|Applications||ICC/IF, IHC-P, WB|
Not tested in other applications.
|IHC (Formalin-fixed paraffin-embedded sections)||1:100-1:1000*
*Optimal dilutions/concentrations should be determined by the researcher.
|Predicted Target Size||26 kDa (note)
|Positive Controls||A549 , H1299 , HCT116 , MCF-7 , Recombinant LGALS3 protein , rat colon|
(Please refer to the vial label for the specific concentration)|
|Purification||Purified by antigen-affinity chromatography.|
|Full Name||lectin, galactoside-binding, soluble, 3|
|Product Description||Rabbit Polyclonal antibody to Galectin3 (galectin-3)|
|Background||This gene encodes a member of the galectin family of carbohydrate binding proteins. Members of this protein family have an affinity for beta-galactosides. The encoded protein is characterized by an N-terminal proline-rich tandem repeat domain and a single C-terminal carbohydrate recognition domain. This protein can self-associate through the N-terminal domain allowing it to bind to multivalent saccharide ligands. This protein localizes to the extracellular matrix, the cytoplasm and the nucleus. This protein plays a role in numerous cellular functions including apoptosis, innate immunity, cell adhesion and T-cell regulation. Alternate splicing results in multiple transcript variants.|
|Synonyms||CBP35 antibody, GAL3 antibody, GALBP antibody, GALIG antibody, L31 antibody, LGALS2 antibody, MAC2 antibody, LGALS3 antibody, IgE-binding protein antibody, carbohydrate-binding protein 35 antibody, 35 kDa lectin antibody, MAC-2 antigen antibody, galactose-specific lectin 3 antibody, galectin-3 antibody, laminin-binding protein antibody, lectin L-29 antibody, "lectin, galactoside-binding, soluble, 3 antibody"|
|Cellular Localization||Nucleus , Cytoplasm , Secreted|
|Immunogen||Recombinant protein encompassing a sequence within the center region of human Galectin3. The exact sequence is proprietary.|
|Species Reactivity||Human, Rat|
|Predicted Cross Reactivity species Predicted cross-reactivity:|
Predicted cross-reactivity is based on sequence homology, with greater than 80% immunogen sequence identity considered positive.
Please note that we are only able to guarantee products to work in applications and species in which they have been tested.
|Mouse, Dog, Pig, Rhesus Monkey|
|Predict Reactivity Note||Mouse (81%), Dog (84%), Pig (81%), Rhesus Monkey (91%)|