Glutamate Dehydrogenase antibody
|Applications||ELISA, ICC/IF, IHC-P, WB|
Not tested in other applications.
| ELISA||Assay-dependent dilution
|IHC (Formalin-fixed paraffin-embedded sections)||1:100-1:1000*
*Optimal dilutions/concentrations should be determined by the researcher.
|Predicted Target Size||61 kDa (note)
|Positive Controls||293T , A431 , HeLa , HepG2 , *THP1 , *Raw264.7|
(Please refer to the vial label for the specific concentration)|
|Purification||Purified by antigen-affinity chromatography.|
|Full Name||glutamate dehydrogenase 1|
|Product Description||Rabbit Polyclonal antibody to Glutamate Dehydrogenase (glutamate dehydrogenase 1)|
|Background||L-glutamate dehydrogenase (EC 184.108.40.206) has a central role in nitrogen metabolism in plants and animals. Glutamate dehydrogenase is found in all organisms and catalyzes the oxidative deamination of 1-glutamate to 2-oxoglutarate (Smith et al., 2001 [PubMed 11254391]). Glutamate, the main substrate of GLUD, is present in brain in concentrations higher than in other organs. In nervous tissue, GLUD appears to function in both the synthesis and the catabolism of glutamate and perhaps in ammonia detoxification (Mavrothalassitis et al., 1988 [PubMed 3368458]).[supplied by OMIM]|
|Synonyms||GDH antibody, GDH1 antibody, GLUD antibody, MGC132003 antibody, GLUD1 antibody, "glutamate dehydrogenase 1, mitochondrial antibody", glutamate dehydrogenase (NAD(P)+) antibody, GDH 1 antibody, glutamate dehydrogenase 1 antibody|
|Cellular Localization||Mitochondrion matrix |
|Immunogen||Recombinant protein encompassing a sequence within the center region of human Glutamate Dehydrogenase. The exact sequence is proprietary.|
|Species Reactivity||Human, Mouse, Rat|
|Predicted Cross Reactivity species Predicted cross-reactivity:|
Predicted cross-reactivity is based on sequence homology, with greater than 80% immunogen sequence identity considered positive.
Please note that we are only able to guarantee products to work in applications and species in which they have been tested.
|Xenopus laevis, Pig, Sheep, Bovine|
|Predict Reactivity Note||Xenopus laevis (91%), Pig (99%), Sheep (99%), Bovine (98%)|