APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 1:500-1:3000 |
| 1:100-1:1000 |
| 1:100-1:1000 |
Not tested in other applications.
Calculated MW
Positive Control
293T , A431 , HeLa , HepG2 , HepG2 ( 1% O2 treatment for 24 hr)
Predict Reactivity
Rat, Rabbit, Sheep, Bovine, Guinea pig, Pig(>80% identity)
PROPERTIES
Form
Liquid
Buffer
PBS, 20% Glycerol
Preservative
0.01% Thimerosal
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
1 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Recombinant protein encompassing a sequence within the C-terminus region of human HIF1 beta. The exact sequence is proprietary.
Purification
Purified by antigen-affinity chromatography.
Conjugation
Unconjugated
RRID
AB_2861418
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
aryl hydrocarbon receptor nuclear translocator , HIF-1-beta , HIF-1beta , HIF1-beta , HIF1B , HIF1BETA , TANGO , bHLHe2
Cellular Localization
Nucleus
Background
The aryl hydrocarbon (Ah) receptor is involved in the induction of several enzymes that participate in xenobiotic metabolism. The ligand-free, cytosolic form of the Ah receptor is complexed to heat shock protein 90. Binding of ligand, which includes dioxin and polycyclic aromatic hydrocarbons, results in translocation of the ligand-binding subunit only to the nucleus. Induction of enzymes involved in xenobiotic metabolism occurs through binding of the ligand-bound Ah receptor to xenobiotic responsive elements in the promoters of genes for these enzymes. This gene encodes a protein that forms a complex with the ligand-bound Ah receptor, and is required for receptor function. The encoded protein has also been identified as the beta subunit of a heterodimeric transcription factor, hypoxia-inducible factor 1 (HIF1). A t(1;12)(q21;p13) translocation, which results in a TEL-ARNT fusion protein, is associated with acute myeloblastic leukemia. Three alternatively spliced variants encoding different isoforms have been described for this gene. [provided by RefSeq]
Database
Research Area
DATA IMAGES
|
GTX128795 IHC-P Image
HIF1 beta antibody detects HIF1 beta protein at nucleus on mouse esophagus by immunohistochemical analysis.
Sample: Paraffin-embedded mouse esophagus.
HIF1 beta antibody (GTX128795) dilution: 1:500.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min
|
|
|
GTX128795 WB Image
HIF1 beta antibody detects HIF1 beta protein by western blot analysis.
A. 30 μg HepG2 whole cell lysate/extract (untreated)
B. 30 μg HepG2 whole cell lysate/extract ( 1% O2 treatment for 24 hr)
7.5 % SDS-PAGE
HIF1 beta antibody (GTX128795) dilution: 1:1000
|
|
|
GTX128795 WB Image
HIF1 beta antibody detects HIF1 beta protein by western blot analysis.
A. 30 μg 293T whole cell lysate/extract
B. 30 μg A431whole cell lysate/extract
C. 30 μg HeLa whole cell lysate/extract
7.5 % SDS-PAGE
HIF1 beta antibody (GTX128795) dilution: 1:1000
|
|
|
GTX128795 ICC/IF Image
HIF1 beta antibody detects HIF1 beta protein at nucleus by immunofluorescent analysis.
Samples: HeLa cells mock (left) and treated with 500μM CoCl2 for 24hr (right) were fixed in 4% paraformaldehyde at RT for 15 min.
Green: HIF1 beta protein stained by HIF1 beta antibody (GTX128795) diluted at 1:500.
Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:1000.
|
REFERENCE
There are currently no references for HIF1 beta antibody (GTX128795). Be the first to share your publications with this product.
REVIEW
HIF1 beta antibody
Cat. No. GTX128795
|
Rating
|
|
( Average 4 based on 2 users reviews)
|
Application
|
Western Blot(WB)
|
|
( Average 4 based on 2 users reviews)
|
Date :
Anonymous submitted on 08-Sep-2015
Application Tested :
WB
Sample Species :
Ms
Sample :
skin fibroblast tissue
Amount used :
5μg
Blocking :
1% skim milk in TBST, 25°C, 1Hr
Primary Antibody :
1:1000, 4°C, 16Hr
Date :
Anonymous submitted on 19-Aug-2015
Application Tested :
WB
Sample Species :
Ms
Sample :
B16 melanoma cells
Amount used :
20μg
Blocking :
5% skim milk in PBST, 25°C, 1Hr
Primary Antibody :
1:1000, 4°C, 16Hr
Lane Description :
HIF1 beta antibody detects HIF1 beta protein by western blot analysis. Un-treated (-) and treated (+, 1% O2 treatment ) B16 melanoma cell extracts (20 μg) were separated by SDS-PAGE.
