HIF1 alpha antibody [H1alpha67]
|Applications||ELISA, FACS, GSA, ICC/IF, IHC, IHC-Fr, IHC-P, IP, WB, ChIP assay|
|Application Note||Recommended Starting Dilutions:|
For WB: Use at a dilution of 1:500.
For ChIP: Use at a dilution of 1:10-1:500.
For Gel Super Shift Assays: Use at a dilution of 1:1-1:100
For ICC/IF:Use at a dilution of 1:50.
For IHC:Use at a dilution of 1:20-1:50.
For IHC-Fr: Use at a dilution of 1:20-1:50.
For IHC-P: Use at a dilution of 1:20-1:50
For IP: Use at a dilution of 1:10-1:500. Optimal dilutions/cancentrations should be determined by researcher.
|Positive Controls||Nuclear extracts recommended|
|Purification||Protein G purified|
|Full Name||hypoxia inducible factor 1, alpha subunit (basic helix-loop-helix transcription factor)|
|Product Description||Mouse monoclonal [H1alpha67] to Hypoxia Inducible Factor-1 alpha|
|Background||Hypoxia contributes significantly to the pathophysiology of major categories of human disease, including myocardial and cerebral ischemia, cancer, pulmonary hypertension, congenital heart disease and chronic obstructive pulmonary disease. HIF-1 is a nuclear protein that activates gene transcription in response to reduced cellular 02 concentration. HIF-1 activates the transcription of EPO, VEGF, iNOS, heme oxygenase 1 and several other critical intracellular responses to hypoxia. HIF-1 is a heterodimer composed of HIF-1a and HIF-1b subunits. Both subunits are induced by hypoxia and rapidly decay upon return to normoxia and are basic-helix-loop-helix-PAS proteins. Recent research indicates the ability to regulate hypoxia-inducible factors may be related to tumor-related angiogenesis in certain cancers.|
|Synonyms||HIF1 alpha, HIF-1alpha, HIF1A, MOP1, PASD8, HIF1-ALPHA, 603348, HIF1ALPHA, Q16665, 3091, HIF 1alpha|
|Cellular Localization||HIF-1 is a nuclear protein. |
|Specificity||This antibody is specific for HIF-1 alpha.|
|Immunogen||Amino acids 432-528 of human HIF-1alpha.|
|Species Reactivity||Human, Mouse, Pig, Rat, Yeast, Ferret, Primate, Rabbit, Sheep, Bovine|