HIF1 alpha antibody
|Applications||ICC/IF, IHC-Fr, IHC-P, IP, WB, ChIP assay|
Not tested in other applications.
| ChIP assay||Assay-dependent dilution
| IHC (Frozen sections)||Assay-dependent dilution
|IHC (Formalin-fixed paraffin-embedded sections)||1:100-1:1000*
*Optimal dilutions/concentrations should be determined by the researcher.
|Predicted Target Size||93 kDa (note)
|Positive Controls||HeLa , HeLa (500 μM CoCl2 treatment for 24 hr) , HepG2 whole cell lysate/extract (1% O2 treatment for 24hr) , NIH-3T3 (500 μM CoCl2 treatment for 24 hr) , 293T transfected HIF1A , HepG2 ( 200 μM CoCl2 treatment for 24 hr) , HepG2 (500 μM CoCl2 treatment for 24 hr)|
(Please refer to the vial label for the specific concentration)|
|Purification||Purified by antigen-affinity chromatography.|
|Full Name||hypoxia inducible factor 1, alpha subunit (basic helix-loop-helix transcription factor)|
|Product Description||Rabbit Polyclonal antibody to HIF1 alpha (hypoxia inducible factor 1, alpha subunit (basic helix-loop-helix transcription factor))|
|Background||Hypoxia-inducible factor-1 (HIF1) is a transcription factor found in mammalian cells cultured under reduced oxygen tension that plays an essential role in cellular and systemic homeostatic responses to hypoxia. HIF1 is a heterodimer composed of an alpha subunit and a beta subunit. The beta subunit has been identified as the aryl hydrocarbon receptor nuclear translocator (ARNT). This gene encodes the alpha subunit of HIF-1. Overexpression of a natural antisense transcript (aHIF) of this gene has been shown to be associated with nonpapillary renal carcinomas. Two alternative transcripts encoding different isoforms have been identified. [provided by RefSeq]|
|Synonyms||HIF-1alpha antibody, HIF1 antibody, HIF1-ALPHA antibody, MOP1 antibody, PASD8 antibody, bHLHe78 antibody, HIF1A antibody, basic-helix-loop-helix-PAS protein MOP1 antibody, class E basic helix-loop-helix protein 78 antibody, ARNT-interacting protein antibody, member of PAS superfamily 1 antibody, hypoxia-inducible factor 1, alpha subunit (basic helix-loop-helix transcription factor) antibody, hypoxia-inducible factor 1-alpha antibody, hypoxia-inducible factor 1 alpha isoform I.3 antibody, PAS domain-containing protein 8 antibody, member of PAS protein 1 antibody, HIF-1-alpha antibody, ARNT interacting protein antibody, hypoxia inducible factor 1, alpha subunit (basic helix-loop-helix transcription factor) antibody|
|Cellular Localization||Cytoplasm , Nucleus |
|Specificity||Knockdown/Knockout validation was supported by references (PMID:25428606)|
|Immunogen||Recombinant protein encompassing a sequence within the C-terminus region of human HIF1 alpha. The exact sequence is proprietary.|
|Species Reactivity||Human, Mouse, Rat, Bovine|
|Predicted Cross Reactivity species Predicted cross-reactivity:|
Predicted cross-reactivity is based on sequence homology, with greater than 80% immunogen sequence identity considered positive.
Please note that we are only able to guarantee products to work in applications and species in which they have been tested.
|Dog, Pig, Rabbit, Bovine|
|Predict Reactivity Note||Dog (97%), Pig (95%), Rabbit (95%), Bovine (97%)|