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Catalog Number
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GTX30646
(
0
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Product Name
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HIF1AN antibody
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| Applications | EM, ICC/IF, IHC, IHC-P, IP, WB |
| Application Note | Western Blot 1:1000-1:5000
Electron Microscopy
Immunocytochemistry/Immunofluorescence
Immunohistochemistry 1:100
Immunohistochemistry-Paraffin
Immunoprecipitation 1:10-1:500
This Factor Inhibiting HIF-1 antibody is useful for Immunohistochemistry, Immunoprecipitation and Western Blot. In WB, a band at ~40.3 kDa is seen. Use in Immunocytochemistry/immunofluorescence, Immunohistochemistry-Paraffin and Electron Microscopy reported in scientific literature (PMID 17003483)
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| Positive Controls | Rat astrocytes and C6 and U251 gliomas |
| Form Supplied | Liquid |
| Concentration | 1
mg/ml
(Please refer to the vial label for the specific concentration) |
| Full Name | hypoxia inducible factor 1, alpha subunit inhibitor |
| Background | Factor Inhibiting HIF1 (FIH1 or HIF1AN) protein facilitates repression of HIF-1 transcriptional activity by binding to VHL, which acts as a transcriptional corepressor. VHL inhibits HIF-1 alpha transactivation function by recruiting histone deacetylases. Involvement of VHL in association with FIH provides a unifying mechanism for the modulation of HIF-1 alpha protein stabilization and transcriptional activation in response to changes in cellular oxygen concentration. |
| Synonyms | DKFZp762F1811, FIH1, FLJ20615, FLJ22027 |
| Specificity | This antibody is specific for HIF1AN protein. |
| Host | Rabbit |
| Clonality | Polyclonal |
| Immunogen | A C-terminal region synthetic peptide made to the human HIF1AN protein sequence (between residues 300 and the C-terminus). |
| Antigen Species | Human |
| Species Reactivity | Human, Mouse, Rat |
| Conjugation | Unconjugated |