|Applications||ICC/IF, IHC-P, IP, WB|
|IHC (Formalin-fixed paraffin-embedded sections)||1:100-1:1000*
IHC (Formalin-fixed paraffin-embedded sections) : suggested antigen retrieval using heat mediated 10mM Citrate buffer (pH6.0) or Tris-EDTA buffer (pH8.0)Not tested in other applications.
*Optimal dilutions/concentrations should be determined by the researcher.
|Predicted Target Size||92 kDa (note)
|Positive Controls||293T , A431 , HeLa , HepG2 , NIH-3T3 , Mouse brain , PC-12 , Rat-2|
(Please refer to the vial label for the specific concentration)|
|Purification||Purified by antigen-affinity chromatography.|
|Full Name||heat shock protein 90kDa beta (Grp94), member 1|
|Product Description||Rabbit Polyclonal antibody to GRP94 (heat shock protein 90kDa beta (Grp94), member 1)|
|Background||HSP90 proteins are highly conserved molecular chaperones that have key roles in signal transduction, protein folding, protein degradation, and morphologic evolution. HSP90 proteins normally associate with other cochaperones and play important roles in folding newly synthesized proteins or stabilizing and refolding denatured proteins after stress. HSP90B1 is an endoplasmic reticulum HSP90 protein. Other HSP90 proteins are found in cytosol (see HSP90AA1; MIM 140571) and mitochondria (TRAP1; MIM 606219) (Chen et al., 2005 [PubMed 16269234]).[supplied by OMIM]|
|Synonyms||ECGP antibody, GP96 antibody, GRP94 antibody, TRA1 antibody, HSP90B1 antibody, endothelial cell (HBMEC) glycoprotein antibody, tumor rejection antigen 1 antibody, GRP-94 antibody, tumor rejection antigen (gp96) 1 antibody, Tumor rejection antigen-1 (gp96) antibody, 94 kDa glucose-regulated protein antibody, heat shock protein 90 kDa beta member 1 antibody, "glucose regulated protein, 94 kDa antibody", endoplasmin antibody, gp96 homolog antibody, "heat shock protein 90kDa beta (Grp94), member 1 antibody"|
|Cellular Localization||Endoplasmic reticulum lumen , Melanosome |
|Immunogen||Recombinant protein encompassing a sequence within the C-terminus region of human GRP94. The exact sequence is proprietary.|
|Species Reactivity||Human, Mouse, Rat|
|Predicted Cross Reactivity species Predicted cross-reactivity:|
Predicted cross-reactivity is based on sequence homology, with greater than 80% immunogen sequence identity considered positive.
Please note that we are only able to guarantee products to work in applications and species in which they have been tested.
|Dog, Monkey, Pig|
|Predict Reactivity Note||Dog (95%), Monkey (98%), Pig (95%)|