Granzyme B antibody [GB11]
|Applications||ELISA, FACS, ICC/IF, IHC-Fr, IP|
| Immunocytochemistry/ Immunofluorescence||Assay-dependent dilution
| IHC (Frozen sections)||Assay-dependent dilution
| Immunoprecipitation||Assay-dependent dilution
Flow cytometry/FACS : Use 10μl of the suggested working dilution to label 106 cells or 100μl whole blood.Not tested in other applications.
Immunocytochemistry/ Immunofluorescence : Membrane permeabilisation is required for this application.
*Optimal dilutions/concentrations should be determined by the researcher.
(Please refer to the vial label for the specific concentration)|
|Purification||Protein A purified|
|Purification Note||From tissue culture supernatant|
|Full Name||granzyme B|
|Product Description||Mouse monoclonal antibody [GB11] to Granzyme B|
|Background||Cytolytic T lymphocytes (CTL) and natural killer (NK) cells share the remarkable ability to recognize, bind, and lyse specific target cells. They are thought to protect their host by lysing cells bearing on their surface 'nonself' antigens, usually peptides or proteins resulting from infection by intracellular pathogens. The protein encoded by this gene is crucial for the rapid induction of target cell apoptosis by CTL in cell-mediated immune response. [provided by RefSeq, Jul 2008]|
|Synonyms||granzyme B (granzyme 2, cytotoxic T-lymphocyte-associated serine esterase 1) Antibody , CCPI Antibody , CGL1 Antibody , GZMB Antibody , CTSGL1 Antibody , CGL-1 Antibody , CSP-B Antibody , CTLA1 Antibody , CSPB Antibody , SECT Antibody , HLP Antibody|
|Specificity||This antibody has been reported to function as a capture reagent in sandwich ELISA assays for soluble Granzyme B in conjunction with biotin conjugated GB10 (GTX75842) as detection reagent.|
|Immunogen||Purified human Granzyme B.|
|Species Reactivity||Human, Monkey, Chimpanzee|