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Catalog Number
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GTX28327
(
0
)
|
|
Product Name
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HIV protease antibody [1696]
|
| Applications | Dot, ELISA, Inhibition assay, WB |
| Application Note | For ELISA: Use at an assay dependent dilution. For Inhib: Use at an assay dependent dilution. Inhibits the enzyme activity of HIV-1 and HIV-2 proteases. For WB (reducing conditions): Use at a concentration of 0 . 5 μ g / m l. Sample preparation: Resuspend approx. 50 mil. cells in 1 ml cold Lysis buffer (1% laurylmaltoside in 20 mM Tris/Cl, 100 mM NaCl pH 8.2, 50 mM NaF including Protease inhibitor Cocktail). Incubate 60 min on ice. Centrifuge to remove cell debris. Mix lysate with reducing Laemmli SDS-PAGE sample buffer. Optimal dilutions/concentrations should be determined by the researcher. |
| Concentration | 1
mg/ml
(Please refer to the vial label for the specific concentration) |
| Purification | Protein A affinity purified |
| Purification Note | > 95% (by SDS-PAGE) |