|Applications||ICC/IF, IHC-P, IP, WB, ChIP assay|
Not tested in other applications.
| ChIP assay||Assay-dependent dilution
|IHC (Formalin-fixed paraffin-embedded sections)||1:100-1:1000*
*Optimal dilutions/concentrations should be determined by the researcher.
|Predicted Target Size||49 kDa (note)
|Positive Controls||293T , A431 , HeLa , HepG2 , U87-MG , SK-N-SH , IMR32 , SK-N-AS , *NB2a|
(Please refer to the vial label for the specific concentration)|
|Purification||Purified by antigen-affinity chromatography.|
|Full Name||histone deacetylase 3|
|Product Description||Rabbit Polyclonal antibody to HDAC3 (histone deacetylase 3)|
|Background||Histones play a critical role in transcriptional regulation, cell cycle progression, and developmental events. Histone acetylation/deacetylation alters chromosome structure and affects transcription factor access to DNA. The protein encoded by this gene belongs to the histone deacetylase/acuc/apha family. It has histone deacetylase activity and represses transcription when tethered to a promoter. It may participate in the regulation of transcription through its binding with the zinc-finger transcription factor YY1. This protein can also down-regulate p53 function and thus modulate cell growth and apoptosis. This gene is regarded as a potential tumor suppressor gene. [provided by RefSeq]|
|Synonyms||HD3 antibody, RPD3 antibody, RPD3-2 antibody, HDAC3 antibody, SMAP45 antibody, histone deacetylase 3 antibody|
|Immunogen||Recombinant protein encompassing a sequence within the center region of human HDAC3. The exact sequence is proprietary.|
|Species Reactivity||Human, Mouse, Rat|
|Predicted Cross Reactivity species Predicted cross-reactivity:|
Predicted cross-reactivity is based on sequence homology, with greater than 80% immunogen sequence identity considered positive.
Please note that we are only able to guarantee products to work in applications and species in which they have been tested.
|Zebrafish, Pig, Chicken, Rhesus Monkey, Bovine|
|Predict Reactivity Note||Zebrafish (89%), Pig (100%), Chicken (95%), Rhesus Monkey (100%), Bovine (100%)|