:|
Predicted cross-reactivity is based on sequence homology, with greater than 80% immunogen sequence identity considered positive.
Please note that we are only able to guarantee products to work in applications and species in which they have been tested.
|Pig, Chimpanzee, Bovine|
|Predict Reactivity Note||Pig (100%), Chimpanzee (100%), Bovine (100%)|