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1 .GTX130732 Glypican-3 antibody

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Glypican-3 antibody

Biological & Orthogonal

  • Host / ClonalityRabbit Polyclonal
  • ApplicationsICC/IF, WB
  • Species reactivityHuman
Cat No: GTX130732
Package Price Qty
25 μlUSD 119.00
100 μlUSD 319.00
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  • Anti-Glypican-3 antibody used in Western Blot (WB). GTX130732
  • Anti-Glypican-3 antibody used in Western Blot (WB). GTX130732
  • Anti-Glypican-3 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX130732
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  • Bio-info
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  • Application Information: Glypican-3 antibody

Catalog Number GTX130732
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Product Name Glypican-3 antibody
See all Glypican-3 products
ApplicationsICC/IF, WB
Application Note
Suggested dilutionReference
Immunocytochemistry/ Immunofluorescence (ICC/IF)1:100-1:1000*  
Western Blot (WB)1:500-1:3000*  
Not tested in other applications.
*Optimal dilutions/concentrations should be determined by the researcher.
Predicted Target Size66 kDa (note) IsoForm (4)
Positive ControlsHepG2 , HepG2 (24 μg/ml Tunicamycin treatment for 16 hr)
Form SuppliedLiquid
Concentration0.27 mg/ml (Please refer to the vial label for the specific concentration)
PurificationPurified by antigen-affinity chromatography.

  • Specifications: Glypican-3 antibody

Full Nameglypican 3
BackgroundCell surface heparan sulfate proteoglycans are composed of a membrane-associated protein core substituted with a variable number of heparan sulfate chains. Members of the glypican-related integral membrane proteoglycan family (GRIPS) contain a core protein anchored to the cytoplasmic membrane via a glycosyl phosphatidylinositol linkage. These proteins may play a role in the control of cell division and growth regulation. Deletion mutations in this gene are associated with Simpson-Golabi-Behmel syndrome. [provided by RefSeq]
SynonymsDGSX antibody , GPC3 antibody , GPC3 antibody , GTR22 antibody , MXR7 antibody , OCI5 antibody , SDYS antibody , SGB antibody , SGBS antibody , SGBS1 antibody , glypican 3 antibody
Cellular LocalizationCell membrane; Lipid-anchor , GPI-anchor; Extracellular side , Secreted glypican-3: Secreted , extracellular space
HostRabbit
ClonalityPolyclonal
IsotypeIgG
ImmunogenRecombinant protein encompassing a sequence within the C-terminus region of human GPC3. The exact sequence is proprietary.
Antigen SpeciesHuman
Species ReactivityHuman
Predicted Cross Reactivity species Predicted cross-reactivity:
    Predicted cross-reactivity is based on sequence homology, with greater than 80% immunogen sequence identity considered positive.
    Please note that we are only able to guarantee products to work in applications and species in which they have been tested.
Mouse, Rat, Chimpanzee, Bovine
Predict Reactivity NoteMouse (81%), Rat (80%), Chimpanzee (96%), Bovine (85%)
ConjugationUnconjugated

  • Storage Conditions: Glypican-3 antibody

Storage Buffer1XPBS, 1% BSA, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.
Storage InstructionKeep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
NotesFor In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
ResearchArea
  • Cancer > Tumor biomarkers
  • Stem Cell Development > Development > Morphogenesis
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Glypican-3 antibody validated data
  • Anti-Glypican-3 antibody used in Western Blot (WB). GTX130732
    • GTX130732 WB Image  Biological & Orthogonal
      Untreated (–) and treated (+) HepG2 whole cell extracts (30 µg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Glypican-3 antibody (GTX130732) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
      Top
  • Anti-Glypican-3 antibody used in Western Blot (WB). GTX130732
    • GTX130732 WB Image
      Whole cell extract (30 µg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with GPC3 antibody (GTX130732) diluted at 1:1000.
      Top
  • Anti-Glypican-3 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX130732
    • GTX130732 ICC/IF Image
      Glypican-3 antibody detects Glypican-3 protein at cell membrane by immunofluorescent analysis.
      Sample: HepG2 cells were fixed in 4% paraformaldehyde at RT for 15 min.
      Green: Glypican-3 protein stained by Glypican-3 antibody (GTX130732) diluted at 1:400.
      Blue: Hoechst 33342 staining.
      Top
  • Anti-Glypican-3 antibody used in Western Blot (WB). GTX130732
    • GTX130732 WB Image  Biological & Orthogonal
      Untreated (–) and treated (+) HepG2 whole cell extracts (30 µg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Glypican-3 antibody (GTX130732) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
      Top
  • Anti-Glypican-3 antibody used in Western Blot (WB). GTX130732
    • GTX130732 WB Image
      Whole cell extract (30 µg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with GPC3 antibody (GTX130732) diluted at 1:1000.
      Top
  • Anti-Glypican-3 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX130732
    • GTX130732 ICC/IF Image
      Glypican-3 antibody detects Glypican-3 protein at cell membrane by immunofluorescent analysis.
      Sample: HepG2 cells were fixed in 4% paraformaldehyde at RT for 15 min.
      Green: Glypican-3 protein stained by Glypican-3 antibody (GTX130732) diluted at 1:400.
      Blue: Hoechst 33342 staining.
      Top
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Target Bioinfo/ Predicted Isoform Cross Reactivity
SpeciesGene nameGene IDSwissProtOMIMIsoform (mRNA)Protein Accession#MW
Homo sapiensGPC32719P51654194070, 300037, 312870NM_001164617NP_00115808968.43
Homo sapiensGPC32719P51654194070, 300037, 312870NM_001164618NP_00115809063.74
Homo sapiensGPC32719P51654194070, 300037, 312870NM_001164619NP_00115809159.49
Homo sapiensGPC32719P51654194070, 300037, 312870NM_004484NP_00447565.58

Cross-react Bioinfo
SpeciesGene nameGene IDSwissProtIsoform (mRNA)Protein Accession#MW
Rattus norvegicusGpc325236P13265NM_012774NP_03690667.06
Mus musculusGpc314734Q3TWB2, Q8CFZ4NM_016697NP_05790665.34
    *MSDS
    MSDS-Glycerol.pdf
    MSDS-PBS.pdf
    MSDS-ProClin.pdf
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