|Applications||ICC/IF, IHC-P, WB|
Not tested in other applications.
|IHC (Formalin-fixed paraffin-embedded sections)||1:100-1:1000*
*Optimal dilutions/concentrations should be determined by the researcher.
|Predicted Target Size||21 kDa (note)
|Positive Controls||293T , A431 , HeLa , HepG2 , Molt-4 , 293T transfected with H-Ras , PC-12 , Rat2 , *RFL6 , *PLC5Rat2 , *RFL6 , *PLC5|
(Please refer to the vial label for the specific concentration)|
|Purification||Purified by antigen-affinity chromatography.|
|Full Name||v-Ha-ras Harvey rat sarcoma viral oncogene homolog|
|Product Description||Rabbit Polyclonal antibody to H-Ras (v-Ha-ras Harvey rat sarcoma viral oncogene homolog)|
|Background||This gene belongs to the Ras oncogene family, whose members are related to the transforming genes of mammalian sarcoma retroviruses. The products encoded by these genes function in signal transduction pathways. These proteins can bind GTP and GDP, and they have intrinsic GTPase activity. This protein undergoes a continuous cycle of de- and re-palmitoylation, which regulates its rapid exchange between the plasma membrane and the Golgi apparatus. Mutations in this gene cause Costello syndrome, a disease characterized by increased growth at the prenatal stage, growth deficiency at the postnatal stage, predisposition to tumor formation, mental retardation, skin and musculoskeletal abnormalities, distinctive facial appearance and cardiovascular abnormalities. Defects in this gene are implicated in a variety of cancers, including bladder cancer, follicular thyroid cancer, and oral squamous cell carcinoma. Multiple transcript variants, which encode different isoforms, have been identified for this gene. [provided by RefSeq]|
|Synonyms||C-BAS/HAS antibody, C-H-RAS antibody, C-HA-RAS1 antibody, CTLO antibody, H-RASIDX antibody, HAMSV antibody, HRAS1 antibody, K-RAS antibody, N-RAS antibody, RASH1 antibody, HRAS antibody, transforming protein p21 antibody, Ha-Ras1 proto-oncoprotein antibody, H-Ras-1 antibody, GTPase HRas antibody, c-has/bas p21 protein antibody, transformation gene: oncogene HAMSV antibody, p19 H-RasIDX protein antibody, GTP- and GDP-binding peptide B antibody, p21ras antibody, Ras family small GTP binding protein H-Ras antibody, ha-Ras antibody, c-ras-Ki-2 activated oncogene antibody, v-Ha-ras Harvey rat sarcoma viral oncogene homolog antibody|
|Cellular Localization||Cell membrane; Lipid-anchor; Cytoplasmic side , Golgi apparatus membrane; Lipid-anchor |
|Specificity||This antibody is specific for H-Ras protein, and it does not cross react with human N-Ras or K-Ras protein.|
|Immunogen||Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human H-Ras. The exact sequence is proprietary.|
|Species Reactivity||Human, Mouse, Rat|
|Predicted Cross Reactivity species Predicted cross-reactivity:|
Predicted cross-reactivity is based on sequence homology, with greater than 80% immunogen sequence identity considered positive.
Please note that we are only able to guarantee products to work in applications and species in which they have been tested.
|Xenopus laevis, Chicken, Rhesus Monkey|
|Predict Reactivity Note||Xenopus laevis (100%), Chicken (100%), Rhesus Monkey (100%)|