HIF1 beta antibody
|Applications||ICC/IF, IHC-P, WB|
Not tested in other applications.
|IHC (Formalin-fixed paraffin-embedded sections)||1:100-1:1000*
*Optimal dilutions/concentrations should be determined by the researcher.
|Positive Controls||293T , A431 , HeLa , HepG2 (untreated) , HepG2 ( 1% O2 treatment for 24hr)|
(Please refer to the vial label for the specific concentration)|
|Purification||Purified by antigen-affinity chromatography.|
|Full Name||aryl hydrocarbon receptor nuclear translocator|
|Product Description||Rabbit Polyclonal antibody to HIF1 beta (aryl hydrocarbon receptor nuclear translocator)|
|Background||The aryl hydrocarbon (Ah) receptor is involved in the induction of several enzymes that participate in xenobiotic metabolism. The ligand-free, cytosolic form of the Ah receptor is complexed to heat shock protein 90. Binding of ligand, which includes dioxin and polycyclic aromatic hydrocarbons, results in translocation of the ligand-binding subunit only to the nucleus. Induction of enzymes involved in xenobiotic metabolism occurs through binding of the ligand-bound Ah receptor to xenobiotic responsive elements in the promoters of genes for these enzymes. This gene encodes a protein that forms a complex with the ligand-bound Ah receptor, and is required for receptor function. The encoded protein has also been identified as the beta subunit of a heterodimeric transcription factor, hypoxia-inducible factor 1 (HIF1). A t(1;12)(q21;p13) translocation, which results in a TEL-ARNT fusion protein, is associated with acute myeloblastic leukemia. Three alternatively spliced variants encoding different isoforms have been described for this gene. [provided by RefSeq]|
|Synonyms||HIF-1-beta Antibody, ARNT Antibody, HIF1-beta Antibody, bHLHe2 Antibody, aryl hydrocarbon receptor nuclear translocator Antibody, HIF-1beta Antibody, HIF1BETA Antibody, HIF1B Antibody, TANGO Antibody|
|Cellular Localization||Nucleus |
|Immunogen||Recombinant protein encompassing a sequence within the C-terminus region of human HIF1 beta. The exact sequence is proprietary.|
|Species Reactivity||Human, Mouse|
|Predicted Cross Reactivity species Predicted cross-reactivity:|
Predicted cross-reactivity is based on sequence homology, with greater than 80% immunogen sequence identity considered positive.
Please note that we are only able to guarantee products to work in applications and species in which they have been tested.
|Rat, Pig, Rabbit, Sheep, Bovine, Guinea pig|
|Predict Reactivity Note||Rat (82%), Pig (91%), Rabbit (86%), Sheep (91%), Bovine (92%), Guinea pig (92%)|