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Glutathione S-transferase kappa 1 antibody [N1C2]

KO/KD
Anti-Glutathione S-transferase kappa 1 antibody [N1C2] used in Western Blot (WB). GTX119447
Anti-Glutathione S-transferase kappa 1 antibody [N1C2] used in IHC (Paraffin sections) (IHC-P). GTX119447
Anti-Glutathione S-transferase kappa 1 antibody [N1C2] used in IHC (Paraffin sections) (IHC-P). GTX119447
Anti-Glutathione S-transferase kappa 1 antibody [N1C2] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX119447

Cat. No. GTX119447

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human
Package
100 μl ($399),
25 μl ($169)
See all Glutathione S-transferase kappa 1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

25 kDa. ( Note )

Positive Control

293T

Predict Reactivity

Pig, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.85 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human Glutathione S-transferase kappa 1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_10633163

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

glutathione S-transferase kappa 1 , GST , GST 13-13 , GST13 , GST13-13 , GSTK1-1 , hGSTK1

Cellular Localization

Peroxisome

Background

This gene encodes a member of the kappa class of the glutathione transferase superfamily of enzymes that function in cellular detoxification. The encoded protein is localized to the peroxisome and catalyzes the conjugation of glutathione to a wide range of hydrophobic substates facilitating the removal of these compounds from cells. Alternative splicing results in multiple transcript variants.

Database

Research Area

DATA IMAGES

Anti-Glutathione S-transferase kappa 1 antibody [N1C2] used in Western Blot (WB). GTX119447

GTX119447 WB Image

KO/KD

Wild-type (WT) and Glutathione S-transferase kappa 1 knockout (KO) 293T cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with Glutathione S-transferase kappa 1 antibody (GTX119447) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Glutathione S-transferase kappa 1 antibody [N1C2] used in IHC (Paraffin sections) (IHC-P). GTX119447

GTX119447 IHC-P Image

Glutathione S-transferase kappa 1 antibody [N1C2] detects Glutathione S-transferase kappa 1 protein at peroxisome on human lung carcinoma by immunohistochemical analysis.
Sample: Paraffin-embedded lung carcinoma.
Glutathione S-transferase kappa 1 antibo
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-Glutathione S-transferase kappa 1 antibody [N1C2] used in IHC (Paraffin sections) (IHC-P). GTX119447

GTX119447 IHC-P Image

Immunohistochemical analysis of paraffin-embedded human gastric cancer, using Glutathione S-transferase kappa 1(GTX119447) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-Glutathione S-transferase kappa 1 antibody [N1C2] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX119447

GTX119447 ICC/IF Image

Immunofluorescence analysis of methanol-fixed A549, using Glutathione S-transferase kappa 1(GTX119447) antibody at 1:500 dilution.

REFERENCE

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REVIEW

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169