Glutamine synthetase antibody [GT1055]
|Applications||ICC/IF, IHC-Fr, IHC-P, WB|
Not tested in other applications.
|IHC (Frozen sections)||1:100-1:1000*
|IHC (Formalin-fixed paraffin-embedded sections)||1:100-1:1000*
*Optimal dilutions/concentrations should be determined by the researcher.
|Predicted Target Size||42 kDa (note)
|Positive Controls||293T , HeLa , HepG2 , U87-MG , SK-N-SH , IMR32 , SK-N-AS , mouse brain , mouse eye , rat brain|
(Please refer to the vial label for the specific concentration)|
|Purification||Affinity purified by Protein G.|
|Full Name||glutamate-ammonia ligase|
|Product Description||Mouse Monoclonal antibody [GT1055] to Glutamine synthetase (glutamate-ammonia ligase)|
|Background||Glutamine is a main source of energy and is involved in cell proliferation, inhibition of apoptosis, and cell signaling (Haberle et al., 2005 [PubMed 16267323]). Fetal glutamine requirements are very high and depend largely on active glutamine synthesis and the release of glutamine into the fetal circulation by the placenta. Glutamine synthetase (EC 22.214.171.124), also called glutamate-ammonia ligase (GLUL), is expressed throughout the body and plays an important role in controlling body pH and in removing ammonia from the circulation. The enzyme clears L-glutamate, the major neurotransmitter in the central nervous system, from neuronal synapses (see references in Clancy et al., 1996 [PubMed 8975719]).[supplied by OMIM]|
|Synonyms||GLNS Antibody, GS Antibody, PIG59 Antibody, PIG43 Antibody, glutamate-ammonia ligase Antibody, GLUL Antibody|
|Cellular Localization||Cytoplasm |
|Immunogen||Full length human Glutamine synthetase Recombinant protein.|
|Species Reactivity||Human, Mouse, Rat|
|Predicted Cross Reactivity species Predicted cross-reactivity:|
Predicted cross-reactivity is based on sequence homology, with greater than 80% immunogen sequence identity considered positive.
Please note that we are only able to guarantee products to work in applications and species in which they have been tested.
|Xenopus laevis, Dog, Pig, Chicken, Rhesus Monkey, Chimpanzee, Bovine|
|Predict Reactivity Note||Xenopus laevis (85%), Dog (97%), Pig (96%), Chicken (88%), Rhesus Monkey (100%), Chimpanzee (99%), Bovine (96%)|