|Applications||ICC/IF, IHC-P, WB|
Not tested in other applications.
|IHC (Formalin-fixed paraffin-embedded sections)||1:100-1:1000*
*Optimal dilutions/concentrations should be determined by the researcher.
|Predicted Target Size||72 kDa (note)
|Positive Controls||293T , A431 , HeLa , HepG2 , NIH-3T3 , JC , BCL-1 , PC-12 , Rat2|
(Please refer to the vial label for the specific concentration)|
|Purification||Purified by antigen-affinity chromatography.|
|Full Name||heat shock 70kDa protein 5 (glucose-regulated protein, 78kDa)|
|Product Description||Rabbit Polyclonal antibody to Grp78 (78 kDa glucose-regulated protein)|
|Background||When Chinese hamster K12 cells are starved of glucose, the synthesis of several proteins, called glucose-regulated proteins (GRPs), is markedly increased. Hendershot et al. (1994) [PubMed 8020977] pointed out that one of these, GRP78 (HSPA5), also referred to as 'immunoglobulin heavy chain-binding protein' (BiP), is a member of the heat-shock protein-70 (HSP70) family and is involved in the folding and assembly of proteins in the endoplasmic reticulum (ER). Because so many ER proteins interact transiently with GRP78, it may play a key role in monitoring protein transport through the cell.[supplied by OMIM]|
|Synonyms||BIP antibody, FLJ26106 antibody, GRP78 antibody, MIF2 antibody, HSPA5 antibody, immunoglobulin heavy chain-binding protein antibody, 78 kDa glucose-regulated protein antibody, endoplasmic reticulum lumenal Ca(2+)-binding protein grp78 antibody, "heat shock 70kDa protein 5 (glucose-regulated protein, 78kDa) antibody"|
|Cellular Localization||Endoplasmic reticulum lumen , Melanosome |
|Immunogen||Recombinant protein encompassing a sequence within the center region of human Grp78. The exact sequence is proprietary.|
|Species Reactivity||Human, Mouse, Rat|
|Predicted Cross Reactivity species Predicted cross-reactivity:|
Predicted cross-reactivity is based on sequence homology, with greater than 80% immunogen sequence identity considered positive.
Please note that we are only able to guarantee products to work in applications and species in which they have been tested.
|Zebrafish, Japanese medaka, Xenopus laevis, Chicken, Rhesus Monkey, Bovine|
|Predict Reactivity Note||Zebrafish (95%), Japanese medaka (95%), Xenopus laevis (99%), Chicken (99%), Rhesus Monkey (100%), Bovine (100%)|