Find Antibodies, Antibody Supplier - GeneTex Inc.
United States
  • Please select
    • All categories
    • Primary antibodies
    • Secondary antibodies
    • Antibody panels
    • Small molecules
    • Proteins
    • Peptides
    • Lysates
    • Slides
    • Sera & plasma
    • Research reagents
    • Research kits
    • Assay kits
    • ELISA kits
    • ELISA pairs
    • Isotype controls
My Account My Cart
Please login Product Support| Company News| Contact Us| SiteMap
  • Primary Antibodies

  • Secondary Antibodies

  • Proteins & Peptides

  • Lysates & Slides

  • Serum & Reagents

  • Research Kits

  • Isotype Controls

  • Home
  • Primary Antibodies
  • Primary antibodies
  • HELIOS antibody [N3C3]

Related products
More Hide
Recently viewed products

1 .GTX115629 HELIOS antibody [N3C3]

GTX101435 beta Catenin antibody [N1N2-2], N-term
Anti-beta Catenin antibody [N1N2-2], N-term used in IHC-P. GTX101435
GTX122148 Histone H3 antibody
Anti-Histone H3 antibody used in IHC-P. GTX122148
GTX128508 mCherry antibody
Anti-mCherry antibody used in IHC-Wm. GTX128508
GTX629448 5-Methylcytosine / 5-mC antibody [GT4111]
Anti-5-Methylcytosine / 5-mC antibody [GT4111] used in Dot. GTX629448
GTX629765 5-Hydroxymethylcytosine / 5-hmC antibody [GT13612]
Anti-5-Hydroxymethylcytosine / 5-hmC antibody [GT13612] used in MeDIP. GTX629765
GTX629890 SQSTM1 / P62 antibody [GT1478]
Anti-SQSTM1 / P62 antibody [GT1478] used in WB. GTX629890
ECL kit RIPA Extraction Kit Protein Ladder

HELIOS antibody [N3C3]

Biological & Orthogonal

  • Host / ClonalityRabbit Polyclonal
  • ApplicationsIHC, IHC-P, WB
  • Species reactivityHuman
  • Publication  ( 2 )
Cat No: GTX115629
Package Price Qty
25 μlUSD 119.00
100 μlUSD 319.00
Add to cart
 
  • Anti-HELIOS antibody [N3C3] used in IHC (Formalin-fixed paraffin-embedded sections). GTX115629
  • Anti-HELIOS antibody [N3C3] used in Western Blot (WB). GTX115629
LinkButton
  • Datasheet
  • Star Review (0)
    Reference (2)
  • Bio-info
  • Protocol & MSDS
  • Application Information: HELIOS antibody [N3C3]

Catalog Number GTX115629
☆☆☆☆☆
( 0 )
Publication ( 2 )
Product Name HELIOS antibody [N3C3]
See all HELIOS products
ApplicationsIHC, IHC-P, WB
Application Note
Suggested dilutionReference
Immunohistochemistry (IHC)Assay-dependent dilution Y
IHC (Formalin-fixed paraffin-embedded sections)1:100-1:1000*  
Western Blot (WB)1:500-1:3000*  
Not tested in other applications.
*Optimal dilutions/concentrations should be determined by the researcher.
Predicted Target Size60 kDa (note) IsoForm (3)
Positive ControlsJurkat , Jurkat NE
Form SuppliedLiquid
Concentration1 mg/ml (Please refer to the vial label for the specific concentration)
PurificationPurified by antigen-affinity chromatography.

  • Specifications: HELIOS antibody [N3C3]

Full NameIKAROS family zinc finger 2 (Helios)
BackgroundThis gene encodes a member of the Ikaros family of zinc-finger proteins. Three members of this protein family (Ikaros, Aiolos and Helios) are hematopoietic-specific transcription factors involved in the regulation of lymphocyte development. This protein forms homo- or hetero-dimers with other Ikaros family members, and is thought to function predominantly in early hematopoietic development. Multiple transcript variants encoding different isoforms have been found for this gene, but the biological validity of some variants has not been determined. [provided by RefSeq]
SynonymsHELIOS antibody, MGC34330 antibody, ZNF1A2 antibody, ZNFN1A2 antibody, IKZF2 antibody, zinc finger DNA binding protein Helios antibody, ikaros family zinc finger protein 2 antibody, "zinc finger protein, subfamily 1A, 2 (Helios) antibody", zinc finger protein Helios antibody, IKAROS family zinc finger 2 (Helios) antibody
Cellular LocalizationNucleus
HostRabbit
ClonalityPolyclonal
IsotypeIgG
ImmunogenRecombinant protein encompassing a sequence within the center region of human HELIOS. The exact sequence is proprietary.
Antigen SpeciesHuman
Species ReactivityHuman
Predicted Cross Reactivity species Predicted cross-reactivity:
    Predicted cross-reactivity is based on sequence homology, with greater than 80% immunogen sequence identity considered positive.
    Please note that we are only able to guarantee products to work in applications and species in which they have been tested.
Mouse, Chicken, Rhesus Monkey
Predict Reactivity NoteMouse (96%), Chicken (87%), Rhesus Monkey (99%)
ConjugationUnconjugated

  • Storage Conditions: HELIOS antibody [N3C3]

Storage Buffer0.1M Tris, 0.1M Glycine, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.
Storage InstructionKeep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
NotesFor In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
HELIOS antibody [N3C3] validated data
  • Anti-HELIOS antibody [N3C3] used in IHC (Formalin-fixed paraffin-embedded sections). GTX115629
    • GTX115629 IHC-P Image
      Immunohistochemical analysis of paraffin-embedded human ovarian cancer, using HELIOS(GTX115629) antibody at 1:250 dilution.
      Top
  • Anti-HELIOS antibody [N3C3] used in Western Blot (WB). GTX115629
    • GTX115629 WB Image
      HELIOS antibody detects HELIOS protein by western blot analysis. Jurkat whole cell extracts and nuclear extracts (30 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with HELIOS antibody (GTX115629) diluted by 1:500 and developed with Trident femto Western HRP Substrate (GTX14698). The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
      Top
  • Anti-HELIOS antibody [N3C3] used in IHC (Formalin-fixed paraffin-embedded sections). GTX115629
    • GTX115629 IHC-P Image
      Immunohistochemical analysis of paraffin-embedded human ovarian cancer, using HELIOS(GTX115629) antibody at 1:250 dilution.
      Top
  • Anti-HELIOS antibody [N3C3] used in Western Blot (WB). GTX115629
    • GTX115629 WB Image
      HELIOS antibody detects HELIOS protein by western blot analysis. Jurkat whole cell extracts and nuclear extracts (30 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with HELIOS antibody (GTX115629) diluted by 1:500 and developed with Trident femto Western HRP Substrate (GTX14698). The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
      Top
Back to Top
  • Reference for HELIOS antibody [N3C3]
Application References Application Reactivity
Amodio D et al. Quantitative Multiplexed Imaging Analysis Reveals a Strong Association between Immunogen-Specific B Cell Responses and Tonsillar Germinal Center Immune Dynamics in Children after Influenza Vaccination. J Immunol (Epub ahead of print) (2017). IHC Human
Muto S et al. Clinical significance of expanded Foxp3+ Helios- regulatory T cells in patients with non-small cell lung cancer. Int J Oncol 47 (6):2082-90 (2015).
Target Bioinfo/ Predicted Isoform Cross Reactivity
SpeciesGene nameGene IDSwissProtOMIMIsoform (mRNA)Protein Accession#MW
Homo sapiensIKZF222807Q9UKS7606234BC028936AAH2893656.77
Homo sapiensIKZF222807Q9UKS7606234NM_001079526NP_00107299456.77
Homo sapiensIKZF222807Q9UKS7606234NM_016260NP_05734459.58

Cross-react Bioinfo
SpeciesGene nameGene IDSwissProtIsoform (mRNA)Protein Accession#MW
Mus musculusIkzf222779Q8C8A3NM_011770NP_03590059.41
    *MSDS
    MSDS-Glycerol.pdf
    MSDS-Thimerosal.pdf
  • Product types

    Primary Antibodies Secondary Antibodies Proteins & Peptides Lysates & Slides Serum & Reagents Research Kits Isotype Controls

    Resources

    Literature Newsletters Protocols Pathways

  • Research Areas

    Cancer Cell Biology Neuroscience Metabolism Epigenetics Immunology Signal Transduction Infectious Disease Disease Related Stem Cell Development Zebrafish Epitope Tags

  • Company

    About Us News Rewards Program Recent Publications Contact Us Policy Guarantee Careers Sitemap

    Support

    Protocols Technical Tips FAQ

  • Distributors

    Custom Antibodies

    Member Account

    • Youtube Youtube
    • Facebook Facebook
    • Twitter Twitter
    • LinkedIn LinkedIn
    • Weibo Weibo
© 2013 GeneTex Inc. All rights reserved.
All products are for research use only—Not for use in diagnostic procedures.