ATG12 antibody

Name: ATG12 antibody
Brand: GeneTex
SKU: GTX124181
Price: 399.00 USD

Anti-ATG12 antibody used in Western Blot (WB). GTX124181

Anti-ATG12 antibody used in Immunoprecipitation (IP). GTX124181

Anti-ATG12 antibody used in IHC (Paraffin sections) (IHC-P). GTX124181

Cat. No. GTX124181

Reference 14

Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Application	WB, ICC/IF, IHC-P, IP
Reactivity	Human, Mouse, Rat
Package	100 μl ($399), 25 μl ($169)

See all ATG12 products

View product citations for antibody GTX124181 on CiteAb

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Recommended Dilution
WB	1:500-1:3000
ICC/IF	Assay dependent
IHC-P	1:100-1:1000
IP	1:100-1:500

Not tested in other applications.

Calculated MW

15 kDa. ( Note )

Positive Control

NIH-3T3 , Rat brain , 293T

Predict Reactivity

Pig, Chimpanzee(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.43 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human ATG12. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_11171947

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

autophagy related 12 , APG12 , APG12L , FBR93 , HAPG12

Cellular Localization

Cytoplasm

Background

Autophagy is a process of bulk protein degradation in which cytoplasmic components, including organelles, are enclosed in double-membrane structures called autophagosomes and delivered to lysosomes or vacuoles for degradation. ATG12 is the human homolog of a yeast protein involved in autophagy (Mizushima et al., 1998 [PubMed 9852036]).[supplied by OMIM]

Database

Gene ID: 9140 ATG12
UniProt: O94817 ATG12

Research Area

DATA IMAGES

GTX124181 WB Image

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with ATG12 antibody (GTX124181) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX124181 WB Image

Sample (30 μg of whole cell lysate)
A: NIH-3T3
12% SDS PAGE
GTX124181 diluted at 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX124181 IP Image

Immunoprecipitation of Atg 12-Atg5 protein from 293T whole cell extracts using 5 μg of ATG12 antibody (GTX124181).
Western blot analysis was performed using ATG12 antibody (GTX124181) diluted at 1:1000.
EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.

GTX124181 IHC-P Image

ATG12 antibody detects ATG12 protein at cytoplasm in rat brain by immunohistochemical analysis.
Sample: Paraffin-embedded rat brain.
ATG12 antibody (GTX124181) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

GTX124181 IHC-P Image

ATG12 antibody detects ATG12 protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded human lung cancer.
ATG12 stained by ATG12 antibody (GTX124181) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

GTX124181 WB Image

Sample (50 μg of whole cell lysate)
A: Rat brain
12% SDS PAGE
GTX124181 diluted at 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX124181 WB Image

Various whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with ATG12 antibody (GTX124181) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.