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Goat Anti-Rabbit IgG antibody (HRP)

Goat Anti-Rabbit IgG antibody (HRP). GTX213110-01
Goat Anti-Rabbit IgG antibody (HRP). GTX213110-01
Goat Anti-Rabbit IgG antibody (HRP). GTX213110-01
Goat Anti-Rabbit IgG antibody (HRP). GTX213110-01
Goat Anti-Rabbit IgG antibody (HRP). GTX213110-01

Cat. No. GTX213110-01

Host

Goat

Clonality

Polyclonal

Isotype

IgG

Application

WB, IHC-P, ELISA

Reactivity

Rabbit
Package
1 ml ($149)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB Assay dependent
IHC-P 1:100-1:1000
ELISA Assay dependent
Not tested in other applications.

PROPERTIES

Form

Liquid

Buffer

0.05M Tris, 0.15M NaCl, 1%BSA

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.5 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Rabbit

Immunogen

Highly purified whole rabbit IgG

Target Isotype

IgG

Purification

Purified by antigen-affinity chromatography.

Conjugation

Horseradish peroxidase(HRP)

RRID

AB_10618573

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

antiRabbit IgG antibody , anti-Rabbit IgG antibody , Goat antiRabbit IgG HRP , Rabbit IgG secondary antibody , Goat anti-Rabbit IgG HRP , anti Rabbit IgG antibody , Goat anti Rabbit IgG HRP

Background

Immunoglobulin G (IgG), is one of the most abundant proteins in serum with normal levels between 8-17 mg/ml in adult blood. IgG is important for our defence against microorganisms and the molecules are produced by B lymphocytes as a part of our adaptive immune response. The IgG molecule has two separate functions; to bind to the pathogen that elicited the response and to recruit other cells and molecules to destroy the antigen. The variability of the IgG pool is generated by somatic recombination and the number of specificities in an individual at a given time point is estimated to be 1011 variants.

DATA IMAGES

Goat Anti-Rabbit IgG antibody (HRP). GTX213110-01

GTX213110-01 WB Image

Various whole cell extracts were separated by 10% SDS-PAGE, and the membrane was blotted with Rabbit IgG antibody (HRP) (GTX213110-01) diluted at 1:10000.

Goat Anti-Rabbit IgG antibody (HRP). GTX213110-01

GTX213110-01 ELISA Image

Indirect ELISA analysis performed by coating plate with recombinant SARS-CoV-2 (COVID-19) Spike S1 protein, His tag (active) (GTX135817-pro) (52.15-0.81 nM). Coated protein was probed with SARS-CoV-2 (COVID-19) Spike S1 antibody [HL134] (GTX635671) (1 μg/mL). Goat anti-rabbit IgG antibody (HRP) (GTX213110-01) (1:10000) was used to detect bound primary antibody.

Goat Anti-Rabbit IgG antibody (HRP). GTX213110-01

GTX213110-01 IHC-P Image

WBP11 antibody detects WBP11 protein at nucleus in mouse intestine by immunohistochemical analysis.
Sample: Paraffin-embeddedmouse intestine.
WBP11 antibody (GTX118654) diluted at 1:500.
The signal was developed byRabbit IgG antibody (HRP) (GTX213110-01)
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Goat Anti-Rabbit IgG antibody (HRP). GTX213110-01

GTX213110-01 WB Image

Various amounts of denatured rabbit IgG protein were separated by 12% SDS-PAGE, and the membrane was blotted with HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) diluted at 1:5000.

Goat Anti-Rabbit IgG antibody (HRP). GTX213110-01

GTX213110-01 IHC-P Image

WBP11 antibody detects WBP11 protein at nucleus in rat prostate by immunohistochemical analysis.
Sample: Paraffin-embedded rat prostate.
WBP11 antibody (GTX118654) diluted at 1:500.
The signal was developed by Rabbit IgG antibody (HRP) (GTX213110-01).
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

REFERENCE

REVIEW

Goat Anti-Rabbit IgG antibody (HRP) Cat. No. GTX213110-01

Rating ( Average 5 based on 14 users reviews)
Application
Western Blot(WB) ( Average 5 based on 14 users reviews)
Date : Anonymous submitted on 24-Jul-2023
Score :
Application Tested : WB
Figure Note : The data was peer reviewed and published in the 2022 in Cancers (Basel). PMID: 35267540
Date : Anonymous submitted on 03-Oct-2022
Score :
Application Tested : WB
Figure Note : The data was peer reviewed and published in the 2022 in Front Aging Neurosci. PMID: 35264943
Date : Anonymous submitted on 03-Oct-2022
Score :
Application Tested : WB
Figure Note : The data was peer reviewed and published in the 2017 in Nat Commun. PMID: 28281525
Date : Anonymous submitted on 03-Oct-2022
Score :
Application Tested : WB
Figure Note : The data was peer reviewed and published in the 2017 in Nat Commun. PMID: 28281525
Date : Anonymous submitted on 03-Oct-2022
Score :
Application Tested : WB
Figure Note : The data was peer reviewed and published in the 2017 in Nat Commun. PMID: 28281525
Date : Anonymous submitted on 03-Oct-2022
Score :
Application Tested : WB
Figure Note : The data was peer reviewed and published in the 2016 in PLoS One. PMID: 27486856
Date : Anonymous submitted on 10-Aug-2021
Score :
Application Tested : WB
Sample Species : Rb
Figure Note : The data was peer reviewed and published in the journal Cell Commun Signal in 2014.PMID: 25214434
Date : Anonymous submitted on 10-Aug-2021
Score :
Application Tested : WB
Sample Species : Rb
Figure Note : The data was peer reviewed and published in the journal PLoS One in 2017.PMID: 28832634
Date : Anonymous submitted on 10-Aug-2021
Score :
Application Tested : WB
Sample Species : Rb
Figure Note : The data was peer reviewed and published in the journal Front Pharmacol in 2018.PMID: 30087612
Date : Anonymous submitted on 18-Mar-2021
Score :
Application Tested : WB
Figure Note : The data was peer reviewed and published in the journal PLoS One in 2017. PMID: 28945763
Date : Anonymous submitted on 18-Mar-2021
Score :
Application Tested : WB
Figure Note : The data was peer reviewed and published in the journal Oncoscience in 2015. PMID: 26097885
Date : Anonymous submitted on 18-Mar-2021
Score :
Application Tested : WB
Sample Species : Rb
Figure Note : The data was peer reviewed and published in the journal EMBO Rep in 2020. PMID: 31885126
Date : Anonymous submitted on 18-Mar-2021
Score :
Application Tested : WB
Sample Species : Rb
Figure Note : The data was peer reviewed and published in the journal EMBO Rep in 2020. PMID: 31885126
Date : Anonymous submitted on 18-Mar-2021
Score :
Application Tested : WB
Sample Species : Rb
Figure Note : The data was peer reviewed and published in the journal EMBO Rep in 2020. PMID: 31885126
Application Reference
SDS
Proclin.pdf
Package List Price ($)
$ 149