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HDAC3 antibody [7G6C5]

Anti-HDAC3 antibody [7G6C5] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX83173
Anti-HDAC3 antibody [7G6C5] used in Western Blot (WB). GTX83173

Cat. No. GTX83173

Reference 1

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 7G6C5

Isotype

 IgG2a

Application

 WB, ICC/IF, ELISA

Reactivity

 Human, Mouse, Rat
Package
100 μl ($409)
See all HDAC3 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1/500 - 1/2000
ICC/IF 1/200 - 1/1000
ELISA 1/10000
Not tested in other applications.

Calculated MW

49 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

Ascites

Preservative

0.03% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Antigen Species

Human

Immunogen

Purified recombinant fragment of human HDAC3 (aa224-428) expressed in E. Coli.

Purification

Unpurified

Conjugation

Unconjugated

RRID

AB_11165075

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

histone deacetylase 3 , HD3 , KDAC3 , RPD3 , RPD3-2

Cellular Localization

Nucleus,Cytoplasm,Cytoplasm, cytosol

Background

Histones play a critical role in transcriptional regulation, cell cycle progression, and developmental events. Histone acetylation/deacetylation alters chromosome structure and affects transcription factor access to DNA. The protein encoded by this gene belongs to the histone deacetylase/acuc/apha family. It has histone deacetylase activity and represses transcription when tethered to a promoter. It may participate in the regulation of transcription through its binding with the zinc-finger transcription factor YY1. This protein can also down-regulate p53 function and thus modulate cell growth and apoptosis. This gene is regarded as a potential tumor suppressor gene. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-HDAC3 antibody [7G6C5] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX83173

GTX83173 ICC/IF Image

ICC/IF analysis of HeLa cells using GTX83173 HDAC3 antibody [7G6C5].
Green : HDAC3
Red: Actin filaments

Anti-HDAC3 antibody [7G6C5] used in Western Blot (WB). GTX83173

GTX83173 WB Image

WB analysis of HeLa (1), NIH3T3 (2), C6 (3) and COS (4) cell lysate using GTX83173 HDAC3 antibody [7G6C5].

REFERENCE

Application Reference

Simbriger K et al. J Neurosci Methods 2019; : 108456 Monitoring translation in synaptic fractions using a ribosome profiling strategy.
Application : WB
Reactivity : Mouse
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REVIEW

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SDS
Sodium Azide.pdf
Package List Price ($)
$ 409