|Applications||ICC/IF, IHC-P, IP, WB, ChIP assay|
Not tested in other applications.
| ChIP assay||Assay-dependent dilution
|IHC (Formalin-fixed paraffin-embedded sections)||1:100-1:1000*
*Optimal dilutions/concentrations should be determined by the researcher.
|Predicted Target Size||55 kDa (note)
|Positive Controls||293T , A431 , HeLa , HepG2 , NIH-3T3 , U87-MG , SK-N-SH , *E8.5 embryo|
(Please refer to the vial label for the specific concentration)|
|Purification||Purified by antigen-affinity chromatography.|
|Full Name||histone deacetylase 1|
|Product Description||Rabbit Polyclonal antibody to HDAC1 (histone deacetylase 1)|
|Background||Histone acetylation and deacetylation, catalyzed by multisubunit complexes, play a key role in the regulation of eukaryotic gene expression. The protein encoded by this gene belongs to the histone deacetylase/acuc/apha family and is a component of the histone deacetylase complex. It also interacts with retinoblastoma tumor-suppressor protein and this complex is a key element in the control of cell proliferation and differentiation. Together with metastasis-associated protein-2, it deacetylates p53 and modulates its effect on cell growth and apoptosis. [provided by RefSeq]|
|Synonyms||DKFZp686H12203 antibody, GON-10 antibody, HD1 antibody, RPD3 antibody, RPD3L1 antibody, HDAC1 antibody, histone deacetylase 1 antibody, reduced potassium dependency, yeast homolog-like 1 antibody|
|Immunogen||Recombinant protein encompassing a sequence within the center region of human HDAC1. The exact sequence is proprietary.|
|Species Reactivity||Human, Mouse, Rat|
|Predicted Cross Reactivity species Predicted cross-reactivity:|
Predicted cross-reactivity is based on sequence homology, with greater than 80% immunogen sequence identity considered positive.
Please note that we are only able to guarantee products to work in applications and species in which they have been tested.
|Predict Reactivity Note||Bovine (98%)|