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GSTP1 antibody

Anti-GSTP1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX112953
Anti-GSTP1 antibody used in Western Blot (WB). GTX112953
Anti-GSTP1 antibody used in Western Blot (WB). GTX112953
Anti-GSTP1 antibody used in IHC (Paraffin sections) (IHC-P). GTX112953
Anti-GSTP1 antibody used in Western Blot (WB). GTX112953

Cat. No. GTX112953

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, ICC/IF, IHC-P

Reactivity

Human, Mouse, Rat
Package
100 μl ($399),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

23 kDa. ( Note )

Positive Control

Rat brain , HeLa , Molt-4 , Mouse brain

Predict Reactivity

Bovine, Dog, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.92 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human GSTP1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1950468

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

glutathione S-transferase pi 1 , DFN7 , FAEES3 , GST3 , GSTP , HEL-S-22 , PI

Background

Glutathione S-transferases (GSTs) are a family of enzymes that play an important role in detoxification by catalyzing the conjugation of many hydrophobic and electrophilic compounds with reduced glutathione. Based on their biochemical, immunologic, and structural properties, the soluble GSTs are categorized into 4 main classes: alpha, mu, pi, and theta. This GST family member is a polymorphic gene encoding active, functionally different GSTP1 variant proteins that are thought to function in xenobiotic metabolism and play a role in susceptibility to cancer, and other diseases. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-GSTP1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX112953

GTX112953 ICC/IF Image

GSTP1 antibody detects GSTP1 protein at cytoplasm and nucleus by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: GSTP1 stained by GSTP1 antibody (GTX112953) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

Anti-GSTP1 antibody used in Western Blot (WB). GTX112953

GTX112953 WB Image

Sample (50 μg of whole cell lysate)
A: mouse brain
12% SDS PAGE
GTX112953 diluted at 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-GSTP1 antibody used in Western Blot (WB). GTX112953

GTX112953 WB Image

GSTP1 antibody detects GSTP1 protein by western blot analysis.
A. 50 μg Rat brain lysate/extract
12% SDS-PAGE
GSTP1 antibody (GTX112953) dilution: 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-GSTP1 antibody used in IHC (Paraffin sections) (IHC-P). GTX112953

GTX112953 IHC-P Image

GSTP1 antibody detects GSTP1 protein at cytoplasm and nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded human breast carcinoma.
GSTP1 stained by GSTP1 antibody (GTX112953) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-GSTP1 antibody used in Western Blot (WB). GTX112953

GTX112953 WB Image

Various whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with GSTP1 antibody (GTX112953) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.

REFERENCE

REVIEW

GSTP1 antibody Cat. No. GTX112953

Rating ( Average 5 based on 1 users reviews)
Application
Western Blot(WB) ( Average 5 based on 1 users reviews)
Date : Anonymous submitted on 18-Mar-2021
Score :
Application Tested : WB
Sample Species : Hu
Figure Note : The data was peer reviewed and published in the journal Evid Based Complement Alternat Med in 2012. PMID: 22536285
SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169