Glucokinase antibody [N1C1-2]
|Applications||ICC/IF, IHC-P, WB|
Not tested in other applications.
|IHC (Formalin-fixed paraffin-embedded sections)||1:100-1:1000*
*Optimal dilutions/concentrations should be determined by the researcher.
|Predicted Target Size||52 kDa (note)
|Positive Controls||293T , A431 , H1299 , HeLa , HepG2 , Molt-4 , Raji , Huh7 , Hep3B , HepG2|
(Please refer to the vial label for the specific concentration)|
|Purification||Purified by antigen-affinity chromatography.|
|Full Name||glucokinase (hexokinase 4)|
|Product Description||Rabbit Polyclonal antibody to Glucokinase (glucokinase (hexokinase 4))|
|Background||Hexokinases phosphorylate glucose to produce glucose-6-phosphate, thus committing glucose to the glycolytic pathway. Alternative splicing of this gene results in three tissue-specific forms of glucokinase, one found in pancreatic islet beta cells and two found in liver. The protein localizes to the outer membrane of mitochondria. In contrast to other forms of hexokinase, this enzyme is not inhibited by its product glucose-6-phosphate but remains active while glucose is abundant. Mutations in this gene have been associated with non-insulin dependent diabetes mellitus (NIDDM), maturity onset diabetes of the young, type 2 (MODY2) and persistent hyperinsulinemic hypoglycemia of infancy (PHHI). [provided by RefSeq]|
|Synonyms||FGQTL3 antibody, GK antibody, GLK antibody, HHF3 antibody, HK4 antibody, HKIV antibody, HXKP antibody, LGLK antibody, MODY2 antibody, GCK antibody, hexokinase-4 antibody, ATP:D-hexose 6-phosphotransferase antibody, hexokinase type IV antibody, hexokinase-D antibody, glucokinase antibody, "hexokinase D, pancreatic isozyme antibody", HK IV antibody, glucokinase (hexokinase 4) antibody|
|Immunogen||Recombinant protein encompassing a sequence within the center region of human Glucokinase. The exact sequence is proprietary.|
|Predicted Cross Reactivity species Predicted cross-reactivity:|
Predicted cross-reactivity is based on sequence homology, with greater than 80% immunogen sequence identity considered positive.
Please note that we are only able to guarantee products to work in applications and species in which they have been tested.
|Mouse, Rat, Zebrafish, Xenopus laevis, Cat, Sheep, Bovine|
|Predict Reactivity Note||Mouse (97%), Rat (97%), Zebrafish (86%), Xenopus laevis (87%), Cat (96%), Sheep (97%), Bovine (96%)|