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Galectin 7 antibody

Anti-Galectin 7 antibody used in Western Blot (WB). GTX121475
Anti-Galectin 7 antibody used in Western Blot (WB). GTX121475
Anti-Galectin 7 antibody used in Immunoprecipitation (IP). GTX121475

Cat. No. GTX121475

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IP

Reactivity

 Human
Package
100 μl ($399),
25 μl ($169)
See all Galectin 7 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
IP 1:100-1:500
Not tested in other applications.

Calculated MW

15 kDa. ( Note )

Positive Control

LGALS7 transfected 293T

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.54 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Full length human Galectin 7 Recombinant protein.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_10721181

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

galectin 7 , GAL7 , LGALS7A

Cellular Localization

Cytoplasm , Nucleus , Secreted (Potential)

Background

The galectins are a family of beta-galactoside-binding proteins implicated in modulating cell-cell and cell-matrix interactions. Differential and in situ hybridization studies indicate that this lectin is specifically expressed in keratinocytes and found mainly in stratified squamous epithelium. A duplicate copy of this gene (GeneID:653499) is found adjacent to, but on the opposite strand on chromosome 19. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-Galectin 7 antibody used in Western Blot (WB). GTX121475

GTX121475 WB Image

Western blot experiment using Galectin 7 Antibody (GTX121475) at 1:90000 dilution to detect 100ng of 18kDa recombinant Galectin 7 protein antigen.

Anti-Galectin 7 antibody used in Western Blot (WB). GTX121475

GTX121475 WB Image

Sample (30 ug of whole cell lysate)
A: Non-transfected 293T lysates
B: LGALS7 transfected 293T lysates
15% SDS PAGE
GTX121475 diluted at 1:1000

Anti-Galectin 7 antibody used in Immunoprecipitation (IP). GTX121475

GTX121475 IP Image

Galectin 7 antibody immunoprecipitates Galectin 7 protein in IP experiments.
IP samples: LGALS7 transfected 293T lysates
A. Control with 5 μg of preimmune Rabbit IgG
B. Immunoprecipitation of Galectin 7 protein by 5 μg Galectin 7 antibody (GTX121475)
10 % SDS-PAGE
The immunoprecipitated Galectin 7 protein was detected by Galectin 7 antibody (GTX121475) diluted at 1:500.
[EasyBlot anti-rabbit IgG (GTX221666-01) was used as a secondary reagent]

REFERENCE

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REVIEW

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169