|Applications||ICC/IF, IHC-P, IP, WB, ChIP assay|
| ChIP assay||Assay-dependent dilution
|IHC (Formalin-fixed paraffin-embedded sections)||1:100-1:1000*
IHC (Formalin-fixed paraffin-embedded sections) : suggested antigen retrieval using heat mediated 10mM Citrate buffer (pH6.0) or Tris-EDTA buffer (pH8.0)Not tested in other applications.
*Optimal dilutions/concentrations should be determined by the researcher.
|Predicted Target Size||55 kDa (note)
|Positive Controls||293T , A431 , HeLa , HepG2 , C2C12 , Rat2 , *NB2a|
|Purification||Purified by antigen-affinity chromatography.|
|Full Name||histone deacetylase 2|
|Product Description||Rabbit Polyclonal antibody to HDAC2 (histone deacetylase 2)|
|Background||This gene product belongs to the histone deacetylase family. Histone deacetylases act via the formation of large multiprotein complexes and are responsible for the deacetylation of lysine residues on the N-terminal region of the core histones (H2A, H2B, H3 and H4). This protein also forms transcriptional repressor complexes by associating with many different proteins, including YY1, a mammalian zinc-finger transcription factor. Thus it plays an important role in transcriptional regulation, cell cycle progression and developmental events. [provided by RefSeq]|
|Synonyms||HD2 antibody, RPD3 antibody, YAF1 antibody, HDAC2 antibody, histone deacetylase 2 antibody, transcriptional regulator homolog RPD3 antibody, YY1-associated factor 1 antibody|
|Cellular Localization||Nucleus |
|Immunogen||Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human HDAC2. The exact sequence is proprietary.|
|Species Reactivity||Human, Mouse, Rat|